Volume 17, Number 8—August 2011
Dispatch
Bagaza Virus in Partridges and Pheasants, Spain, 2010
Montserrat Agüero, Jovita Fernández-Pinero, Dolores Buitrago, Azucena Sánchez, Maia Elizalde, Elena San Miguel, Ruben Villalba, Francisco Llorente, and Miguel Ángel Jiménez-Clavero
Table
Analysis of red-legged partridges (Alectoris rufa) and common pheasants (Phasianus colchicus) for WNV and BAGV, Cádiz, Spain, 2010*
| Species | No. animals analyzed | Sample type | No. positive/no. tested |
Virus isolation in ECE, no. samples positive/no. tested† | |
|---|---|---|---|---|---|
| WNV | BAGV | ||||
| A. rufa | 11 | Brain | 0/11 | 11/11 | 1/3 (AF) |
| Cloacal swab | 0/2 | 1/2 | NA | ||
| Oral swab | 0/2 | 1/2 | NA | ||
| Gut | 0/1 | 1/1 | NA | ||
| Heart‡ | 0/1 | 1/1 | 1/3 (AF) | ||
| Kidney‡ | 0/1 | 1/1 | 2/3 (AF, CM, and VS) | ||
| Lung | 0/1 | 1/1 | NA | ||
| Liver | 0/1 | 1/1 | NA | ||
| Blood | 0/1 | 1/1 | NA | ||
| Feathers§ |
0/3 |
3/3 |
NA |
||
| P. colchicus | 2 | Brain | 0/2 | 2/2 | NA |
*West Nile virus (WNV) or Bagaza virus (BAGV) nucleic acids were identified by using real-time reverse transcription PCR (RT-PCR) or panflaviviral heminested RT-PCR, respectively, and amplicon (214 bp) sequencing. ECE, embryonated chicken egg; AF, allantoic fluid; NA, not analyzed; CM, chorioallantoic fluid; VS, viscera.
†ECEs were infected with homogenates of various samples.
‡Samples from which full-length sequence of BAGV was obtained.
§Tail, breast, and wing feathers from 1 partridge were analyzed.
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