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Volume 18, Number 12—December 2012
Research

Borrelia, Rickettsia, and Ehrlichia Species in Bat Ticks, France, 2010

Cristina Socolovschi, Tahar Kernif, Didier Raoult, and Philippe ParolaComments to Author 
Author affiliations: Author affiliations: Unité de Recherche en Maladies Infectieuses et Tropicales Emergentes, Marseille, France (C. Socolovschi, T. Kernif, D. Raoult); World Health Organization Collaborative Center for Rickettsial Diseases and Other Arthropod-Borne Bacterial Diseases, Marseille (P. Parola)

Main Article

Table

Primers and probes used to detect and confirm the presence of bacteria in Argas vespertilionis ticks collected in 2010 from an attic in France*

Bacterial species Gene target (ref) Primers and probe
Screening
Rickettsia RC0338 (12) 1029-F1: 5′-GAM AAA TGA ATT ATA TAC GCC GCA AA-3′
1029-R1: 5′-ATT ATT KCC AAA TAT TCG TCC TGT AC-3′
1029–1P: 6FAM-CTC AAG ATA AGT ATG AGT TAA ATG TAA A-TAMRA
Borrelia 16S rRNA (13) Bor_16S_3_F: 5′ AGC CTT TAA AGC TTC GCT TGT AG 3′
Bor_16S_3_R: 5′ GCC TCC CGT AGG AGT CTG G 3′
Bor_16S_3_P: 6FAM- CCG GCC TGA GAG GGT GAA CGG TAMRA
Coxiella burnetii IS1111 (14) IS 1111F: 5′-CAA GAA ACG TAT CGC TGT GGC-3′
IS 1111R: 5′-CAC AGA GCC ACC GTA TGA ATC-3′
IS1111P: 6-FAM-CCG AGT TCG AAA CAA TGA GGG CTG-TAMRA
Bartonella ITS (15) Barto ITS3 F: 5′-GAT GCC GGG GAA GGT TTT C-3′
Barto ITS3 R: 5′-GCC TGG GAG GAC TTG AAC CT-3′
Barto ITS3 P: 6 FAM-GCG CGC GCT TGA TAA GCG TG-TAMRA
Ehrlichia 16S rRNA (16) Erli_16S_F: 5′-GGT-ACC-YAC-AGA-AGA-AGT-CC-3′
Erli_16S_R: 5′-TAG-CAC-TCA-TCG-TTT-ACA-GC-3′
Confirmation
Rickettsia gltA (17) 409D: 5′-CCT ATG GCT ATT ATG CTT GC-3′
1258R: 5′-ATT GCA AAA AGT ACA GTG AAC A-3′
Rickettsia ompA (17) 190–70: 5′-ATG GCG AAT ATT TCT CCA AAA-3′
190–701: 5′- GTT CCG TTAATGGCAGCA TCT-3′
190–180: 5′- GCA GCG ATA ATG CTG AGT A-3′
Borrelia 16S rRNA (13) BF1: 5′-GCT GGC AGT GCG TCT TAA GC-3′
BR1: 5′-GCT TCG GGT ATC CTC AAC TC-3′
Borrelia Flagellin (18) Bor1: 5′- TAA TAC GTC AGC CAT AAA TGC-3′
Bor2: 5′- GCT CTT TGA TCA GTT ATC ATT C-3′
C. burnetii IS30 (14) IS30a F: 5′-CGC TGA CCT ACA GAA ATA TGT CC-3′
IS30a R: 5′-GGG GTA AGT AAA TAA TAC CTT CTG G-3′
IS30a P: 6-FAM-CAT GAA GCG ATT TAT CAA TAC GTG TAT GC-TAMRA
Bartonella ITS (15) Urbarto1: 5′-CTT-CGT-TTC-TCT-TTC-TTC-A-3′
Urbarto2: 5′-CTT-CTC-TTC-ACA-ATT-TCA-AT-3′
Ehrlichia gltA (19) HER-CS133F: 5′-GGW TTY ATG TCY ACT GCT GC-3′
HER-CS778R: 5′-GCN CCM CCA TGM GCT GG-3′

*Ref, reference. Primers in boldface were used for sequencing.

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