Volume 18, Number 12—December 2012
Dispatch
MRSA Variant in Companion Animals
Table
Characteristics of 10 methicillin-resistant Staphylococcus aureus isolates harboring mecALGA251 obtained from companion animals, Germany, 2008–2011*
IMT no. | Original no. | Year isolated | Host | Site | Clinical signs | Free-ranging animal | Federal state | OXA MIC† | spa type‡ | ST§ |
---|---|---|---|---|---|---|---|---|---|---|
17403 | VB 999987 | 2008 | Cat | Eye | Purulent infection | Yes | Rhineland-Palatinate | ≥4 | t10033 | 1945 |
21135 | VB 964992 | 2010 | Cat | Wound | Lymphadenitis | Yes | Bavaria | ≥4 | t843 | 130 |
21231 | VB 971931 | 2010 | Cat | Skin | Dermatitis | Unknown | Hessia | 1 | t1773 | 130 |
24068 | VB 961584 | 2010 | Cat | Tachea | Stridor | Yes | Hessia | 0.5 | t10006 | 599 |
25044 | VB 969929 | 2010 | Dog | Abscess | Tumor, dolor | No | Bavaria | 2 | t1694 | 599 |
25147 | VB 969572–2 | 2010 | Cat | Wound | Suture dehiscence | Yes | North Rhine-Westphalia | 2 | t278 | 599 |
25470 | VB 972406 | 2010 | Dog | Eye | Purulent infection | No | Bavaria | 2 | t1694 | 599 |
25715 | VB 969935 | 2010 | Guinea pig | Fistula | Purulent infection | No | Hessia | 2 | t843 | 130 |
28299 | VB 952042 | 2011 | Cat | Phlegmon | Dermatitis | Yes | Bavaria | 1 | t278 | 599 |
28429 | IMT 2272/11 | 2011 | Cat | Abscess | Fever | Yes | Berlin | ≥4 | t10009 | 130 |
*IMT, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany; OXA, oxacillin; ST, sequence type; VB, Vet Med Labor GmbH, Ludwigsburg, Germany. All isolates were positive for nuc and negative for mecA according to PCR to detect MRSA (8), and all were positive for mecALGA251 according to PCR to detect the novel mecA homologue (5).
†Detected by the VITEK 2 system (bioMérieux, Nürtingen, Germany).
‡Spa repeats and spa type determined according to Harmsen et al. (11) and the Ridom SpaServer (http://spaserver.ridom.de).
§Determined according to Enright et al. (10).
References
- Wieler LH, Ewers C, Guenther S, Walther B, Lübke-Becker A. Methicillin-resistant staphylococci (MRS) and extended-spectrum beta-lactamases (ESBL)–producing Enterobacteriaceae in companion animals: nosocomial infections as one reason for the rising prevalence of these potential zoonotic pathogens in clinical samples. Int J Med Microbiol. 2011;301:635–41. DOIPubMedGoogle Scholar
- Pinho MG, de Lencastre H, Tomasz A. An acquired and a native penicillin-binding protein cooperate in building the cell wall of drug-resistant staphylococci. Proc Natl Acad Sci U S A. 2001;98:10886–91. DOIPubMedGoogle Scholar
- García-Alvarez L, Holden MT, Lindsay H, Webb CR, Brown DF, Curran MD, Meticillin-resistant Staphylococcus aureus with a novel mecA homologue in human and bovine populations in the UK and Denmark: a descriptive study. Lancet Infect Dis. 2011;11:595–603. DOIPubMedGoogle Scholar
- Shore AC, Deasy EC, Slickers P, Brennan G, O’Connell B, Monecke S, Detection of staphylococcal cassette chromosome mec type XI carrying highly divergent mecA, mecI, mecR1, blaZ, and ccr genes in human clinical isolates of clonal complex 130 methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother. 2011;55:3765–73. DOIPubMedGoogle Scholar
- Sung JM, Lloyd DH, Lindsay JA. Staphylococcus aureus host specificity: comparative genomics of human versus animal isolates by multi-strain microarray. Microbiology. 2008;154:1949–59. DOIPubMedGoogle Scholar
- Walther B, Monecke S, Ruscher C, Friedrich AW, Ehricht R, Slickers P, Comparative molecular analysis substantiates a zoonotic potential of equine methicillin- resistant Staphylococcus aureus (MRSA). J Clin Microbiol. 2009;47:704–10. DOIPubMedGoogle Scholar
- Clinical and Laboratory Standards Institute. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals. Approved standard. Wayne (PA); 2008.
- Monecke S, Kuhnert P, Hotzel H, Slickers P, Ehricht R. Microarray based study on virulence-associated genes and resistance determinants of Staphylococcus aureus isolates from cattle. Vet Microbiol. 2007;125:128–40. DOIPubMedGoogle Scholar
- Fitzgerald JR, Monday SR, Foster TJ, Bohach GA, Hartigan PJ, Meaney WJ, Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens. J Bacteriol. 2001;183:63–70. DOIPubMedGoogle Scholar
- Sasaki T, Tsubakishita S, Tanaka Y, Ohtsuka M, Hongo I, Fukata T, Population genetic structures of Staphylococcus aureus isolates from cats and dogs in Japan. J Clin Microbiol. 2012;50:2152–5.
- Rutland BE, Weese JS, Bolin C, Au J, Malani AN. Human-to-dog transmission of methicillin-resistant Staphylococcus aureus. Emerg Infect Dis. 2009;15:1328–30. DOIPubMedGoogle Scholar