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Volume 18, Number 8—August 2012


Lack of Evidence for Schmallenberg Virus Infection in Highly Exposed Persons, Germany, 2012

Tanja Ducomble1, Hendrik Wilking1Comments to Author , Klaus Stark, Anja Takla, Mona Askar, Lars Schaade, Andreas Nitsche, and Andreas Kurth
Author affiliations: Robert Koch Institute, Berlin, Germany (T. Ducomble, H. Wilking, K. Stark, A. Takla, M. Askar, L. Schaade, A. Nitsche, A. Kurth); European Program for Intervention Epidemiology Training, Stockholm, Sweden (T. Ducomble); and Postgraduate Training for Applied Epidemiology–German Field Epidemiology Training Program, Berlin (A. Takla, M. Askar)

Main Article

Table 2

Results of diagnostic tests for SBV in serum samples from exposed shepherds, Germany, 2012*

Test system and dilution Test results, no. (%)
Positive/total Indeterminate/total
IFAT IgG 80 0/60 (0) 0/60 (0)
IFAT IgM 80 0/60 (0) 0/60 (0)
IFAT IgG 20 0/60 (0) 1/60 (2)
IFAT IgM 20 0/60 (0) 7/60 (11)
SNT titer 10† 0/8 (0) 0/8 (0)
RT-qPCR 0/60 (0) 0/60 (0)

*SBV, Schmallenberg virus; IFAT, indirect fluorescent antibody test; SNT, serum neutralization test; RT-qPCR, quantitative reverse transcription PCR.
†Performed in only 8 serum samples with IFAT indeterminate results; 2/8 (25%) reported symptoms as outlined in Table 1.

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1These authors contributed equally to this article.