Volume 18, Number 9—September 2012
Letter
Entamoeba bangladeshi nov. sp., Bangladesh
Tricia L. Royer1, Carol Gilchrist1, Mamun Kabir1, Tuhinur Arju, Katherine S. Ralston, Rashidul Haque, C. Graham Clark, and William A. Petri
Table
Oligonucleotide primers used for screening and sequencing of Entamoeba bangladeshi nov. sp., Bangladesh*
| Target organism | Primer name | Primer sequence, 5′ → 3′ | Reference |
|---|---|---|---|
| Broad specificity Entamoeba sp. | Entagen-F | ACT TCA GGG GGA GTA TGG TCA C | (7) |
| Entagen-R | CAA GAT GTC TAA GGG CAT CAC AG | (7) | |
| E. histolytica | Eh-F | AAC AGT AAT AGT TTC TTT GGT TAG TAA AA | (9) |
| Eh-R | CTT AGA ATG TCA TTT CTC AAT TCA T | (9) | |
| Eh-YYT Probe | YYT-ATT AGT ACA AAA TGG CCA ATT CAT TCA-Dark Quencher | (9) | |
| E. moshkovskii | Em-1 | CTC TTC ACG GGG AGT GCG | (8) |
| Em-2 | TCG TTA GTT TCA TTA CCT | (8) | |
| nEm-1 | GAA TAA GGA TGG TAT GAC | (8) | |
| nEm-2 | AAG TGG AGT TAA CCA CCT | (8) | |
| E. dispar | E-1 | TTT GTA TTA GTA CAA A | (10) |
| E-2 | GTA [A/G]TA TTG ATA TAC T | (10) | |
| Ed-1 | AGT GGC CAA TTT ATG TAA GT | (10) | |
| Ed-2 | TTT AGA AAC AAT GTT TCT TC | (10) |
*Boldface indicates the probe fluorophore and quencher.
References
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1These authors contributed equally to this article.
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