Figure. . Partial RNA-dependent RNA polymerase (RdRp) gene phylogeny, including the novel betacoronavirus from a Neoromicia zuluensis bat in South Africa (GenBank accession no. KC869678 for both partial RdRp and spike gene sequences). The Bayesian phylogeny was done on a translated 816-nt RdRp gene sequence fragment, as described (5). MrBayes V3.1 (http://mrbayes.sourceforge.net/) was used with a WAG substitution model assumption over 2,000,000 generations sampled every 100 steps, resulting in 20,000 trees, of which 25% were discarded as burn-in. A whale gammacoronavirus was used as an outgroup. The novel N. zuluensis bat virus is highlighted in gray. Values at deep nodes represent statistical support from posterior probabilities. Only values >0.9 are shown. Coronavirus clades are depicted to the right of taxa. Scale bar represents genetic distance. MERS-CoV, Middle East respiratory syndrome coronavirus; SARS, severe acute respiratory syndrome; Bt-CoV, bat coronavirus; HCoV, human coronavirus, MHV, mouse hepatitis virus; FCoV, feline coronavirus; TGEV, transmissible gastroenteritis coronavirus.