Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 19, Number 12—December 2013
Research

Spontaneous Generation of Infectious Prion Disease in Transgenic Mice

Juan-María TorresComments to Author , Joaquín Castilla, Belén Pintado, Alfonso Gutiérrez-Adan, Olivier Andréoletti, Patricia Aguilar-Calvo, Ana-Isabel Arroba, Beatriz Parra-Arrondo, Isidro Ferrer, Jorge Manzanares, and Juan-Carlos Espinosa
Author affiliations: Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Madrid, Spain (J.-M. Torres, J. Castilla, B. Pintado, A. Gutiérrez-Adán P. Aguilar-Calvo, A.-I. Arroba, B. Parra-Arrondo, J.-C. Espinosa); Basque Foundation for Science, Bilbao, Spain (J. Castilla); Ecole Nationale Vétérinaire de Toulouse, Toulouse, France (O. Andréoletti); Hospitalet de Llobregat, Barcelona, Spain (I. Ferrer); Universidad Miguel Hernandez, Sant Joan d´Alacant, Spain (J. Manzanares)

Main Article

Figure 4

Comparative Western blot analyses of brain prion protein resistant to proteinase K digestion (PrPres) from BoPrP-Tg110 mice infected with bovine spongiform encephalopathy (BSE)-C, 113L-BSE, BSE-L, and BSE-H prions. Mice infected with newly generated 113L-BSE prion at first (P1), second (P2), and third (P3) passages are compared with mice infected with BSE-C (P1) (lane 4); BSE-L (P1) (lane 5); and BSE-H (P1) (lane 6) prions. Each panel was identified by using the monoclonal antibody (mAb) listed

Figure 4. . . Comparative Western blot analyses of brain prion protein resistant to proteinase K digestion (PrPres) from BoPrP-Tg110 mice infected with bovine spongiform encephalopathy (BSE)-C, 113L-BSE, BSE-L, and BSE-H prions. Mice infected with newly generated 113L-BSE prion at first (P1), second (P2), and third (P3) passages are compared with mice infected with BSE-C (P1) (lane 4); BSE-L (P1) (lane 5); and BSE-H (P1) (lane 6) prions. Each panel was identified by using the monoclonal antibody (mAb) listed at the bottom left. The same quantities of PrPres were loaded in all lanes. BoPrP, bovine prion protein; 113L, leucine substitution at codon 113. Values on the right are molecular masses in kilodaltons.

Main Article

Page created: November 19, 2013
Page updated: November 19, 2013
Page reviewed: November 19, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external