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Volume 19, Number 8—August 2013
Dispatch

Primary and Secondary Human Bocavirus 1 Infections in a Family, Finland

Alma Jula, Matti Waris, Kalle Kantola, Ville Peltola, Maria Söderlund-Venermo, Klaus Hedman, and Olli RuuskanenComments to Author 
Author affiliations: Turku University Hospital, Turku, Finland (A. Jula, V. Peltola, O. Ruuskanen); University of Turku, Turku (M. Waris); University of Helsinki, Helsinki, Finland (K. Kantola, M. Söderlund-Venermo, K. Hedman)

Main Article

Table

HBoV1 DNA, other respiratory viruses, and HBoV antibodies in family members during 7 months, Finland*

Patient and no. days after hospital admission of index patient Symptoms of acute infection HBoV1 DNA in NPA, copies/mL HBoV1 DNA in serum, copies/mL† HBoV1 IgM‡ HBoV IgG avidity HBoV1 IgG‡ HBoV2 IgG‡ HBoV3 IgG‡§ Other viruses detected in NPA by PCR
Index
1 Yes 1.6 × 1010 NT NT NT NT NT NT None
4 Yes 4.1 × 108 3,000 2.148 2.0 1.034 0 0 HRV
9 Yes 3.0 × 105 1,400 2.089 2.0 3.127 0 0.034 HRV negative
29 Yes 300 440 1.613 9.7 3.028 0.060 0 HRV negative
64 No NT Negative 0.154 17.1 3.426 0.050 0 NT
74 No 5,400 NT NT NT NT NT NT None
109 Yes 600 Negative 0.040 31.3 3.221 0 0 PIV1
185 ND Negative NT NT NT NT NT NT NT
232
 Yes
 Negative
 NT
 NT
 NT
 NT
 NT
 NT
 AdV
Twin brother
5 Yes 6,300 NT NT NT NT NT NT HRV
14 ND 7,800 NT NT NT NT NT NT HRV
29 ND 3,900 NT NT NT NT NT NT HRV
64 No NT Negative 0.022 26.9 3.305 0 0 None
74 No Negative NT NT NT NT NT NT None
109 Yes 99,000 Negative 0.011 38.2 3.242 0 0 PIV1
232
 Yes
 5,700
 NT
 NT
 NT
 NT
 NT
 NT
 AdV
Mother
5 No Negative NT NT NT NT NT NT HRV
13 ND Negative NT 0.013 50.2 0 0.509 0.307 HRV negative
30 No Negative NT 0.019 45.0 0.001 0.542 0.360 HRV negative
64 No Negative NT 0.019 48.1 0.012 0.480 0.259 NT
109
 No
 300
 NT
 0.032
 50.2
 0
 0.861
 0.585
 PIV1
Grandmother
5 Yes 5,100 NT NT NT NT NT NT None
13 ND 22,000 960 0.011 58.0 0.395 0.542 0.016 HRV negative
64 ND NT Negative 0.012 25.4 2.731 1.470 0 None
74 No 3,600 NT NT NT NT NT NT HRV negative
109 Yes 300 Negative 0.023 33.5 2.674 1.834 0 PIV1, HRV

*HBoV1, human bocavirus 1; NPA, nasopharyngeal aspirate; NT, not tested; HRV, human rhinovirus; HRV negative, tested by monoplex PCR only; PIV1, parainfluenzavirus type 1; ND, no data available; AdV, adenovirus; none, negative by multiplex PCR.
†All serum samples were negative for HBoV2–4 DNA (6).
‡HBoV antibodies after heterologous antigen competition are shown as absorbance values at 492 nm, cutoff 0.13 (7).
§IgG avidity was calculated as the ratio of 2 IgG end-point titers (urea positive/urea negative) as described (8). Results obtained with unblocked enzyme immunoassay and confirmed with specific competition enzyme immunoassays.

Main Article

References
  1. Jartti  T, Hedman  K, Jartti  L, Ruuskanen  O, Allander  T, Söderlund-Venermo  M. Human bocavirus–the first 5 years. Rev Med Virol. 2012;22:46–64. DOIPubMedGoogle Scholar
  2. Meriluoto  M, Hedman  L, Tanner  L, Simell  V, Mäkinen  M, Simell  S, Association of human bocavirus 1 infection with respiratory disease in childhood follow-up study, Finland. Emerg Infect Dis. 2012;18:264–71. DOIPubMedGoogle Scholar
  3. Longtin  J, Bastien  M, Gilca  R, Leblanc  E, de Serres  G, Bergeron  MG, Human bocavirus infections in hospitalized children and adults. Emerg Infect Dis. 2008;14:217–21. DOIPubMedGoogle Scholar
  4. Martin  ET, Fairchok  MP, Kuypers  J, Magaret  A, Zerr  DM, Wald  A, Frequent and prolonged shedding of bocavirus in young children attending daycare. J Infect Dis. 2010;201:1625–32. DOIPubMedGoogle Scholar
  5. Christensen  A, Nordbø  SA, Krokstad  S, Rognlien  AG, Døllner  H. Human bocavirus in children: mono-detection, high viral load and viraemia are associated with respiratory tract infection. J Clin Virol. 2010;49:158–62. DOIPubMedGoogle Scholar
  6. Kantola  K, Sadeghi  M, Antikainen  J, Kirveskari  J, Delwart  E, Hedman  K, Real-time quantitative PCR detection of four human bocaviruses. J Clin Microbiol. 2010;48:4044–50. DOIPubMedGoogle Scholar
  7. Kantola  K, Hedman  L, Arthur  J, Alibeto  A, Delwart  E, Jartti  T, Seroepidemiology of human bocaviruses 1–4. J Infect Dis. 2011;204:1403–12. DOIPubMedGoogle Scholar
  8. Hedman  L, Söderlund-Venermo  M, Jartti  T, Ruuskanen  O, Hedman  K. Dating of human bocavirus infection with protein-denaturing IgG-avidity assays– secondary immune activations are ubiquitous in immunocompetent adults. J Clin Virol. 2010;48:44–8. DOIPubMedGoogle Scholar
  9. Koskenvuo  M, Möttönen  M, Waris  M, Allander  T, Salmi  TT, Ruuskanen  O. Human bocavirus in children with acute lymphoblastic leukemia. Eur J Pediatr. 2008;167:1011–5. DOIPubMedGoogle Scholar
  10. Peltola  V, Waris  M, Österback  R, Susi  P, Ruuskanen  O, Hyypiä  T. Rhinovirus transmission within families with children: incidence of symptomatic and asymptomatic infections. J Infect Dis. 2008;197:382–9. DOIPubMedGoogle Scholar
  11. Söderlund-Venermo  M, Lahtinen  A, Jartti  T, Hedman  L, Kemppainen  K, Lehtinen  P, Clinical assessment and improved diagnosis of bocavirus-induced wheezing in children, Finland. Emerg Infect Dis. 2009;15:1423–30. DOIPubMedGoogle Scholar
  12. Ursic  T, Steyer  A, Kopriva  S, Kalan  G, Krivec  U, Petrovec  M. Human bocavirus as the cause of a life-threatening infection. J Clin Microbiol. 2011;49:1179–81. DOIPubMedGoogle Scholar
  13. Edner  N, Castillo-Rodas  P, Falk  L, Hedman  K, Söderlund-Venermo  M, Allander  T. Life-threatening respiratory tract disease with human bocavirus-1 infection in a 4-year-old child. J Clin Microbiol. 2012;50:531–2. DOIPubMedGoogle Scholar
  14. Körner  RW, Söderlund-Venermo  M, van Koningsbruggen-Rietschel  S, Kaiser  R, Malecki  M, Schildgen  O. Severe human bocavirus infection, Germany. Emerg Infect Dis. 2011;17:2303–5. DOIPubMedGoogle Scholar
  15. Morens  DM, Burke  DS, Halstead  SB. The wages of original antigenic sin. Emerg Infect Dis. 2010;16:1023–4. DOIPubMedGoogle Scholar

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Page updated: July 19, 2013
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