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Volume 19, Number 9—September 2013
Research

Use of Staged Molecular Analysis to Determine Causes of Unexplained Central Nervous System Infections

Chien-Chin HsuComments to Author , Rafal Tokarz, Thomas Briese, Hung-Chin Tsai, Phenix-Lan Quan, and W. Ian Lipkin
Author affiliations: Columbia University, New York, New York, USA (C.-C. Hsu, R. Tokarz, T. Briese, P.-L. Quan, W.I. Lipkin); Southern Taiwan University of Science and Technology, Tainan, Taiwan (C.-C. Hsu); Chi-Mei Medical Center, Tainan (C.-C. Hsu); National Yang-Ming University Kaohsiung, Taiwan (H.-C. Tsai); Kaohsiung Veterans General Hospital, Kaohsiung (H.-C. Tsai)

Main Article

Table 3

Pathogens identified by a staged molecular approach

Pathogen No. (%) cases
 No. HIV positive Molecular method
Total Meningitis Encephalitis
Virus
Enterovirus* 2 (6) 2 (8) 0 0 MassTag PCR
Human herpesvirus 1 2 (6) 0 2 (33) 0 MassTag PCR/microarray†
Human herpesvirus 2 1 (3) 1 (4) 0 0 MassTag PCR
Varicella-zoster virus 3 (10) 0 3 (50) 0 MassTag PCR
Epstein-Barr virus 16 (52) 16 (64) 0 9 MassTag PCR
Cytomegalovirus
 1 (3)
 0
 1 (17)
 0
 MassTag PCR
Bacteria
Mycobacterium tuberculosis 1 (3) 1 (4) 0 0 MassTag PCR
Escherichia coli
 5 (16)
 5 (20)
 0
 1
 16S rRNA PCR
Total 31 (100) 25 (100) 6 (100) 10

*Sequences of enteroviruses showed 1 infection each of echovirus 11 and echovirus 30.
†For 1 case, human herpesvirus 1 was identified by MassTag PCR; in the other, by DNA microarray.

Main Article

Page created: August 20, 2013
Page updated: August 20, 2013
Page reviewed: August 20, 2013
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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