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Volume 2, Number 1—January 1996
Perspective

Molecular Population Genetic Analysis of Emerged Bacterial Pathogens: Selected Insights

James M. MusserComments to Author 
Author affiliation: Department of Pathology, Baylor College of Medicine, Houston, Texas, USA

Main Article

Table

Representative Emerged Bacterial Pathogens Investigated with Molecular Population Genetic Strategies

Organism Disease Reference
Borrelia species Lyme disease 146-149, 150,151
Escherichia coli O157:H7 hemorrhagic colitis; hemolytic uremic syndrome 33-35
Haemophilus influenzae Invasive disease 156
H. influenzae biogroup aegyptius Brazilian purpuric fever 25,26
Listeria monocytogenes Food-borne invasive disease 157, 158
Mycobacterium tuberculosis Tuberculosis 91,105
Neisseria meningitidis Sepsis, meningitis 52-63,66,69-73
Salmonella species Food-borne illness 159-161
Staphylococcus aureus Toxic shock syndrome; methicillin resistant strains 47,143,144
Streptococcus pyogenes Invasive disease, toxic-shock-like syndrome 82,83,85
S. pneumoniae Otitis, pneumonia, sepsis, meningitis 125-131,136
Vibrio cholerae Cholera 153-155

Main Article

References
  1. Milkman  R. Science. 1973;182:1024–6. Electrophoretic variation in Escherichia coli from natural sources. DOIPubMedGoogle Scholar
  2. Selander  RK, Levin  BR. Science. 1980;210:545–7.Genetic diversity and structure in Escherichia coli populations. DOIPubMedGoogle Scholar
  3. Lewontin  RC. The genetic basis of evolutionary change. New York: Columbia University Press, 1974.
  4. Kimura  M. The neutral theory of molecular evolution. New York: Cambridge University Press, 1983.
  5. Nei  M. Molecular evolutionary genetics. New York: Columbia University Press, 1987.
  6. Baumberg  S, Young  JPW, Wellington  EMH, Saunders  JR, eds. Population genetics of bacteria. Cambridge, UK: Cambridge University Press, 1995.
  7. Taylor  J, Powell  BW, Wright  J. Infantile diarrhoea and vomiting: a clinical and bacteriological investigation. [Clin Res]. BMJ. 1949;2:117–25. DOIPubMedGoogle Scholar
  8. Orskov  F, Orskov  I. J Infect Dis. 1983;148:346–57. Summary of a workshop on the clone concept in the epidemiology, taxonomy, and evolution of the Enterobacteriaceae and other bacteria.PubMedGoogle Scholar
  9. Orskov  F, Orskov  I, Evans  DJ, Sack  RB, Wadstrom  T. Special Escherichia coli serotypes among enterotoxigenic strains from diarrhoea in adults and children. Med Microbiol Immunol (Berl). 1976;162:73–80. DOIGoogle Scholar
  10. Lewontin  RC. Genetics. 1991;128:657–62. Twenty-five years ago in genetics: electrophoresis in the development of evolutionary genetics: milestone or millstone?PubMedGoogle Scholar
  11. Selander  RK, Caugant  DA, Ochman  H, Musser  JM, Gilmour  MN, Whittam  S. Appl Environ Microbiol. 1986;51:873–84. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics.PubMedGoogle Scholar
  12. Dykhuizen  D, Hartl  DL. Genetics. 1980;96:801–17. Selective neutrality of 6PGD allozymes in E. coli and the effects of genetic background.PubMedGoogle Scholar
  13. Whittam  TS, Ochman  H, Selander  RK. Proc Natl Acad Sci U S A. 1983;80:1751–5. Multilocus genetic structure in natural populations of Escherichia coli. DOIPubMedGoogle Scholar
  14. Dykhuizen  DE, de Framond  J, Hartl  DL. Selective neutrality of glucose-6-phosphate dehydrogenase allozymes in Escherichia coli. Mol Biol Evol. 1984;1:162–70.PubMedGoogle Scholar
  15. Ochman  H, Whittam  TS, Caugant  DA, Selander  RK. J Gen Microbiol. 1983;129:2715–26. Enzyme polymorphism and genetic structure in Escherichia coli and Shigella.PubMedGoogle Scholar
  16. Selander  RK, McKinney  RM, Whittam  TS, Bibb  WF, Brenner  DJ, Nolte  FS, J Bacteriol. 1985;163:1021–37. Genetic structure of populations of Legionella pneumophila.PubMedGoogle Scholar
  17. Musser  JM, Hewlett  EL, Peppler  MS, Selander  RKJ. Bacteriol. 1986;166:230–7. Genetic diversity and relationships in populations of Bordetella spp.
  18. Gilmour  MN, Whittam  TS, Kilian  M, Selander  RK. Genetic relationships among the oral streptococci. J Bacteriol 1987:5247- 57.
  19. Brenner  DJ, Steigerwalt  AG, Epple  P, Bibb  WF, McKinney  RM, Starnes  RW, Legionella pneumophila serogroup Lansing 3 isolated from a patient with fatal pneumonia, and descriptions of L. pneumophila subsp. pneumophila subsp. nov., L. pneumophila subsp. fraseri subsp. nov., and L. pneumophila subsp. pascullei subsp. nov. J Clin Microbiol. 1988;26:1695–703.PubMedGoogle Scholar
  20. Musser  JM, Barenkamp  SJ, Granoff  DM, Selander  RK. Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infect Immun. 1986;55:183–91.
  21. Quentin  R, Goudeau  A, Wallace  RJ Jr, Smith  AL, Selander  RK, Musser  JM. J Gen Microbiol. 1990;136:1203–9. Urogenital, maternal and neonatal isolates of Haemophilus influenzae: identification of unusually virulent serologically non-typable clone families and evidence for a new Haemophilus species.PubMedGoogle Scholar
  22. Versalovic  J, Swanson  DS, Musser  JM. Nucleic acid sequencing studies of microbial pathogens: insights into epidemiology, virulence, drug resistance, and diversity. In: Persing DH, editor. PCR Application for Emerging Infectious Diseases. Washington DC: American Society for Microbiology. In press.
  23. Brazilian Purpuric Fever Study Group. Brazilian purpuric fever: epidemic purpura fulminans associated with antecedent purulent conjunctivitis. Lancet. 1987;2:757–61.PubMedGoogle Scholar
  24. Brazilian Purpuric Fever Study Group. Haemophilus aegyptius bacteremia in Brazilian purpuric fever. Lancet. 1987;2:761–3.PubMedGoogle Scholar
  25. Brenner  DJ, Mayer  LW, Carlone  GM, J Clin Microbiol. 1988;26:1524–34. Biochemical, genetic, and epidemiologic characterization of Haemophilus influenzae biogroup aegyptius (H. aegyptius) strains associated with Brazilian purpuric fever.PubMedGoogle Scholar
  26. Musser  JM, Selander  RK. J Infect Dis. 1990;161:130–3. Brazilian purpuric fever: evolutionary genetic relationships of the case clone of Haemophilus influenzae biogroup aegyptius to encapsulated strains of Haemophilus influenzae.PubMedGoogle Scholar
  27. Mulks  MH, Kornfeld  SJ, Fragione  B, Plaut  AG. Relationship between the specificity of IgA proteases and serotype in Haemophilus influenzae. J Infect Dis. 1982;146:266–74.PubMedGoogle Scholar
  28. Carlone  GM, Gorelkin  L, Gheesling  LL, J Clin Microbiol. 1989;27:609–14. Potential virulence- associated factors in Brazilian purpuric fever.PubMedGoogle Scholar
  29. Rubin  LG. Phase-variable expression of the 145-kDa surface protein of Brazilian purpuric fever case-clone strains of Haemophilus influenzae biogroup aegyptius. J Infect Dis. 1995;171:713–7.PubMedGoogle Scholar
  30. Whitney  AM, Farley  MM. Infect Immun. 1993;:1559–62. Cloning and sequence analysis of the structural pilin gene of Brazilian purpuric fever-associated Haemophilus influenzae biogroup aegyptius.PubMedGoogle Scholar
  31. Riley  LW, Remis  RS, Helgerson  SD, Hemorrhagic colitis associated with a rare Escherichia coli serotype. N Engl J Med. 1983;308:681–5.PubMedGoogle Scholar
  32. Feng  P. Escherichia coli serotype O157:H7: novel vehicles of infection and emergence of phenotypic variants. Emerg Infect Dis. 1995;1:47–52. DOIPubMedGoogle Scholar
  33. Whittam  TS, Wachsmuth  IK, Wilson  RA. J Infect Dis. 1988;157:1124–33. Genetic evidence of clonal descent of Escherichia coli O157:H7 associated with hemorrhagic colitis and hemolytic uremic syndrome.PubMedGoogle Scholar
  34. Whittam  TS, Wolfe  ML, Wachsmuth  IK, Orskov  F, Orskov  I, Wilson  RA. Infect Immun. 1993;61:1619–29. Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea.PubMedGoogle Scholar
  35. Whittam  TS. Genetic population structure and pathogenicity in enteric bacteria. In: Baumberg S, Young JPW, Saunders SR, Wellington EMH, editors. Population genetics of bacteria. London: Cambridge University Press, 1995:217-45.
  36. Bohm  H, Karch  H. J Clin Microbiol. 1992;30:2169–72. DNA fingerprinting of Escherichia coli O157:H7 strains by pulsed-field gel electrophoresis.PubMedGoogle Scholar
  37. Yu  J, Kaper  JB. Mol Microbiol. 1992;6:411–7. Cloning and characterization of the eae gene of enterohemorrhagic Escherichia coli O157:H7. DOIPubMedGoogle Scholar
  38. Knutton  S, Baldwin  T, Williams  PH, McNeish  AS. Infect Immun. 1989;57:1290–8. Actin accumulation at sites of bacterial adhesion to tissue culture cells: basis of a new diagnostic test for enteropathogenic and enterohemorrhagic Escherichia coli.PubMedGoogle Scholar
  39. Law  D. Adhesion and its role in the virulence of enteropathogenic Escherichia coli. Clin Microbiol Rev. 1994;7:152–73.PubMedGoogle Scholar
  40. Selander  RK, Caugant  DA, Whittam  TS. Genetic structure and variation in natural populations of Escherichia coli. In: Neidhardt FC, Ingraham JL, Low KB, Magasanik B, Schaechter M, Umbarger HE, editors. Escherichia coli and Salmonella typhimurium: cellular and molecular biology. Washington, DC: American Society for Microbiology, 1987:1625-48.
  41. Caugant  DA, Levin  BR, Orskov  I, Orskov  F, Svanborg Eden  C, Selander  RK. Infect Immun. 1985;49:407–13. Genetic diversity in relation to serotype in Escherichia coli.PubMedGoogle Scholar
  42. Achtman  M, Pluschke  G. Clonal analysis of descent and virulence among selected Escherichia coli. Annu Rev Microbiol. 1986;40:185–210. DOIPubMedGoogle Scholar
  43. Todd  J, Fishaut  M, Kapral  F, Welch  T. Toxic-shock syndrome associated with phage-group-I staphylococci. Lancet. 1978;ii:1116–8. DOIGoogle Scholar
  44. Davis  JP, Chesney  PJ, Wand  PJ, LaVenture  M, and Investigation and Laboratory Team. Toxic-shock syndrome: epidemiologic features, recurrence, risk factors, and prevention. N Engl J Med. 1980;303:1429–35.PubMedGoogle Scholar
  45. Bonventre  PF, Weckbach  L, Harth  G, Haidaris  C. Rev Infect Dis. 1989;11:S90–6. Distribution and expression of toxic shock syndrome toxin 1 gene among Staphylococcus aureus isolates of toxic shock syndrome and non–toxic shock syndrome origin.PubMedGoogle Scholar
  46. deAzavedo  JCS. Animal models for toxic shock syndrome: overview. Rev Infect Dis. 1989;11:S205–9.PubMedGoogle Scholar
  47. Musser  JM, Schlievert  PM, Chow  AW, Proc Natl Acad Sci U S A. 1990;87:225–9. A single clone of Staphylococcus aureus causes the majority of cases of toxic shock syndrome. DOIPubMedGoogle Scholar
  48. See  RH, Chow  AW. Rev Infect Dis. 1989;11:S55–60. Microbiology of toxic shock syndrome: overview.PubMedGoogle Scholar
  49. Kreiswirth  BN. Rev Infect Dis. 1989;:S97–100. Genetics and expression of toxic shock syndrome toxin 1: overview.PubMedGoogle Scholar
  50. Lee  PK, Kreiswirth  BN, Deringer  JR, J Infect Dis. 1992;165:1056–63. Nucleotide sequences and biologic properties of toxic shock syndrome toxin 1 from bovine- and bovine-associated Staphylococcus aureus.PubMedGoogle Scholar
  51. Goh  S-H, Byrne  SK, Zhang  JL, Chow  AW. J Clin Microbiol. 1992;30:1642–5. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms.PubMedGoogle Scholar
  52. Caugant  DA, Froholm  LO, Bovre  K, Holten  E, Frasch  CE, Mocca  LF, Proc Natl Acad Sci U S A. 1986;83:4927–31. Intercontinental spread of a genetically distinctive complex of clones of Neisseria meningitidis causing epidemic disease. DOIPubMedGoogle Scholar
  53. Reeves  MW, Perkins  BA, Diermayer  M, Wenger  JD. Epidemic- associated Neisseria meningitidis detected by multilocus enzyme electrophoresis. Emerg Infect Dis. 1995;1:53–4. DOIPubMedGoogle Scholar
  54. Tavares Sacchi  C, Pessoa  LL, Ramos  SR, Ongoing group B Neisseria meningitidis epidemic in Sao Paulo, Brazil, due to increased prevalence of a single clone of the ET-5 complex. J Clin Microbiol. 1992;30:1734–8.PubMedGoogle Scholar
  55. Caugant  DA, Bol  P, Hoiby  EA, Zanen  HC, Froholm  LO. J Infect Dis. 1990;162:867–74. Clones of serogroup B Neisseria meningitidis causing systemic disease in the Netherlands, 1958-1986.PubMedGoogle Scholar
  56. Caugant  DA, Kristiansen  B-E, Froholm  LO, Bovre  K, Selander  RK. Infect Immun. 1988;56:2060–8. Clonal diversity of Neisseria meningitidis from a population of asymptomatic carriers.PubMedGoogle Scholar
  57. Ashton  FE, Ryan  JA, Borczyk  A, Caugant  DA, Mancino  L, Huang  D. J Clin Microbiol. 1991;29:2489–93. Emergence of a virulent clone of Neisseria meningitidis serotype 2a that is associated with meningococcal group C disease in Canada.PubMedGoogle Scholar
  58. Tavares Sacchi  C, Cobo Zanella  R, Caugant  DA, Emergence of a new clone of serogroup C Neisseria meningitidis in Sao Paulo, Brazil. J Clin Microbiol. 1992;30:1282–6.PubMedGoogle Scholar
  59. Jackson  LA, Schuchat  A, Reeves  MW, Wenger  JD. Serogroup C meningococcal outbreaks in the United States: an emerging threat. JAMA. 1995; 383–9. DOIPubMedGoogle Scholar
  60. Whalen  CM, Hockin  JC, Ryan  A, Ashton  F. The changing epidemiology of invasive meningococcal disease in Canada, 1985 through 1992: emergence of a virulent clone of Neisseria meningitidis. JAMA. 1995;273:390–4. DOIPubMedGoogle Scholar
  61. Olyhoek  T, Crowe  BA, Achtman  M. Clonal population structure of Neisseria meningitidis serogroup A isolated from epidemics and pandemics between 1915 and 1983. Rev Infect Dis. 1987;9:665–92.PubMedGoogle Scholar
  62. Achtman  M. Mol Microbiol. 1994;11:15–22. Clonal spread of serogroup A meningococci: a paradigm for the analysis of microevolution in bacteria. DOIPubMedGoogle Scholar
  63. Achtman  M. Trends Microbiol. 1995;3:186–92. Epidemic spread and antigenic variability of Neisseria meningitidis DOIPubMedGoogle Scholar
  64. Achtman  M, Kusecek  B, Morelli  G, J Infect Dis. 1992;165:53–68. A comparison of the variable antigens expressed by clone IV-1 and subgroup III of Neisseria meningitidis serogroup A.PubMedGoogle Scholar
  65. Crowe  BA, Wall  RA, Kusecek  B, J Infect Dis. 1989;159:686–700. Clonal and variable properties of Neisseria meningitidis isolated from cases and carriers during and after an epidemic in The Gambia, West Africa.PubMedGoogle Scholar
  66. Wang  J-F, Caugant  DA, Li  X, Hu  X, Poolman  JT, Crowe  BA, Clonal and antigenic analysis of serogroup A Neisseria meningitidis with particular reference to epidemiological features of epidemic meningitis in the People's Republic of China. Infect Immun. 1992;60:5267–82.PubMedGoogle Scholar
  67. Morelli  G, del Valle  J, Lammel  CJ, Mol Microbiol. 1994;11:175–87. Immunogenicity and evolutionary variability of epitopes within IgA1 proteases from serogroup A Neisseria meningitidis. DOIPubMedGoogle Scholar
  68. Suker  J, Feavers  IM, Achtman  M, Morelli  G, Wang  J-F, Maiden  MCJ. Mol Microbiol. 1994;12:253–65. The porAgene in serogroup A meningococci: evolutionary stability and mechanism of genetic variation DOIPubMedGoogle Scholar
  69. Caugant  DA, Mocca  LF, Frasch  CE, Froholm  LO, Zollinger  WD, Selander  RKJ. Bacteriol 1987;2781-92. Genetic structure of Neisseria meningitidis populations in relation to serogroup, serotype, and outer membrane protein pattern.
  70. Moore  PS, Reeves  MW, Schwartz  B, Gellin  BG, Broome  CV. Intercontinental spread of an epidemic group A Neisseria meningitidis strain. Lancet. 1989;2:260–3. DOIPubMedGoogle Scholar
  71. Riou  JY, Caugant  DA, Selander  RK, Poolman  JT, Guibourdenche  M, Collatz  E. Eur J Clin Microbiol Infect Dis. 1991;10:405–9. Characterization of Neisseria meningitidis serogroup A strains from an outbreak in France by serotype, serosubtype, multilocus enzyme genotype and outer membrane protein pattern. DOIPubMedGoogle Scholar
  72. Salih  MAM, Danielsson  D, Backman  A, Caugant  DA, Achtman  M, Olcen  P. J Clin Microbiol. 1990;28:1711–9. Characterization of epidemic and non-epidemic Neisseria meningitidis serogroup A strains from Sudan and Sweden.PubMedGoogle Scholar
  73. Pinner  RW, Onyango  F, Perkins  BA, Epidemic meningococcal disease in Nairobi, Kenya, 1989. J Infect Dis. 1992;166:359–64.PubMedGoogle Scholar
  74. Stevens  DL, Tanner  MH, Winship  J, Reappearance of scarlet fever toxin A among streptococci in the Rocky Mountain West: severe group A streptococcal infections associated with a toxic shock-like syndrome. N Engl J Med. 1989;321:1–7.PubMedGoogle Scholar
  75. Schwartz  B, Facklam  RR, Breiman  RF. Changing epidemiology of group A streptococcal infection in the USA. Lancet. 1990;336:1167–71. DOIPubMedGoogle Scholar
  76. Kohler  W, Gerlach  D, Knoll  H. Streptococcal outbreaks and erythrogenic toxin A. Zbl Bakt Hyg. 1987;266:104–15.
  77. Martin  PR, Hoiby  EA. Streptococcal serogroup A epidemic in Norway 1987-1988. Scand J Infect Dis. 1990;22:421–9. DOIPubMedGoogle Scholar
  78. Holm  SE, Norrby  A, Bergholm  AM, Norgren  M. Aspects of pathogenesis of serious group A streptococcal infections in Sweden, 1988-1989. J Infect Dis. 1992;166:31–7.PubMedGoogle Scholar
  79. Martin  DR, Single  LA. J Infect Dis. 1993;167:1112–7. Molecular epidemiology of group A streptococcus M type 1 infections.PubMedGoogle Scholar
  80. Carapetis  J, Robins-Browne  R, Martin  D, Shelby-James  T, Hogg  G. Increasing severity of invasive group A streptococcal disease in Australia: clinical and molecular epidemiological features and identification of a new virulent M-nontypeable clone. Clin Infect Dis. 1995;21:1220–7.PubMedGoogle Scholar
  81. The Working Group on Severe Streptococcal Infections. Defining the group A streptococcal toxic shock syndrome: rationale and consensus definition. JAMA. 1993;269:390–1. DOIPubMedGoogle Scholar
  82. Musser  JM, Hauser  AR, Kim  MH, Schlievert  PM, Nelson  K, Selander  RK. Proc Natl Acad Sci U S A. 1991;88:2668–72. Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression. DOIPubMedGoogle Scholar
  83. Musser  JM, Kapur  V, Szeto  J, Pan  X, Swanson  DS, Martin  DR. Infect Immun. 1995;:994–1003. Genetic diversity and relationships among Streptococcus pyogenes strains expressing serotype M1 protein: recent intercontinental spread of a subclone causing episodes of invasive disease.PubMedGoogle Scholar
  84. Nelson  K, Schlievert  PM, Selander  RK, Musser  JM. J Exp Med. 1991;174:1271–4. Characterization and clonal distribution of four alleles of the speA gene encoding pyrogenic exotoxin A (scarlet fever toxin) in Streptococcus pyogenes. DOIPubMedGoogle Scholar
  85. Musser  JM, Kapur  V, Kanjilal  S, J Infect Dis. 1993;167:337–46. Geographic and temporal distribution and molecular characterization of two highly pathogenic clones of Streptococcus pyogenes expressing allelic variants of pyrogenic exotoxin A (scarlet fever toxin).PubMedGoogle Scholar
  86. Marrack  P, Kappler  J. The staphylococcal enterotoxins and their relatives. Science. 1990;248:705–10. DOIPubMedGoogle Scholar
  87. Kline  JB, Collins  CM. Structure-function analysis of streptococcal pyrogenic exotoxin A (SpeA). In: Proceedings of the Annual Meeting of the American Society for Microbiology. Abstract B-59.
  88. Bloom  BR, Murray  CJL. Science. 1992;257:1055–64. Tuberculosis: commentary on a reemergent killer. DOIPubMedGoogle Scholar
  89. Centers for Disease Control. A strategic plan for the elimination of tuberculosis in the United States. MMWR 1989;38(suppl S-3):1- 25.
  90. Centers for Disease Control. Expanded tuberculosis surveillance and tuberculosis morbidity—United States, 1993. MMWR. 1994;43:361–6.PubMedGoogle Scholar
  91. Kapur  V, Whittam  TS, Musser  JM. Is Mycobacterium tuberculosis 15,000 years old? J Infect Dis. 1994;170:1348–9.PubMedGoogle Scholar
  92. Heym  B, Alzari  PM, Honore  N, Cole  ST. Mol Microbiol. 1995;15:235–45. Missense mutations in the catalase-peroxidase gene, katG, are associated with isoniazid- resistance in Mycobacterium tuberculosis. DOIPubMedGoogle Scholar
  93. Musser  JM. Antimicrobial agent resistance in mycobacteria: molecular genetic insights. Clin Microbiol Rev. 1995;8:496–514.PubMedGoogle Scholar
  94. Musser  JM, Kapur  V, Williams  DL, Kreiswirth  BN, van Soolingen  D, van Embden  JDA. Characterization of the catalase-peroxidase gene (katG) and inhA locus in isoniazid-resistant and -susceptible strains of Mycobacterium tuberculosis by automated DNA sequencing: restricted array of mutations associated with drug resistance. J Infect Dis. 1996;173:196–202.PubMedGoogle Scholar
  95. Wilson  TM, de Lisle  GW, Collins  DM. Mol Microbiol. 1995;15:1009–15. Effect of inhA and katG on isoniazid resistance and virulence of Mycobacterium bovis. DOIPubMedGoogle Scholar
  96. Ayvazian  LF. History of tuberculosis. In: Reichman LB, Hershfield ES, editors. Tuberculosis: a comprehensive international approach. New York: Marcel Dekker, 1993:1-20.
  97. Salo  WL, Aufderheide  AC, Buikstra  J, Holcomb  TA. Proc Natl Acad Sci U S A. 1994;91:2091–4. Identification of Mycobacterium tuberculosis DNA in a pre-Columbian Peruvian mummy. DOIPubMedGoogle Scholar
  98. Ross  BC, Raios  K, Jackson  K, J Clin Microbiol. 1992;30:942–6. Molecular cloning of a highly repeated DNA element from Mycobacterium tuberculosis and its use as an epidemiological tool.PubMedGoogle Scholar
  99. van Embden  JDA, Cave  MD, Crawford  JT, Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993;31:406–9.PubMedGoogle Scholar
  100. Webb  GB. Tuberculosis. New York: Hoeber, 1936.
  101. Telenti  A, Imboden  P, Marchesi  F, Lancet. 1993;341:647–50. Detection of rifampin- resistance mutations in Mycobacterium tuberculosis. DOIPubMedGoogle Scholar
  102. Kapur  V, Li  LL, Iordanescu  S, J Clin Microbiol. 1994;32:1095–8. Characterization by automated DNA sequencing of mutations in the gene (rpoB) encoding the RNA polymerase b subunit in rifampin-resistant Mycobacterium tuberculosis strains from New York City and Texas.PubMedGoogle Scholar
  103. Bloch  AB, Cauthen  GM, Onorato  IM, Nationwide survey of drug-resistant tuberculosis in the United States. JAMA. 1994;271:665–71. DOIPubMedGoogle Scholar
  104. Frieden  TR, Sterling  T, Pablos-Mendez  A, N Engl J Med. 1993;328:521–6. The emergence of drug-resistant tuberculosis in New York City. DOIPubMedGoogle Scholar
  105. Bifani  P, Plikaytis  BB, Kapur  V, Stockbauer  K, Pan  X, Lutfey  ML, JAMA. In press.
  106. Sullivan  EA, Kreiswirth  BN, Palumbo  L, Kapur  V, Musser  JM, Ebrahimzadeh  A, Lancet. 1995;345:1148–50. Emergence of fluoroquinolone- resistant tuberculosis in New York City. DOIPubMedGoogle Scholar
  107. Plikaytis  BB, Marden  JL, Crawford  JT, J Clin Microbiol. 1994;32:1542–6. Multiplex PCR assay specific for the multidrug-resistant strain W of Mycobacterium tuberculosis.PubMedGoogle Scholar
  108. Valway  SE, Greifinger  RB, Papania  M, Multidrug-resistant tuberculosis in the New York State prison system, 1990-1991. J Infect Dis. 1994;170:151–6.PubMedGoogle Scholar
  109. Longuet  P, Pierre  J, Lacassin  F, A limited multidrug- resistant Mycobacterium tuberculosis (MDR-TB) outbreak. Screening of hospitalized patients (pts). Presented at the 35th Interscience Conference on Antimicrobial Agents and Chemotherapy, San Francisco, CA, 1995;Abstract J59.
  110. Chretien  J, Holland  W, Macklem  P, Murray  P, Woolcock  A. Acute respiratory infections in children: a global public-health problem. N Engl J Med. 1984;310:982–4.PubMedGoogle Scholar
  111. Monto  AS. Acute respiratory infection in children of developing countries: challenge of the 1990s. Rev Infect Dis. 1989;11:498–505.PubMedGoogle Scholar
  112. Shann  F. Etiology of severe pneumonia in children in developing countries. Pediatr Infect Dis. 1986;5:247–52.PubMedGoogle Scholar
  113. Williams  WW, Hickson  MA, Kane  MA, Kendal  AP, Spika  JS, Hinman  AR. Immunization policies and vaccine coverage among adults. Ann Intern Med. 1988;108:616–25.PubMedGoogle Scholar
  114. Appelbaum  PC. Clin Infect Dis. 1992;15:77–83. Antimicrobial resistance in Streptococcus pneumoniae: an overview.PubMedGoogle Scholar
  115. Fenoll  A, Martin-Bourgon  C, Munoz  R, Vicioso  D, Casal  J. Rev Infect Dis. 1991;13:56–60. Serotype distribution and antimicrobial resistance of Streptococcus pneumoniae isolates causing systemic disease in Spain, 1979-1989.PubMedGoogle Scholar
  116. Marton  A. Clin Infect Dis. 1992;15:106–11. Pneumococcal antimicrobial resistance: the problem in Hungary.PubMedGoogle Scholar
  117. Jabes  D, Nachman  S, Tomasz  A. J Infect Dis. 1989;159:16–25. Penicillin-binding protein families: evidence for the clonal nature of penicillin resistance in clinical isolates of pneumococci.PubMedGoogle Scholar
  118. Dowson  CG, Coffey  TJ, Kell  C, Whiley  RA. Mol Microbiol. 1993;9:635–43. Evolution of penicillin resistance in Streptococcus pneumoniae; the role of Streptococcus mitis in the formation of a low affinity PBP2B in S. pneumoniae. DOIPubMedGoogle Scholar
  119. Dowson  CG, Hutchison  A, Spratt  BG. Mol Microbiol. 1989;3:95–102. Extensive re-modelling of the transpeptidase domain of penicillin-binding protein 2B of a penicillin-resistant South African isolate of Streptococcus pneumoniae. DOIPubMedGoogle Scholar
  120. Dowson  CG, Hutchison  A, Brannigan  JA, George  RC, Hansman  D, Linares  J, Proc Natl Acad Sci U S A. 1989;86:8842–6. Horizontal transfer of penicillin-binding protein genes in penicillin- resistant clinical isolates of Streptococcus pneumoniae. DOIPubMedGoogle Scholar
  121. Dowson  CG, Hutchison  A, Woodford  N, Johnson  AP, George  RC, Spratt  BG. Proc Natl Acad Sci U S A. 1990;87:5858–62. Penicillin-resistant viridans streptococci have obtained altered penicillin-binding protein genes from penicillin-resistant strains of Streptococcus pneumoniae. DOIPubMedGoogle Scholar
  122. Laible  G, Hakenbeck  R, Sicard  MA, Joris  B, Ghuysen  J-M. Mol Microbiol. 1989;3:1337–48. Nucleotide sequences of the pbpX genes encoding the penicillin- binding proteins 2x from Streptococcus pneumoniae R6 and a cefotaxime-resistant mutant, C506. DOIPubMedGoogle Scholar
  123. Laible  G, Spratt  BG, Hakenbeck  R. Mol Microbiol. 1991;5:1993–2002. Interspecies recombinational events during the evolution of altered PBP 2x genes in penicillin- resistant clinical isolates of Streptococcus pneumoniae. DOIPubMedGoogle Scholar
  124. Humbert  O, Prudhomme  M, Hakenback  R, Dowson  CG, Claverys  J-P. Proc Natl Acad Sci U S A. 1995;92:9052–6. Homologous recombination and mismatch repair during transformation in Streptococcus pneumoniae: saturation of the hex mismatch repair system. DOIPubMedGoogle Scholar
  125. Munoz  R, Coffey  TJ, Daniels  M, Dowson  CG, Laible  G, Casal  J, J Infect Dis. 1991;164:302–6. Intercontinental spread of a multiresistant clone of serotype 23F Streptococcus pneumoniae.PubMedGoogle Scholar
  126. Munoz  R, Musser  JM, Crain  M, Briles  DE, Marton  A, Parkinson  AJ, Clin Infect Dis. 1992;5:112–8. Geographic distribution of penicillin- resistant clones of Streptococcus pneumoniae: characterization by penicillin-binding protein profile, surface protein A typing, and multilocus enzyme analysis.
  127. Versalovic  J, Kapur  V, Mason  EO Jr, Shah  U, Koeuth  T, Lupski  JR, J Infect Dis. 1993;167:850–6. Penicillin-resistant Streptococcus pneumoniae strains recovered in Houston: identification and molecular characterization of multiple clones.PubMedGoogle Scholar
  128. Soares  S, Kristinsson  KG, Musser  JM, Tomasz  A. J Infect Dis. 1993;168:158–63. Evidence for the introduction of a multiresistant clone of serotype 6B Streptococcus pneumoniae from Spain to Iceland in the late 1980s.PubMedGoogle Scholar
  129. Sibold  C, Wang  J, Henrichsen  J, Hakenbeck  R. Infect Immun. 1992;60:4119–26. Genetic relationships of penicillin-susceptible and -resistant Streptococcus pneumoniae strains isolated on different continents.PubMedGoogle Scholar
  130. Kell  CM, Jordens  JZ, Daniels  M, Coffey  TJ, Bates  J, Paul  J, Infect Immun. 1993;61:4382–91. Molecular epidemiology of penicillin-resistant pneumococci isolated in Nairobi, Kenya.PubMedGoogle Scholar
  131. McDougal  LK, Facklam  R, Reeves  M, Hunter  S, Swenson  JM, Hill  BC, Antimicrob Agents Chemother. 1992;36:2176–84. Analysis of multiply antimicrobial-resistant isolates of Streptococcus pneumoniae from the United States.PubMedGoogle Scholar
  132. Kristinsson  KG, Hjalmarsdottir  MA, Steingrimsson  O. Increasing penicillin resistance in pneumococci in Iceland. Lancet. 1992;339:1606–7. DOIPubMedGoogle Scholar
  133. Nordic Statistics on Medicines 1987-1989. Uppsala, Sweden: Nordiska Lakemedelsnamnden, Nordic Council on Medicines, 1990: 66-71. NLN publication no. 30.
  134. Col  NF, O'Connor  RW. Estimating worldwide current antibiotic usage: report of task force 1. Rev Infect Dis. 1987;9(suppl 3):S232–9.PubMedGoogle Scholar
  135. Coffey  TJ, Dowson  CG, Daniels  M, Zhou  J, Martin  C, Spratt  BG, Mol Microbiol. 1991;5:2255–60. Horizontal transfer of multiple penicillin-binding protein genes, and capsular biosynthetic genes, in natural populations of Streptococcus pneumoniae. DOIPubMedGoogle Scholar
  136. Lomholt  H. Infect Immun. 1995;63:4238–43. Evidence of recombination and an antigenically diverse immunoglobulin A1 protease among strains of Streptococcus pneumoniae.PubMedGoogle Scholar
  137. Jevons  MP. "Celbenin"-resistant staphylococci. BMJ. 1961;1:124–5. DOIGoogle Scholar
  138. Grubb  WB. Molecular epidemiology of methicillin-resistant Staphylococcus aureus. In: Novick R, Skurray RA, editors. Molecular biology of the staphylococci. New York: VCH Publishers, 1990:595-606.
  139. Hartman  BJ, Tomasz  AJ. Bacteriol. 1984;158:513–6. Low-affinity penicillin-binding protein associated with beta-lactam resistance in Staphylococcus aureus.
  140. Matsuhashi  M, Song  MD, Ishino  F, Wachi  M, Doi  M, Inoue  M, J Bacteriol. 1986;167:975–80. Molecular cloning of the gene of a penicillin-binding protein supposed to cause high resistance to beta-lactam antibiotics in Staphylococcus aureus.PubMedGoogle Scholar
  141. Song  MD, Maesaki  S, Wachi  M, Takahashi  T, Doi  M, Ishino  F, Primary structure and origin of the gene encoding the beta-lactam-inducible penicillin-binding protein responsible for methicillin-resistance in Staphylococcus aureus. In: Actor P, Daneo-Moore L, Higgins ML, Salton MRJ, Shockman GD, editors. Antibiotic inhibition of bacterial cell surface assembly and function. Washington, DC: American Society for Microbiology, 1988;352-9.
  142. Song  MD, Wachi  M, Doi  M, Ishino  F, Matsuhashi  M. FEBS Lett. 1987;221:167–71. Evolution of an inducible penicillin-target protein in methicillin-resistant Staphylococcus aureus by gene fusion. DOIPubMedGoogle Scholar
  143. Musser  JM, Selander  RK. Genetic analysis of natural populations of Staphylococcus aureus. In: Novick R, Skurray RA, editors. Molecular biology of the staphylococci. New York: VCH Publishers, 1990:59-67.
  144. Musser  JM, Kapur  V. J Clin Microbiol. 1992;30:2058–63. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination.PubMedGoogle Scholar
  145. Kreiswirth  B, Kornblum  J, Arbeit  RD, Eisner  W, Maslow  JN, McGeer  A, Science. 1993;259:227–30. Evidence for a clonal origin of methicillin resistance in Staphylococcus aureus. DOIPubMedGoogle Scholar
  146. Boerlin  P, Peter  O, Bretz  AG, Postic  D, Baranton  G, Piffaretti  J-C. Infect Immun. 1992;60:1677–83. Population genetic analysis of Borrelia burgdorferi isolates by multilocus enzyme electrophoresis.PubMedGoogle Scholar
  147. Baranton  G, Postic  D, Saint Girons  I, Boerlin  P, Piffaretti  J-C, Assous  M, Int J Syst Bacteriol. 1992;42:378–83. Delineation of Borrelia burgdorferi sensu stricto, Borrelia garinii sp. nov., and group VS461 associated with Lyme borreliosis.PubMedGoogle Scholar
  148. Marconi  RT, Garon  CF. J Gen Microbiol. 1992;138:533–6. Identification of a third genomic group of Borrelia burgdorferi through signature nucleotide analysis and 16S rRNA sequence determination.PubMedGoogle Scholar
  149. Welsh  J, Pretzman  C, Postic  D, Saint Girons  I, Baranton  G, McClelland  M. Int J Syst Bacteriol. 1992;42:370–7.Genomic fingerprinting by arbitrarily primed polymerase chain reaction resolves Borrelia burgdorferi into three distinct phyletic groups.PubMedGoogle Scholar
  150. van Dam  AP, Kuiper  H, Vos  K, Clin Infect Dis. 1993;17:708–17. Different genospecies of Borrelia burgdorferi are associated with distinct clinical manifestations.PubMedGoogle Scholar
  151. Balmelli  T, Piffaretti  J-C. Association between different clinical manifestations of Lyme disease and different species of Borrelia burgdorferi sensu lato. Res Microbiol. 1995;146:329–40. DOIPubMedGoogle Scholar
  152. Assous  MV, Postic  D, Paul  G, Nevot  P, Baranton  G. Western blot analysis of sera from Lyme borreliosis patients according to the genomic species of the Borrelia strains used as antigens. Eur J Clin Microbiol Infect Dis. 1993;12:261–8. DOIPubMedGoogle Scholar
  153. Wachsmuth  IK, Evins  GM, Fields  PI, J Infect Dis. 1993;167:621–6. The molecular epidemiology of cholera in Latin America.PubMedGoogle Scholar
  154. Wachsmuth  K, Olsvik  O, Evins  GM, Popovic  T. Molecular epidemiology of cholera. In: Wachsmuth IK, Blake PA, Olsvik O, editor. Vibrio cholerae and cholera: molecular to global perspectives. Washington, DC: American Society for Microbiology, 1994;357-70.
  155. Faruque  SM, Abdul Alim  ARM, Rahman  MM, Siddique  AK, Sack  RB, Albert  MJ. J Clin Microbiol. 1993;31:2513–6. Clonal relationships among classical Vibrio cholerae O1 strains isolated between 1961 and 1992 in Bangladesh.PubMedGoogle Scholar
  156. Musser  JM, Kroll  JS, Granoff  DM, Global genetic structure and molecular epidemiology of encapsulated Haemophilus influenzae. Rev Infect Dis. 1990;12:75–111.PubMedGoogle Scholar
  157. Piffaretti  JC, Kressebuch  H, Aeschbacher  M, Bille  J, Bannerman  E, Musser  JM, Proc Natl Acad Sci U S A. 1989;86:3818–22. Genetic characterization of clones of the bacterium Listeria monocytogenes causing epidemic disease. DOIPubMedGoogle Scholar
  158. Bibb  WF, Gellin  BG, Weaver  R, Schwartz  B, Plikaytis  BD, Reeves  MW, Appl Environ Microbiol. 1990;56:2133–41. Analysis of clinical and food-borne isolates of Listeria monocytogenes in the United States by multilocus enzyme electrophoresis and application of the method to epidemiologic investigations.PubMedGoogle Scholar
  159. Selander  RK, Smith  NH. Molecular population genetics of Salmonella. Rev Med Microbiol. 1990;1:219–28.
  160. Selander  RK, Li  J, Boyd  EF, Wang  F-S, Nelson  K. DNA sequence analysis of the genetic structure of populations of Salmonella enterica and Escherichia coli. In: Priest FG, Ramos-Cormenzana A, Tindall BJ, editors. Bacterial diversity and systematics. New York: Plenum, 1994;17-49.
  161. Selander  RK, Beltran  P, Smith  NH, Evolutionary genetic relationships of clones of Salmonella serovars causing human typhoid and other enteric fevers. J Bacteriol. 1990;58:1891–901.
  162. Selander  RK, Musser  JM. Population genetics of bacterial pathogenesis. In: Clark V, Iglewski B, editor. Molecular basis of bacterial pathogenesis. San Diego: Academic Press, 1990;11-36.
  163. Fleischmann  RD, Adams  MD, White  O, Rd. Science. 1995;269:496–512. Whole-genome random sequencing and assembly of Haemophilus influenzae DOIPubMedGoogle Scholar
  164. Fraser  CM, Gocayne  JD, White  O, Science. 1995;270:397–403. The minimal gene complement of Mycoplasma genitalium. DOIPubMedGoogle Scholar

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