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Volume 20, Number 1—January 2014
Dispatch

Dobrava-Belgrade Virus in Apodemus flavicollis and A. uralensis Mice, Turkey

I. Mehmet Ali Oktem, Yavuz Uyar, Ender Dincer, Aysegul Gozalan, Mathias Schlegel, Cahit Babur, Bekir Celebi, Mustafa Sozen, Ahmet Karatas, Nuri Kaan Ozkazanc, Ferhat Matur, Gulay Korukluoglu, Rainer G. Ulrich, Mustafa Ertek, and Aykut OzkulComments to Author 
Author affiliations: Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey (I.M.A. Oktem); Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey (Y. Uyar); Ankara University, Ankara, Turkey (E. Dincer); Refik Saydam National Public Health Agency, Ankara (A. Gozalan, C. Babur, B. Celebi, G. Korukluoglu, M. Ertek); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (M. Schlegel, R.G. Ulrich); Karaelmas University, Zonguldak, Turkey (M. Sozen, F. Matur); Nigde University, Nigde, Turkey (A. Karatas); Bartin University, Bartin, Turkey (N.K. Ozkazanc); Faculty of Veterinary Medicine, Ankara University, Ankara (A. Ozkul)

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Figure 2

Bayesian phylogenetic tree, based on an alignment of 450-nt long region of the small segment from various Dobrava-Belgrade virus lineages and other Murinae-associated hantaviruses. Posterior probabilities for Bayesian analysis are given under the branches and bootstrap values above the branches. Values lower than <0.7% and <70% are not shown. The sequences were aligned with ClustalW included in the BioEdit software package version 2.1 (http://www.mbio.ncsu.edu/bioedit/page2.html). The phyl

Figure 2. . Bayesian phylogenetic tree, based on an alignment of 450-nt long region of the small segment from various Dobrava-Belgrade virus lineages and other Murinae-associated hantaviruses. Posterior probabilities for Bayesian analysis are given under the branches and bootstrap values above the branches. Values lower than <0.7% and <70% are not shown. The sequences were aligned with ClustalW included in the BioEdit software package version 2.1 (http://www.mbio.ncsu.edu/bioedit/page2.html). The phylogenetic analyses were performed by using MrBayes 3.1.2 with Bayesian Metropolis-Hastings Markov Chain Monte Carlo (MCMC) tree-sampling methods based on 2 MCMC runs consisting of 4 chains of 2,000,000 with a burn-in of 25% and second by maximum-likelihood bootstrap analysis with 1,000 pseudoreplicates using MEGA5 (www.megasoftware.net). The Hasegawa-Kishino-Yano model with a discrete gamma distribution, to model evolutionary rate differences among sites (2 categories [+G, parameter = 0.8874]) according to jModeltest (http://code.google.com/p/jmodeltest2/) was used. Af, Apodemus flavicollis; Au, A. uralensis; Ap, A. ponticus; Hu, human; Aa, A. agrarius; SNGV, Sangassou virus; HTNV, Hantaan virus; THAIV, Thailand virus; SEOV, Seoul virus; SK, Slovakia; SLO, Slovenia; GR, Greece; TR, Turkey; RU, Russia; EE, Estonia (island Saaremaa); DE, Germany; HR, Croatia. Scale bar indicates number of nucleotide substitutions per site.

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Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
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