Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 20, Number 1—January 2014
Dispatch

Dobrava-Belgrade Virus in Apodemus flavicollis and A. uralensis Mice, Turkey

I. Mehmet Ali Oktem, Yavuz Uyar, Ender Dincer, Aysegul Gozalan, Mathias Schlegel, Cahit Babur, Bekir Celebi, Mustafa Sozen, Ahmet Karatas, Nuri Kaan Ozkazanc, Ferhat Matur, Gulay Korukluoglu, Rainer G. Ulrich, Mustafa Ertek, and Aykut OzkulComments to Author 
Author affiliations: Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey (I.M.A. Oktem); Cerrahpasa Faculty of Medicine, Istanbul University, Istanbul, Turkey (Y. Uyar); Ankara University, Ankara, Turkey (E. Dincer); Refik Saydam National Public Health Agency, Ankara (A. Gozalan, C. Babur, B. Celebi, G. Korukluoglu, M. Ertek); Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany (M. Schlegel, R.G. Ulrich); Karaelmas University, Zonguldak, Turkey (M. Sozen, F. Matur); Nigde University, Nigde, Turkey (A. Karatas); Bartin University, Bartin, Turkey (N.K. Ozkazanc); Faculty of Veterinary Medicine, Ankara University, Ankara (A. Ozkul)

Main Article

Table 2

Serologic reactivity of 23 DOBV RT-qPCR–positive rodents by ELISA and IFA, Turkey, 2009*

Animal no. Species (cyt b)† Location/district‡ Hantavirus serology
ELISA
IFA
IgM IgG IgG
94-09 Apodemus uralensis Akbas Neg Neg Neg
95-09 A. uralensis Akbas Neg Neg NS
121-09 A. flavicollis Akbas Neg Neg Neg
123-09 A. flavicollis Akbas Neg Neg Pos
124-09 A. uralensis Akbas Neg Neg BL
130-09 A. uralensis Akbas Neg Neg Neg
139-09 A. flavicollis Akbas Neg Pos Pos
142-09 A. flavicollis Akbas Neg Pos Neg
151-09 A. uralensis Bogaz Neg Neg Neg
162-09 A. uralensis Bogaz Neg Neg Neg
169-09 A. flavicollis Bogaz Neg Pos Neg
177-09 A. uralensis Bogaz Neg Neg Neg
178-09 A. uralensis Guzelcehisar Neg Neg NS
180-09 A. uralensis Guzelcehisar NS§ NS NS
100-09 A. uralensis Hanyeri Neg Neg Neg
102-09 A. uralensis Hanyeri Neg Neg Neg
106-09 Rattus rattus Hasankadi Neg Pos Neg
25-09 A. flavicollis Hasankadi Pos Neg Neg
78-09 A. flavicollis Kumluca Neg Pos Pos
80-09 A. uralensis Kumluca Neg Pos Pos
81-09 A. flavicollis Kumluca Neg Pos Pos
134-09 A. flavicollis Kumluca Neg Pos Pos
137-09 A. flavicollis Kumluca Neg Neg Pos

*DoBV, Dobrava-Belgrade virus; RT-qPCR, reverse transcription quantitative PCR; IFA, immunofluorescence assay; NS, no serum sample available; BL, borderline.
†Cytochrome b–based genetic species determination following a recently published protocol (9).
‡In alphabetical order.

Main Article

References
  1. Krüger  DH, Schonrich  G, Klempa  B. Human pathogenic hantaviruses and prevention of infection. Hum Vaccin. 2011;7:68593 . DOIPubMedGoogle Scholar
  2. Klempa  B, Avsic-Zupanc  T, Clement  J, Dzagurova  TK, Henttonen  H, Heyman  P, Complex evolution and epidemiology of Dobrava-Belgrade hantavirus: definition of genotypes and their characteristics. Arch Virol. 2013;158:5219 . DOIPubMedGoogle Scholar
  3. Guo  WP, Lin  XD, Wang  W, Tian  JH, Cong  ML, Zhang  HL, Phylogeny and origins of hantaviruses harbored by bats, insectivores, and rodents. PLoS Pathog. 2013;9:e1003159 . DOIPubMedGoogle Scholar
  4. Laakkonen  J, Kallio-Kokko  H, Öktem  MA, Blasdell  K, Plyusnina  A, Niemimaa  J, Serological survey for viral pathogens in Turkish rodents. J Wildl Dis. 2006;42:6726 . DOIPubMedGoogle Scholar
  5. Kavukcu  S, Turkmen  M, Salman  S, Soylu  A, Camsari  T. What is the risk of nephropathy associated with hantavirus in Aegean region? J Turkish Nephrol Assoc. 1997;3–4:131.
  6. Ertek  M, Buzgan  T. An outbreak caused by hantavirus in the Black Sea region of Turkey, January–May 2009. Euro Surveill. 2009;14:19214 .PubMedGoogle Scholar
  7. Oktem  MA. Hantavirus and tick-borne encephalitis infections [in Turkish]. Ankem Derg. 2009;23(Suppl 2):2458.
  8. Oncul  O, Atalay  Y, Onem  Y, Turhan  V, Acar  A, Uyar  Y, Hantavirus infection in Istanbul, Turkey. Emerg Infect Dis. 2011;17:3034 . DOIPubMedGoogle Scholar
  9. Schlegel  M, Ali  HS, Stieger  N, Groschup  MH, Wolf  R, Ulrich  RG. Molecular identification of small mammal species using novel cytochrome b gene–derived degenerated primers. Biochem Genet. 2012;50:4407. DOIPubMedGoogle Scholar
  10. Heyman  P, Cochez  C, Korukluoglu  G, Gozalan  A, Uyar  Y, Lundkvist  A. Bridging continents; Hantaviruses of Europe and Asia Minora; bridging continents. Hantaviruses of Europe and Asia Minor. Turk Hij Den Biyol Derg. 2011;68:41–8.
  11. Weidmann  M, Schmidt  P, Vackova  M, Krivanec  K, Munclinger  P, Hufert  FT. Identification of genetic evidence for Dobrava virus spillover in rodents by nested reverse transcription (RT)-PCR and TaqMan RT-PCR. J Clin Microbiol. 2005;43:80812. DOIPubMedGoogle Scholar
  12. Schlegel  M, Klempa  B, Auste  B, Bemmann  M, Schmidt-Chanasit  J, Buchner  T, Dobrava-Belgrade virus spillover infections, Germany. Emerg Infect Dis. 2009;15:201720. DOIPubMedGoogle Scholar
  13. Heroldová  M, Pejcoch  M, Bryja  J, Janova  E, Suchomel  J, Tkadlec  E. Tula virus in populations of small terrestrial mammals in a rural landscape. Vector Borne Zoonotic Dis. 2010;10:599603. DOIPubMedGoogle Scholar
  14. Hjelle  B, Yates  T. Modeling hantavirus maintenance and transmission in rodent communities. Curr Top Microbiol Immunol. 2001;256:7790. DOIPubMedGoogle Scholar
  15. Schlegel  M, Kindler  E, Essbauer  SS, Wolf  R, Thiel  J, Groschup  MH, Tula virus infections in the Eurasian water vole in Central Europe. Vector Borne Zoonotic Dis. 2012;12:50313. DOIPubMedGoogle Scholar

Main Article

Page created: January 03, 2014
Page updated: January 03, 2014
Page reviewed: January 03, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external