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Volume 20, Number 1—January 2014
Letter

Endemicity of Opisthorchis viverrini Liver Flukes, Vietnam, 2011–2012

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To the Editor: Fishborne zoonotic trematodes are highly prevalent in many Asian communities (1,2). Although presence of the liver fluke Clonorchis sinensis is well documented in Vietnam (3), evidence of the presence of the more common liver fluke of Southeast Asia, Opisthorchis viverrini, is only circumstantial. Surveys of human fecal samples have frequently reported O. viverrini fluke eggs in humans in southern and central Vietnam (4); however, identifications based on fecal eggs are notoriously unreliable for differentiating species of liver and intestinal flukes (5). The few reports of surgical recovery of adult O. viverrini flukes from humans do not eliminate the possibility of infection having been acquired during travel in neighboring fluke-endemic countries.

Metaceraria from fish in the Mekong Delta have been tentatively identified as Opisthorchis spp., but this identity has not been confirmed (6). Specific identification is necessary for an understanding of the liver fluke diversity in Vietnam, especially because O. lobatus flukes, a related species that infects ducks, have been reported from nearby Laos (7).

To clarify the status of fishborne liver flukes in Vietnam, during 2011–2012, we conducted a survey for liver fluke metacercariae in fish from Phu Yen Province. We selected this province because the local populations have a strong preference for raw fish and because previous surveys of human fecal samples conducted there indicated high prevalence of fishborne parasites (4). We chose to investigate metacercariae in fish to avoid the uncertainty of identifications based on fecal eggs and because of the availability of recent molecular methods for species identification of Opisthorchis fluke metacercariae (7).

Fish were collected from Tuy Hoa City and from the districts of Hoa Xuan Dong, Tuy An, and Song Hinh; these 3 districts are areas of large aquaculture production of freshwater fish. Fresh fish from ponds, rice fields, rivers, and swamps were purchased at local markets from April 2011 through March 2012. The fish sellers provided information about the source of the fish (e.g., type of water body). Fish were transported live with mechanical aeration to the Research Institute for Aquaculture No. 3 in Nha Trang, where they were examined for metacercariae by use of whole individual fish pepsin digestion (8).

Recovered metacercariae were examined microscopically, and those identified morphologically as Opisthorchis spp. flukes (9) were isolated. A subset of these metacercariae were fixed in 70% alcohol and examined by PCR and sequence analysis of the CO1 gene (7) at the Department of Helminthology, Mahidol University, Bangkok. For the purpose of obtaining adult worms, 3 hamsters were inoculated with the Opisthorchis metacercariae (15, 30, or 45 metacercariae/hamster). The adult worms were recovered from the infected hamsters 25–30 days after infection and were fixed and stained for morphologic determination of species (10).

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Thumbnail of Morphologic appearance of different stages of Opisthorchis viverrinia flukes. A) Encysted metacercariae. Scale bar indicates 30 μ. . B) Metacercariae released from cyst. Scale bar indicates 30 μ. C) Carmine-stained adult worm from experimentally infected hamster. Scale bar indicates 1 mm.

Figure. Morphologic appearance of different stages of Opisthorchis viverrini flukes. A) Encysted metacercariae. Scale bar indicates 30 μ. B) Metacercariae released from cyst. Scale bar indicates 30 μ. C) Carmine-stained adult worm...

A total of 4 fish species were infected with O. viverrini metacercariae (Technical Appendix Table 1). Metacercariae prevalence was highest (28.1%) among crucian carp (Carasius auratus). Specific identification was confirmed by morphologic appearance of adult worms recovered from hamsters (Figure) and PCR and sequence analysis of the partial metacercarial CO1 gene, amplified by CO1-OV-Hap-F&R primers (7). Infected fish originated predominantly from so-called wild water (i.e., swamps, rice fields, rivers). The prevalence of O. viverrini metacercariae in crucian carp varied seasonally (Technical Appendix Table 2).

Crucian carp are cultured in some countries but not in Vietnam. However, the high prevalence and mean intensity of O. viverrini metacercariae (28.3 metacercariae/fish) is of public health concern because wild species such as crucian carp are often eaten raw, marinated, or lightly cooked. In contrast, infected barb (Puntius brevis) and rasbora (Rasbora spp.) fish (Technical Appendix Table 1) are not eaten raw. However, barb fish are invasive in farm fish ponds and can persist as a self-recruiting species; the presence of barb is an indication that pond management is insufficient to prevent invasive species of fish. Furthermore, barb fish are often fed to farm cats, which are major reservoir hosts for fishborne liver and intestinal trematodes. Infections (prevalence 8.3%) in snakehead fish (Channa spp.) also represent a food safety risk, because snakehead fish are cultured in Vietnam and are sometimes eaten raw or inadequately cooked. In addition to O. viverrini flukes, metacercariae of the zoonotic intestinal flukes Centrocestus formosanus, Haplorchis taichui, and H. yokogawi were recovered from snakehead and barb fish (Technical Appendix Table 1), all of which are common throughout Southeast Asia (1).

The results of this study demonstrate that the human liver fluke O. viverrini is endemic to Vietnam and that it is being naturally transmitted to fish species that are often consumed raw or inadequately cooked. For determination of the prevalence, distribution, and epidemiology of O. viverrini flukes in fish, humans, and reservoir hosts (e.g., cats and dogs), these results need to be extended, especially because aquaculture is a growing industry in Vietnam.

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Acknowledgments

We thank Henry Madsen for his expert ecologic and malacologic advice, Jesper Clausen for his valuable assistance, PhanThi Van and Anders Dalsgaard for their strong support and advice, and the staff of the Research Institute for Aquaculture No.3 for their support and contributions in the field and laboratory.

This study is part of the research capacity building project “Fishborne Zoonotic Parasites in Vietnam” (www.fibozopa2.ria1.org; project no. 91140), financially supported by the Danish International Development Assistance.

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Jitra Waikagul, Bui Ngoc Thanh, Dung Thi Vo, Duy Nhat Nguyen, K. Darwin MurrellComments to Author , and Vo The Dung

Author affiliations: Research Institute for Aquaculture No. 3, NhaTrang, Vietnam (V.T. Dung, D.T. Vo, D.N. Nguyen); Mahidol University, Bangkok, Thailand (J. Waikagul); Research Institute for Aquaculture No. 1, Bac Ninh, Vietnam (B.N. Thanh); University of Copenhagen, Copenhagen, Denmark (K.D. Murrell)

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References

  1. Chai  JY, Murrell  KD, Lymbery  AJ. Fish-borne parasitic zoonoses: status and issues. Int J Parasitol. 2005;35:123354 . DOIPubMed
  2. Sithithaworn  P, Andrews  RH, Nguyen  VD, Wongsaroj  T, Sinuon  M, Odermatt  P, The current status of opisthorchiasis and clonorchiasis in the Mekong Basin. Parasitol Int. 2012;61:106. DOIPubMed
  3. Trung Dung  D, Van De  N, Waikagul  J, Dalsgaard  A, Chai  JY, Sohn  WM, Fishborne zoonotic intestinal trematodes in Vietnam. Emerg Infect Dis. 2007;13:182833. DOIPubMed
  4. De  NV, Murrell  KD, Cong le  D, Cam  PD, Chau le  V, Toan  ND, The food-borne trematode zoonoses of Vietnam. Southeast Asian J Trop Med Public Health. 2003;34(Suppl 1):1234.PubMed
  5. Ditrich  O, Giboda  M, Sterba  J. Species determination of eggs of opisthorchiid and heterophyid flukes using scanning electron microscopy. Angew Parasitol. 1990;31:39 .PubMed
  6. Thu  ND, Dalsgaard  A, Loan  LT, Murrell  KD. Survey for zoonotic liver and intestinal trematode metacercariae in cultured and wild fish in An Giang Province, Vietnam. Korean J Parasitol. 2007;45:4554. DOIPubMed
  7. Thaenkham  U, Nuamtnong  S, Vonghachack  Y, Yoonuan  T, Sanguankiat  S, Dekumyoy  P, Discovery of Opisthorchis lobatus (Trematoda: Opisthorchiidae): new recorded species of small liver flukes in the greater Mekong sub-region. J Parasitol. 2011;97:11528. DOIPubMed
  8. Vo  DT, Murrell  D, Dalsgaard  A, Bristow  G, Nguyen  DH, Bui  TN, Prevalence of zoonotic metacercariae in two species of grouper, Epinephelus coioides and Epinephelus bleekeri, and flathead mullet, Mugil cephalus, in Viet Nam. Korean J Parasitol. 2008;46:7782. DOIPubMed
  9. Scholz  T, Ditrich  O, Giboda  M. Differential diagnosis of opisthorchiid and heterophyid metacercariae (Trematoda) infecting fish of cyprinid fish from Nam Ngum Dam. Southeast Asian J Trop Med Public Health. 1991;22:1713.PubMed
  10. Scholz  T. Family Opisthorchiidae Looss, 1899. In: Bray RA, Gibson DI, Jones A, editors. Keys to the trematoda, volume 3. London: CAB International and Natural History Museum; 2008. p. 9–49.

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Cite This Article

DOI: 10.3201/eid2001.130168

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Table of Contents – Volume 20, Number 1—January 2014

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K. Darwin Murrell, Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USAK. Darwin Murrell, Department of Preventive Medicine and Biometrics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Rd, Bethesda, MD 20814, USA

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Page created: December 18, 2013
Page updated: December 18, 2013
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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