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Volume 20, Number 1—January 2014
Letter

Corynebacterium ulcerans in Ferrets

Robert P. Marini, Pamela K. Cassiday, Jaime Venezia, Zeli Shen, Ellen M. Buckley, Yaicha Peters, Nancy Taylor, Floyd E. Dewhirst, Maria L. Tondella, and James G. FoxComments to Author 
Author affiliations: Massachusetts Institute of Technology, Cambridge, Massachusetts, USA (R.P. Marini, J. Venezia, Z. Shen, E.M. Buckley, Y. Peters, N. Taylor, J.G. Fox); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (P.K. Cassiday, M.L. Tondella); The Forsyth Institute, Cambridge (F.E. Dewhirst); Harvard School of Dental Medicine, Boston, Massachusetts, USA (F.E. Dewhirst)

Main Article

Table

Identification of Corynebacterium ulcerans strains isolated from ferrets*

MIT accession no. CDC API code CDC interpretation (confidence limit, %) Isolate source CDC MALDI-TOF-MS (score)† 16S rRNA 
(confidence limit, %), 
GenBank accession no. rpoB 
(confidence limit, %), 
GenBank accession no.
07–3331 0101326‡ C. ulcerans (87.3), C. pseudotuberculosis (12.5) Oropharynx C. ulcerans (2.13) C. ulcerans (99.5) KF564646 C. ulcerans (99.5) KF539859
08–0584 0111326 C. ulcerans (99.7) Cephalic implant C. ulcerans (2.35) C. ulcerans (99.5) KF564647 C. ulcerans (99.5) KF539860
07–3276 0111326 C. ulcerans (99.7) Cephalic implant C. ulcerans (2.24) C. ulcerans (99.7) KF564645 C. ulcerans (99.5) KF539858

*Identification was performed by use of the API Coryne strip system (bioMérieux, Durham, NC, USA), MALDI-TOF-MS (matrix-assisted laser desorption–ionization time-of-flight mass spectroscopy) (Bruker Daltonics; Fremont, CA, USA), and gene sequencing. Percentages after the API identification refer to confidence limits. Percentages after the gene sequencing results refer to percentage identities with a reference strain (4). MIT, Massachusetts Institute of Technology; CDC, Centers for Disease Control and Prevention.
†2.000–2.299, secure genus identification, probable species identification; 2.300–3.000, highly probable species identification (4).
‡The API code generated at MIT was 0101320, interpreted as C. pseudotuberculosis (99.5%).

Main Article

Page created: January 03, 2014
Page updated: January 03, 2014
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