Fecal samples were collected from outpatients and patients with symptoms of enteritis who were admitted for <72 hours to the Sint-Lucas Hospital in Ghent, Belgium, during 2008–2013. All age groups were represented. We examined the fecal samples macroscopically for consistency and presence of blood and mucus and microscopically for leukocytes and cultured them for all common bacterial pathogens. Feces of patients <2 years of age were also tested for rotavirus and enteric adenovirus, and those of adult patients with loose or mucous-containing stools were also tested for toxigenic Clostridium difficile. Samples were tested for parasites on clinical demand only.

We cultured for Arcobacter using an isolation method for fecal samples carried out in veterinary medicine that was previously validated for use on human feces (11): 1 g of feces was inoculated into a liquid selective broth (24 g/L Arcobacter broth with 50 mL lysed, defibrinated horse blood and an antimicrobial supplement of 16 mg/L cefoperazone, 10 mg/L amphotericin B, 100 mg/L 5-fluorouracil, 64 mg/L trimethoprim, and 32 mg/L novobiocine [Oxoid, Cambridge, United Kingdom]) and incubated for 24 h at 25°C in a microaerobic atmosphere of 6% O_2, 7% CO_2, 7% H₂, and 80% N₂ (Advanced Instruments, Inc., Norwood, MA, USA). Then, 40 μL of enriched broth was plated onto a solid Arcobacter-selective medium with the same composition as the broth but with the addition of 12 g/L agar technical no. 3 (Oxoid) and without the horse blood. The plates were incubated for 72 h at 25°C in a microaerobic atmosphere and examined daily. Arcobacter colonies were detected by screening the selective transparent agar as described with Henry transillumination microscopy for bluish colonies (11). Definitive identification was performed by a genus- and species-specific multiplex PCR, and subsequently, by amplification fragment length polymorphism (12,13).

Of the 8,994 eligible samples received during the study period, 6,774 (75.31%) samples were cultured for Arcobacter; 2,220 samples (24.68%) were excluded because of insufficient samples size. Of samples from the study population, Campylobacter spp. were isolated in 380 (5.61%), Salmonella spp. in 138 (2.04%), toxigenic C. difficile in 109 (1.61%), Aeromonas spp. in 16 (0.24%), and Yersinia enterocolitica in 13 (0.19%). Other gastrointestinal pathogens isolated in lower numbers included Shigella spp. (9 samples, 0.13%) and Plesiomonas spp. (3 samples, 0.05%).

Arcobacter spp. were isolated in samples from 89 patients (1.31%), ranking members of this genus as the fourth most commonly isolated pathogen group in the study. This ranking is comparable to those of other reports, although a higher isolation rate was obtained during this study (14,15). The distribution frequency of pathogens and of A. butzleri versus A. cryaerophilus, 49 (0.72%) and 38 (0.56%) isolates, respectively, is shown in Table 1. A. skirrowii was not recovered, but the first 2 known isolates of A. thereius (0.03%) from humans were documented. In the excluded population (n = 2,220), comparable recovery rates of Campylobacter spp. (6.53%), Salmonella spp. (2.88%) and C. difficile (2.07%) were observed. In 6 patients whose samples were positive for Arcobacter, a second pathogen was isolated: 1 patient was positive for Salmonella spp., samples from 2 patients showed Campylobacter spp. and A. butzleri, and toxigenic C. difficile was detected in 2 patients who tested positive for A. butzleri and in 1 patient who tested positive for A. cryaerophilus.

We collected retrospective data about age, hospital stay, presence of diarrhea, clinical diagnosis of enteritis, colonoscopy results, and underlying disease for the positive-culture patient group. We assessed differences between study populations and results using t-test and Χ² statistical methods as appropriate.

Figure

Figure. Age distribution of study population for detection of Arcobacter spp. in patients with acute enteritis, 2008–2013, Belgium. Black bars indicate percentage of age group included, white bars indicate percentage of patients...

The mean age of the included population (29 years) differed significantly (p<0.01) from the excluded group (22 years), because of an excess of infants from whom sample material was insufficient in the latter group. The positive-culture patient group had a mean age of 42 years and demonstrated a significantly older age distribution pattern (p<0.01) (Figure). The consistency of fecal samples was not predictive (p = 0.62) for the presence of Arcobacter. Within the Arcobacter-positive group, the mean age of patients whose specimens shed A. butzleri (49 years) was significantly higher (15 years, p<0.01) than that of patients whose specimens shed A. cryaerophilus (34 years). Presence of A. butzleri in patients admitted to the hospital and in outpatients was not significantly linked with underlying disease (p = 0.12). A. cryaerophilus was more frequently observed in outpatients who had uncomplicated gastroenteritis than in hospitalized patients with coexisting conditions (p<0.001) (Table 2).

Arcobacter species were the fourth most common pathogen group isolated from fecal samples from persons with acute enteric disease. The high isolation rate could possibly be explained by the inclusion of an outpatient population, local indications for sampling, and the use of an enrichment culture method. The Arcobacter-positive patients tended to belong to older age groups. No notable association of A. butzleri enteritis with coexisting conditions was observed. The feasibility of selective culturing Arcobacter from fecal material in a routine microbiology hospital laboratory was confirmed, but the slow turnaround times for culture results show a need for optimization of methods. Arcobacter species should be considered and tested for in cases of diarrheal disease.