Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 20, Number 12—December 2014
Dispatch

Mycobacterium Species Related to M. leprae and M. lepromatosis from Cows with Bovine Nodular Thelitis

Didier PinComments to Author , Véronique Guérin-Faublée, Virginie Garreau, Franck Breysse, Oana Dumitrescu, Jean-Pierre Flandrois, and Gerard Lina
Author affiliations: VetAgro Sup Campus Vétérinaire de Lyon, Marcy l’Étoile, France (D. Pin, V. Guérin-Faublée); Clinique Vétérinaire, Saint Bénigne, France (V. Garreau); Centre Hospitalier Lyon Sud, Pierre Bénite, France (F. Breysse); Université Lyon 1, Lyon, France (O. Dumitrescu, J.-P. Flandrois, G. Lina)

Main Article

Table

Primers used for PCR detection of Mycobacterium species, Jura, France

Gene*
Sequence (5′→3′)†
16S rRNA gene (6) F-TCAAAKgAATTgACgggggC
R-ggTTACCTTgTTACgACTT
hsp65 (5) F-ACCAACgATggTgTgTCCAT
R-CTTgTCgAACCgCATACCCT
rpoB (designed for this study) 2F-TCAACgggACCgAgCgTgTC
2R-gTgTTgTCCTTCTCCAgCgT
3F-TCAACgggACCgAgCgTgTC
4R-gTCTCgATCgggCACATC
9F-gTgggCACCggCATggAgTT
9R-ATgTTCATCCgTCgCggC
8F-ATGAAgCTgCACCACTTggT
8R-gCCGATTCgTTgCgggACA
sodA (5) F-AgCTTCACCACAgCAAgCACCA
R-TCggCCAgTTCACgACgTTCCA
tuf (5) F-CACgCCgACTACATCAAgAA
R-gAACTgCggACggTAgTTgT
tmRNA (5) F-ggggCTgAAACggTTTCgAC

R-TggAgCTgCCgggAATCgAAC
*hsp65, heat shock protein 65; rpoB, β-subunit of RNA polymerase; sodA, superoxide dismutase A; tuf, elongation factor Tuf; tmRNA, transfer–messenger RNA. 
†F, forward; R, reverse. DNA was amplified by using a LightCycler 2.0 instrument (Roche Molecular Diagnostics, Indianapolis, IN, USA) and a ready-to-use hot start reaction mixture (TAKARA, Tokyo, Japan). For all PCRs, parameters were denaturation 95°C for 30 s; and amplification (45 cycles) at 95°C for 15 s, 65°C for 20 s, and 72°C for 40 s (slope, 2°C/s); melting curve, 0 s at 95°C, 15 s at 75°C, and 0 s at 98°C (slope, 0.05°C/s).

Main Article

References
  1. Elston  D. Nontuberculous mycobacterial skin infections. Am J Clin Dermatol. 2009;10:2815 . DOIPubMed
  2. Thorel  M-F, Morand  M, Fontaine  J-J, Gourreau  J-M. Bovine nodular thelitis: a clinicopathological study of 20 cases. Vet Dermatol. 1990;1:16570. DOI
  3. Yachida  S, Shimizu  K, Hirose  T, Sato  M. Studies on mycobacteria isolated from skin lesion tuberculosis of the bovine udder. Nihon Juigaku Zasshi. 1973;35:357–65.
  4. Rüsch  P, Corboz  L, Ossent  P, Berchtold  M, Ehrensperger  F. Enzootic nodular-ulcerative mamillitis in dairy cows caused by unclassified mycobacteria [in German]. Schweiz Arch Tierheilkd. 1984;126:46778 .PubMed
  5. Mignard  S, Flandrois  J-P. A seven-gene, multilocus, genus-wide approach to the phylogeny of mycobacteria using supertrees. Int J Syst Evol Microbiol. 2008;58:143241. DOIPubMed
  6. Relman  DA. Universal bacterial 16S rDNA amplification and sequencing. In: Persing DH, Smith TF, Tenover FC, White TJ, editors. Diagnostic molecular microbiology: principles and applications. Washington (DC): American Society for Microbiology; 1993. p. 489–95.
  7. Guérin-Faublée  V, Flandrois  J-P, Pichat  C, Boschiroli  ML, Lamy  B. Mycobacterium bourgelatii sp. nov., a rapidly growing, non-chromogenic species isolated from the lymph nodes of cattle. Int J Syst Evol Microbiol. 2013;63:466974. DOIPubMed
  8. Tortoli  E. Phylogeny of the genus Mycobacterium: many doubts, few certainties. Infect Genet Evol. 2012;12:82731 and. DOIPubMed
  9. Malik  R, Hughes  MS, James  G, Martin  P, Wigney  DI, Canfield  PJ, Feline leprosy: two different clinical syndromes. J Feline Med Surg. 2002;4:4359. DOIPubMed
  10. Han  XY, Sizer  KC, Thompson  EJ, Kabanja  J, Li  J, Hu  P, Comparative sequence analysis of Mycobacterium leprae and the new leprosy-causing Mycobacterium lepromatosis. J Bacteriol. 2009;191:606774. DOIPubMed
  11. Han  XY, Seo  Y-H, Sizer  KC, Schoberle  T, May  GS, Spencer  JS, A new Mycobacterium species causing diffuse lepromatous leprosy. Am J Clin Pathol. 2008;130:85664. DOIPubMed
  12. Lindeboom  JA, Bruijnesteijn van Coppenraet  LES, van Soolingen  D, Prins  JM, Kuijper  EJ. Clinical manifestations, diagnosis, and treatment of Mycobacterium haemophilum infections. Clin Microbiol Rev. 2011;24:70117. DOIPubMed
  13. Monot  M, Honoré  N, Garnier  T, Araoz  R, Coppée  J-Y, Lacroix  C, On the origin of leprosy. Science. 2005;308:10402. DOIPubMed
  14. Schuenemann  VJ, Singh  P, Mendum  TA, Krause-Kyora  B, Jäger  G, Bos  KI, Genome-wide comparison of medieval and modern Mycobacterium leprae. Science. 2013;341:17983. DOIPubMed
  15. Cole  ST, Eiglmeier  K, Parkhill  J, James  KD, Thomson  NR, Wheeler  PR, Massive gene decay in the leprosy bacillus. Nature. 2001;409:100711. DOIPubMed

Main Article

Page created: November 19, 2014
Page updated: November 19, 2014
Page reviewed: November 19, 2014
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external