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Volume 20, Number 2—February 2014

Genomic Variability of Monkeypox Virus among Humans, Democratic Republic of the Congo

Jeffrey R. Kugelman1, Sara C. Johnston1, Prime M. Mulembakani, Neville Kisalu, Michael S. Lee, Galina Koroleva, Sarah E. McCarthy, Marie C. Gestole, Nathan D. Wolfe, Joseph N. Fair, Bradley S. Schneider, Linda L. Wright, John Huggins, Chris A. Whitehouse, Emile Okitolonda Wemakoy, Jean Jacques Muyembe-Tamfum, Lisa E. Hensley, Gustavo F. Palacios2Comments to Author , and Anne W. Rimoin2Comments to Author 
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA (J.R. Kugelman, S.C. Johnston, M.S. Lee, G. Koroleva, S.E. McCarthy, M.C. Gestole, J. Huggins, C.A. Whitehouse, G.F. Palacios); Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo (P.M. Mulembakani, E.O. Wemakoy); University of California, Los Angeles, California, USA (N. Kisalu, A.W. Rimoin); Global Viral Forecasting (now known as Metabiota), San Francisco, California, USA (N.D. Wolfe, J.N, Fair, B.S. Schneider); The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA (L.L. Wright); National Institute of Biomedical Research, Kinshasa (J.J. Muyembe-Tamfum); US Food and Drug Administration, Silver Spring, Maryland, USA (L.E. Hensley)

Main Article

Figure 1

Phylogenetic analysis of whole-genome direct sequencing. Evolutionary relationships between sequenced samples and archived monkeypox virus (MPXV) sequences were determined for the central coding region sequence (MPXV nucleotide positions ≈56000–120000). A cladogram representing the topology of an unrooted Bayesian phylogenetic reconstruction is shown for samples identified by sample number and/or GenBank accession number. The Central and Western African clades and the 4 distinct lineages are ind

Figure 1. Phylogenetic analysis of whole-genome direct sequencingEvolutionary relationships between sequenced samples and archived monkeypox virus (MPXV) sequences were determined for the central coding region sequence (MPXV nucleotide positions ≈56000–120000)A cladogram representing the topology of an unrooted Bayesian phylogenetic reconstruction is shown for samples identified by sample number and/or GenBank accession numberThe Central and Western African clades and the 4 distinct lineages are indicatedConfidence values for branching events were computed by Markov chain Monte Carlo convergenceNumbers at nodes represent Bayesian posterior probabilities computed by using MrBayes 3.1.2 (30).

Main Article

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Main Article

1These authors are co–first authors.

2These authors contributed equally to this work.

Page created: January 17, 2014
Page updated: January 17, 2014
Page reviewed: January 17, 2014
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