Genomic Variability of Monkeypox Virus among Humans, Democratic Republic of the Congo
Jeffrey R. Kugelman1
, Sara C. Johnston1
, Prime M. Mulembakani, Neville Kisalu, Michael S. Lee, Galina Koroleva, Sarah E. McCarthy, Marie C. Gestole, Nathan D. Wolfe, Joseph N. Fair, Bradley S. Schneider, Linda L. Wright, John Huggins, Chris A. Whitehouse, Emile Okitolonda Wemakoy, Jean Jacques Muyembe-Tamfum, Lisa E. Hensley, Gustavo F. Palacios2
, and Anne W. Rimoin2
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA (J.R. Kugelman, S.C. Johnston, M.S. Lee, G. Koroleva, S.E. McCarthy, M.C. Gestole, J. Huggins, C.A. Whitehouse, G.F. Palacios); Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo (P.M. Mulembakani, E.O. Wemakoy); University of California, Los Angeles, California, USA (N. Kisalu, A.W. Rimoin); Global Viral Forecasting (now known as Metabiota), San Francisco, California, USA (N.D. Wolfe, J.N, Fair, B.S. Schneider); The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA (L.L. Wright); National Institute of Biomedical Research, Kinshasa (J.J. Muyembe-Tamfum); US Food and Drug Administration, Silver Spring, Maryland, USA (L.E. Hensley)
Figure 2. Truncation of OMCPA) Whole-genome deep sequencing revealed a 625-bp deletion directly upstream of the right ITR (red box), which completely removed MPV-Z-N2R (locus 201) and truncated OMCP (MPV-Z-N3R, locus 202)A Western African clade virus, MPXV-USA_2003_039, is shown for comparison with OMCP and N2R copy numberPCR amplification regions for the large deletion and the STRs (right and left) are indicated by the blue bar below each ITR diagramThe yellow box represents the ITR region, and all open reading frames are identified by their MPXV-COG_2003_358 locus number with genes of interest in parenthesisB) Nucleotide alignment of the large deletion with MPXV-COG_2003_358 reference sequenceThe truncated protein created by the deletion is indicated by the black backgroundITR, inverted terminal repeat; MPXV, monkeypox virus; COG, Congo; STR, short terminal repeat; OMCP, orthopoxvirus major histocompatibility complex class I–like protein.
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