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Volume 20, Number 2—February 2014
Research

Genomic Variability of Monkeypox Virus among Humans, Democratic Republic of the Congo

Jeffrey R. Kugelman1, Sara C. Johnston1, Prime M. Mulembakani, Neville Kisalu, Michael S. Lee, Galina Koroleva, Sarah E. McCarthy, Marie C. Gestole, Nathan D. Wolfe, Joseph N. Fair, Bradley S. Schneider, Linda L. Wright, John Huggins, Chris A. Whitehouse, Emile Okitolonda Wemakoy, Jean Jacques Muyembe-Tamfum, Lisa E. Hensley, Gustavo F. Palacios2Comments to Author , and Anne W. Rimoin2Comments to Author 
Author affiliations: United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Maryland, USA (J.R. Kugelman, S.C. Johnston, M.S. Lee, G. Koroleva, S.E. McCarthy, M.C. Gestole, J. Huggins, C.A. Whitehouse, G.F. Palacios); Kinshasa School of Public Health, Kinshasa, Democratic Republic of the Congo (P.M. Mulembakani, E.O. Wemakoy); University of California, Los Angeles, California, USA (N. Kisalu, A.W. Rimoin); Global Viral Forecasting (now known as Metabiota), San Francisco, California, USA (N.D. Wolfe, J.N, Fair, B.S. Schneider); The Eunice Kennedy Shriver National Institute of Child Health and Human Development, Bethesda, Maryland, USA (L.L. Wright); National Institute of Biomedical Research, Kinshasa (J.J. Muyembe-Tamfum); US Food and Drug Administration, Silver Spring, Maryland, USA (L.E. Hensley)

Main Article

Figure 2

Truncation of OMCP. A) Whole-genome deep sequencing revealed a 625-bp deletion directly upstream of the right ITR (red box), which completely removed MPV-Z-N2R (locus 201) and truncated OMCP (MPV-Z-N3R, locus 202). A Western African clade virus, MPXV-USA_2003_039, is shown for comparison with OMCP and N2R copy number. PCR amplification regions for the large deletion and the STRs (right and left) are indicated by the blue bar below each ITR diagram. The yellow box represents the ITR region, and a

Figure 2. Truncation of OMCPA) Whole-genome deep sequencing revealed a 625-bp deletion directly upstream of the right ITR (red box), which completely removed MPV-Z-N2R (locus 201) and truncated OMCP (MPV-Z-N3R, locus 202)A Western African clade virus, MPXV-USA_2003_039, is shown for comparison with OMCP and N2R copy numberPCR amplification regions for the large deletion and the STRs (right and left) are indicated by the blue bar below each ITR diagramThe yellow box represents the ITR region, and all open reading frames are identified by their MPXV-COG_2003_358 locus number with genes of interest in parenthesisB) Nucleotide alignment of the large deletion with MPXV-COG_2003_358 reference sequenceThe truncated protein created by the deletion is indicated by the black backgroundITR, inverted terminal repeat; MPXV, monkeypox virus; COG, Congo; STR, short terminal repeat; OMCP, orthopoxvirus major histocompatibility complex class I–like protein.

Main Article

1These authors are co–first authors.

2These authors contributed equally to this work.

Page created: January 17, 2014
Page updated: January 17, 2014
Page reviewed: January 17, 2014
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