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Volume 20, Number 2—February 2014
Letter

Infectious Schmallenberg Virus from Bovine Semen, Germany

Claudia Schulz1, Kerstin Wernike1, Martin BeerComments to Author , and Bernd Hoffmann
Author affiliations: Friedrich-Loeffler-Institut, Greifswald-Insel Riems, Germany

Main Article

Figure

Detection of Schmallenberg virus (SBV) RNA and antibodies in serum of 5 of 11 cattle (C) injected with SBV RNA–positive bovine semen (quantification cycle [Cq] values 26.4–36.4). Infectivity was measured by using reverse transcription PCR (Cq), competitive ELISA (% negative control), and serum neutralization test (ND50, serum dilution that caused virus neutralization in 50% of the replicates). RNAemia and seroconversion occurred in C3, injected with pooled semen from bull C (Cq 34.2), and in C5

Figure. . . Detection of Schmallenberg virus (SBV) RNA and antibodies in serum of 5 of 11 cattle (C) injected with SBV RNA–positive bovine semen (quantification cycle [Cq] values 26.4–36.4). Infectivity was measured by using reverse transcription PCR (Cq), competitive ELISA (% negative control), and serum neutralization test (ND50, serum dilution that caused virus neutralization in 50% of the replicates). RNAemia and seroconversion occurred in C3, injected with pooled semen from bull C (Cq 34.2), and in C5 and C9–C11, injected with pooled or single semen straws from bull F (Cq 26.4). The onset of infection in cattle injected with semen from bull F varied between 3 and 5 days postinfection. Black lines indicate virologic and serologic results for cattle that remained uninfected. Dotted lines indicate positive (top) and negative (bottom) cutoff values; whiskers indicate SD of the mean.

Main Article

1These authors contributed equally to this article.

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