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Volume 20, Number 3—March 2014

Anaplasma phagocytophilum Antibodies in Humans, Japan, 2010–2011

Yuko Yoshikawa, Norio OhashiComments to Author , Dongxing Wu, Fumihiko Kawamori, Asaka Ikegaya, Takuya Watanabe, Kazuhito Saitoh, Daisuke Takechi, Yoichi Murakami, Daisuke Shichi, Katsumi Aso1, Shuji Ando, and Gaowa
Author affiliations: University of Shizuoka and Global Center of Excellence Program, Shizuoka City, Japan (Gaowa, Y. Yoshikawa, N. Ohashi, D. Wu,); Shizuoka Institute of Environment and Hygiene, Shizuoka City (F. Kawamori, A. Ikegaya); Seirei Hamamatsu General Hospital, Shizuoka (T. Watanabe, K. Saitoh, D. Takechi); Seirei Mikatagahara General Hospital, Shizuoka (Y. Murakami, D. Shichi); Seirei Numazu Hospital, Shizuoka (K. Aso); National Institute of Infectious Diseases, Tokyo, Japan (S. Ando)

Main Article


Immunofluorescence antibody titers to Anaplasma phagoctophilum in serum from 4 patients with human granulocytic anaplasmosis and reactive rP44 protein species, Japan, 2010–2011*

Patient no. Time after illness onset, d Antigen
A. phagocytophilum propagated in THP-1 cells (rP44 species)
A. phagocytophilum propagated in HL60 cells (rP44 species)
1 1 80 (r60) <20 80 (r18ES) <20
15 160 (r60) <20 160 (r18ES) <20

320 (r60)
20 (r60)

320 (r18ES)
40 (r47E)

3 3 40 80 (r60) <20 20 (r18ES)
7 40 80 (r60) <20 20 (r18ES)
80 (r60)
160 (r60)

40 (r18ES)
4 4 160 (r47E) 40 <20 <20
  15 160 (r47E) 80 <20 <20

*Three recombinant P44 (rP44) protein species (r18ES, r47E, r60) were prepared and either one bound to antibodies in each serum sample from 4 patients in Western blot analyses (online Technical Appendix Figure 4, r18ES represents rP44–18ES immunoreactive outer membrane protein that is known to predominate in A. phagocytophilum cultured in HL60 cells (6). r47E and r60 show rP44–47E and rP44–60 proteins, respectively, that are dominantly transcribed in A. phagocytophilum propagated in THP-1 cells (2).

Main Article

1Current affiliation: Aso Clinic, Numazu, Japan.

Page created: February 20, 2014
Page updated: February 20, 2014
Page reviewed: February 20, 2014
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