Volume 20, Number 5—May 2014
Research
Persistence and Complex Evolution of Fluoroquinolone-Resistant Streptococcus pneumoniae Clone
, Mitchell J. Schwaber, Amos Adler, Samira Masarwa, Rotem Edgar, Shiri Navon-Venezia, David Schwartz, Nurith Porat, Tali Kotlovsky, Nikolay Polivkin, Irina Weinberg, Avraham Lazary, Nissim Ohana, and Ron Dagan
Table 1
Characterization of Streptococcus pneumoniae and quinolone resistance–determining region sequences of type II topoisomerase enzymes GyrA, GyrB, and ParC in a post–acute care facility, Israel, 2006–2011*
| Isolate |
Serotype |
PFGE
type |
MLST |
GyrA | GyrB | ParC | ParE | Etest† | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Missense mutation |
Silent
mutation |
Missense mutation |
Silent
mutation |
Missense mutation |
Silent
mutation |
Missense mutation |
Silent
mutation |
mxf |
ofx |
cip |
Lev |
||||
| 109 | 19F | D | ST156 Spain 9V-3 | S81F | – | – | V381, G384, L386 | S79Y | Q41 | – | – | 6, R | >32, R | >32, R | >32, R |
| 116 | 19F | E | ST156 Spain 9V-3 | S81F | – | – | V381, G384, L386 | S79Y | Q41 | – | – | 6, R | >32, R | >32, R | >32, R |
| 129 | 23F | A2 | ST156 Spain 9V-3 | S81F | – | – | V381, G384, L386 | S79Y | Q41 | – | – | 6, R | >32, R | >32, R | >32, R |
| 182 | 23F | A | ST156 Spain 9V-3 | S81F | – | – | V381, G384, L386 | S79Y | Q41 | – | – | 6, R | >32, R | >32, R | >32, R |
| 190 | 19F | C | ST81 Spain 23F-1 | – | – | – | N461, A472 | K137N | Q41, G128 | I460V | I476 | 0.19, S | 4, I | 1.5, S | 1, S |
| 177 | 19F | C | ND | – | – | – | V381, G384, L386 | K137N | Q41, G128 | A326V, E474K I460V | I476 | 0.19, S | 4, I | 6, I | 1.5, I |
| 200 | 15B‡ | H | ND | – | – | – | – | – | Q41 | I460V | – | 0.125, S | 2, S | 1, S | 0.75, S |
*PFGE, pulsed-field gel electrophoresis; MLST, multilocus sequence typing; ND, not determined; I, intermediate; R, resistance; –, no mutations detected. Boldface font indicates mutations from the literature that conferred fluoroquinolone resistance; underline indicates mutation not recorded in literature; italics font indicates mutation not contributing to fluoroquinolone resistance; regular font indicates silent mutations, all are transition: V381 (gtA-gtG), G384 (ggA-ggG), L386 (ttG-ttA), N461 (aaC aaT), A472 (gcT-gcC), Q41 (caA-caG), G128 (ggC-ggT), I476 (atC-atT)
†Performed on brain-hHeart + blood agar plates and plates were incubated at 35°C CO2.
‡15B is a susceptible reference strain.