Volume 20, Number 5—May 2014
Dispatch
PCR for Detection of Oseltamivir Resistance Mutation in Influenza A(H7N9) Virus
Wei Wang1, Zhigang Song1, Wencai Guan1, Yi Liu, Xiaonan Zhang, Lei Xu, Jianhua Li, Zhenghong Yuan, and Yunwen Hu
Table
Detection of wild-type neuraminidase R292 and mutant 292K influenza A(H7N9) virus in clinical samples from 9 patients in China, by reverse transcription PCR*
| Patient no.† | Outcome | Sample type | Time after oseltamivir treatment started, d | Time after oseltamivir treatment ended, d | 292K:R292 ratio | Viral load, copies/mL‡ |
|---|---|---|---|---|---|---|
| 2 | Died | NPS | 9 | −1 | 100:0 | 1.78 × 105 |
| S | 9 | −1 | 100:0 | 8.76 × 104 | ||
| NPS | 10 | −2 | 100:0 | 6.14 × 104 | ||
| S |
10 |
−2 |
25.7:74.3 |
1.29 × 104 |
||
| 3 | Died | NPS | 13 | 3 | – | ND |
| S | 13 | 3 | – | 3.03 × 103 | ||
| NPS | 16 | 6 | – | ND | ||
| S |
16 |
6 |
100:0 |
1.21 × 104 |
||
| 5 | Died | NPS | 7 | −4 | – | 9.96 × 102 |
| S |
11 |
0 |
– |
ND |
||
| 6 | Recovered | NPS | 16 | −3 | – | ND |
| S |
19 |
0 |
– |
ND |
||
| 7 | Recovered | NPS | 13 | 0 | – | ND |
| S |
13 |
0 |
– |
ND |
||
| 8 | Recovered | NPS | 7 | 0 | – | ND |
| S |
7 |
0 |
100:0 |
1.76 × 104 |
||
| 10 | Recovered | NPS | 11 | −4 | – | ND |
| S |
15 |
0 |
91.7:8.3 |
5.25 × 104 |
||
| 15 | Died | NPS | 20 | 4 | – | ND |
| S |
20 |
4 |
– |
5.62 × 103 |
||
| 17 | Recovered | NPS | 11 | 0 | – | ND |
| S | 11 | 0 | – | 3.06 × 104 |
*NPS, nasopharyngeal swab specimen; S, sputum specimen; –, no ratio because samples had negative results; ND, not detected.
†Patient identification was identical with that used by Hu et al. (3). Patients 15 and 17 are initially reported in the current study.
‡Method used to determine viral load was reported by Hu et al. (3).
1These authors contributed equally to this article.
Page created: April 17, 2014
Page updated: April 17, 2014
Page reviewed: April 17, 2014
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