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Volume 20, Number 5—May 2014

Molecular Characterization of Cryptically Circulating Rabies Virus from Ferret Badgers, Taiwan

Hue-Ying Chiou, Chia-Hung Hsieh, Chian-Ren Jeng, Fang-Tse Chan, Hurng-Yi Wang, and Victor Fei PangComments to Author 
Author affiliations: National Taiwan University, Taipei, Taiwan, Republic of China (H.-Y. Chiou, C.-H. Hsieh, C.-R. Jeng, H.-Y. Wang, V.F. Pang); Council of Agriculture, Executive Yuan, Nantou County, Taiwan, Republic of China (F.-T. Chan)

Main Article

Figure 2

Phylogenetic relationships of 27 rabies virus (RABV) genomes constructed by maximum-likelihood method. Numbers close to the nodes were from 1,000 bootstrap replications. The tree was rooted with RABV from bats and raccoons. Three major groups, Asia, Cosmopolitan, and India, are strongly supported, as indicated (17). There are 4 major lineages within the group from Asia, including previously recognized China I, China II (16), Southeast Asia, and RABV from Taiwan ferret badgers (TWFB). RABVs deriv

Figure 2. Phylogenetic relationships of 27 rabies virus (RABV) genomes constructed by maximum-likelihood methodNumbers close to the nodes were from 1,000 bootstrap replicationsThe tree was rooted with RABV from bats and raccoonsThree major groups, Asia, Cosmopolitan, and India, are strongly supported, as indicated (17)There are 4 major lineages within the group from Asia, including previously recognized China I, China II (16), Southeast Asia, and RABV from Taiwan ferret badgers (TWFB)RABVs derived from Chinese ferret badgers (CNFB) are clustered with China I, indicating that RABVs of TWFB and CNFB are of independent originScale bar indicates nucleotide substitutions per site.

Main Article

  1. Jackson  AC. Pathogenesis. In: Jackson AC, Wunner WH, editors. Rabies. London: Elsevier Academic Press; 2007.
  2. World Health Organization. WHO Expert Consultation on Rabies: second report. Geneva. Organization. 2013;•••:1139.
  3. Wandeler  A. Virus infections of non-domestic carnivores: rabies virus. In: Appel MJ, editor. Virus infections of carnivores. Amsterdam: Elsevier Science Publishers; 1987. p. 449–61.
  4. Barnard  BJH. The role played by wildlife in the epizootiology of rabies in South Africa and South-West Africa. Onderstepoort J Vet Res. 1979;46:15563 .PubMed
  5. Smith  GC, Wilkinson  D. Modelling disease spread in a novel host: rabies in the European badger Meles meles. J Appl Ecol. 2002;39:86574. DOI
  6. Wandeler  A, Wachendorfer  G, Forster  U, Krekel  H, Muller  J, Steck  F. Rabies in wild carnivores in central Europe. II. Virological and serological examinations. Zentralbl Veterinarmed B. 1974;21:75764 . DOIPubMed
  7. Liu  Y, Zhang  SF, Wu  XF, Zhao  JH, Hou  YL, Zhang  F, Ferret badger rabies origin and its revisited importance as potential source of rabies transmission in southeast China. BMC Infect Dis. 2010;10:234. DOIPubMed
  8. Zhenyu  G, Zhen  W, Enfu  C, Fan  H, Junfen  L, Yixin  L, Human rabies cluster following badger bites, People’s Republic of China. Emerg Infect Dis. 2007;13:19567. DOIPubMed
  9. Zhang  S, Tang  Q, Wu  XF, Liu  Y, Zhang  F, Rupprecht  CE, Rabies in ferret badgers, southeastern China. Emerg Infect Dis. 2009;15:9469 . DOIPubMed
  10. Lei  YL, Wang  XG, Liu  FM, Chen  XY, Ye  BF, Mei  JH, Complete genome sequencing and analyses of rabies viruses isolated from wild animals (Chinese ferret-badger) in Zhejiang Province [in Chinese]. Zhonghua Liu Xing Bing Xue Za Zhi. 2009;30:8248 .PubMed
  11. Thompson  JD, Higgins  DG, Gibson  TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994;22:467380. DOIPubMed
  12. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:27319. DOIPubMed
  13. Librado  P, Rozas  J. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009;25:14512. DOIPubMed
  14. Tajima  F. Statistical method for testing the neutral mutation hypothesis by DNA polymorphism. Genetics. 1989;123:58595 .PubMed
  15. Fu  YX, Li  WH. Statistical tests of neutrality of mutations. Genetics. 1993;133:693709 .PubMed
  16. He  CQ, Meng  SL, Yan  HY, Ding  NZ, He  HB, Yan  JX, Isolation and identification of a novel rabies virus lineage in China with natural recombinant nucleoprotein gene. PLoS ONE. 2012;7:e49992. DOIPubMed
  17. Bourhy  H, Reynes  JM, Dunham  EJ, Dacheux  L, Larrous  F, Huong  VT, The origin and phylogeography of dog rabies virus. J Gen Virol. 2008;89:267381. DOIPubMed
  18. Guindon  S, Gascuel  O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003;52:696704. DOIPubMed
  19. Posada  D. jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008;25:12536. DOIPubMed
  20. Ronquist  F, Huelsenbeck  JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19:15724. DOIPubMed
  21. Drummond  AJ, Suchard  MA, Xie  D, Rambaut  A. Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol. 2012;29:196973. DOIPubMed
  22. Drummond  AJ, Ho  SY, Phillips  MJ, Rambaut  A. Relaxed phylogenetics and dating with confidence. PLoS Biol. 2006;4:e88. DOIPubMed
  23. Meng  S, Sun  Y, Wu  X, Tang  J, Xu  G, Lei  Y, Evolutionary dynamics of rabies viruses highlights the importance of China rabies transmission in Asia. Virology. 2011;410:4039. DOIPubMed
  24. Kissi  B, Tordo  N, Bourhy  H. Genetic polymorphism in the rabies virus nucleoprotein gene. Virology. 1995;209:52637. DOIPubMed
  25. Mebatsion  T, Weiland  F, Conzelmann  KK. Matrix protein of rabies virus is responsible for the assembly and budding of bullet-shaped particles and interacts with the transmembrane spike glycoprotein G. J Virol. 1999;73:24250 .PubMed
  26. Finke  S, Mueller-Waldeck  R, Conzelmann  KK. Rabies virus matrix protein regulates the balance of virus transcription and replication. J Gen Virol. 2003;84:161321. DOIPubMed
  27. Kassis  R, Larrous  F, Estaquier  J, Bourhy  H. Lyssavirus matrix protein induces apoptosis by a TRAIL-dependent mechanism involving caspase-8 activation. J Virol. 2004;78:654355. DOIPubMed
  28. Lentz  TL, Wilson  PT, Hawrot  E, Speicher  DW. Amino acid sequence similarity between rabies virus glycoprotein and snake venom curaremimetic neurotoxins. Science. 1984;226:8478. DOIPubMed
  29. Poch  O, Blumberg  BM, Bougueleret  L, Tordo  N. Sequence comparison of five polymerases (L proteins) of unsegmented negative-strand RNA viruses: theoretical assignment of functional domains. J Gen Virol. 1990;71:115362. DOIPubMed
  30. Kuzmin  IV, Wu  X, Tordo  N, Rupprecht  CE. Complete genomes of Aravan, Khujand, Irkut and West Caucasian bat viruses, with special attention to the polymerase gene and non-coding regions. Virus Res. 2008;136:8190. DOIPubMed
  31. Zhang  S, Zhao  J, Liu  Y, Fooks  AR, Zhang  F, Hu  R. Characterization of a rabies virus isolate from a ferret badger (Melogale moschata) with unique molecular differences in glycoprotein antigenic site III. Virus Res. 2010;149:14351. DOIPubMed
  32. Gong  W, Jiang  Y, Za  Y, Zeng  Z, Shao  M, Fan  J, Temporal and spatial dynamics of rabies viruses in China and Southeast Asia. Virus Res. 2010;150:1118. DOIPubMed
  33. Saito  M, Oshitani  H, Orbina  JR, Tohma  K, de Guzman  AS, Kamigaki  T, Genetic diversity and geographic distribution of genetically distinct rabies viruses in the Philippines. PLoS Negl Trop Dis. 2013;7:e2144. DOIPubMed
  34. Streicker  DG, Lemey  P, Velasco-Villa  A, Rupprecht  CE. Rates of viral evolution are linked to host geography in bat rabies. PLoS Pathog. 2012;8:e1002720. DOIPubMed
  35. Biek  R, Henderson  JC, Waller  LA, Rupprecht  CE, Real  LA. A high-resolution genetic signature of demographic and spatial expansion in epizootic rabies virus. Proc Natl Acad Sci U S A. 2007;104:79938. DOIPubMed
  36. Faber  M, Faber  ML, Papaneri  A, Bette  M, Weihe  E, Dietzschold  B, A single amino acid change in rabies virus glycoprotein increases virus spread and enhances virus pathogenicity. J Virol. 2005;79:141418. DOIPubMed
  37. Faber  M, Li  J, Kean  RB, Hooper  DC, Alugupalli  KR, Dietzschold  B. Effective preexposure and postexposure prophylaxis of rabies with a highly attenuated recombinant rabies virus. Proc Natl Acad Sci U S A. 2009;106:113005. DOIPubMed
  38. Lafon  M. Rabies virus receptors. J Neurovirol. 2005;11:827. DOIPubMed
  39. Tuffereau  C, Schmidt  K, Langevin  C, Lafay  F, Dechant  G, Koltzenburg  M. The rabies virus glycoprotein receptor p75NTR is not essential for rabies virus infection. J Virol. 2007;81:1362230. DOIPubMed
  40. Kuzmin  IV, Shi  M, Orciari  LA, Yager  PA, Velasco-Villa  A, Kuzmina  NA, Molecular inferences suggest multiple host shifts of rabies viruses from bats to mesocarnivores in Arizona during 2001–2009. PLoS Pathog. 2012;8:e1002786 . DOIPubMed

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1Joint senior authors who contributed equally to this article.

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