Volume 20, Number 6—June 2014
Dispatch
Fatal Monkeypox in Wild-Living Sooty Mangabey, Côte d’Ivoire, 2012
Aleksandar Radonić, Sonja Metzger, Piotr Wojtek Dabrowski, Emmanuel Couacy-Hymann, Livia Schuenadel, Andreas Kurth, Kerstin Mätz-Rensing, Christophe Boesch, Fabian H. Leendertz, and Andreas Nitsche
Table
Results of quantitative PCR from tissues of a wild-living sooty mangabey (Cercocebus atys), Taï National Park, Côte d’Ivoire, March 2012*
| Tissue | Ct OPV rpo18 | Ct c-myc | ΔCt, rpo18–c-myc |
|---|---|---|---|
| Spleen | 32.0 | 22.1 | –9.9 |
| Lung | 34.3 | 27.8 | –6.5 |
| Kidney | 28.2 | 18.9 | –9.3† |
| Skin | 16.9 | 26.9 | 9.9 |
| Liver | 30.3 | 20.0 | –10.3† |
| Heart | 32.2 | 25.0 | –7.2 |
| Intestine | 32.2 | 31.1 | 0.1 |
| Muscle | ND | ND | ND |
| Thymus | 15.8 | 19.7 | 3.9† |
| Throat swab | 21.5 | 28.4 | 6.9† |
| Lymph node | 24.1 | 20.2 | –3.9† |
*Virus DNA (rpo18) was quantified in relation to cellular c-myc DNA in 1 μL DNA; higher values indicate higher virus loads in a respective tissue. Ct, cycle threshold; OPV, orthopoxvirus; ND, OPV DNA not detectable.
†5 μL used in PCR.
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