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Volume 20, Number 6—June 2014
Dispatch

MERS Coronaviruses in Dromedary Camels, Egypt

Daniel K.W. Chu1, Leo L.M. Poon1, Mokhtar M. Gomaa, Mahmoud M. Shehata, Ranawaka A.P.M. Perera, Dina Abu Zeid, Amira S. El Rifay, Lewis Y. Siu, Yi Guan, Richard J. Webby, Mohamed A. Ali, Malik PeirisComments to Author , and Ghazi KayaliComments to Author 
Author affiliations: The University of Hong Kong, Hong Kong, China (D.K.W. Chu, L.L.M. Poon, R.A.P.M. Perera, Y. Guan, M. Peiris); National Research Centre, Giza, Egypt (M.M. Gomaa, M.M. Shehata, D.A. Zeid, A.S. El Rifay, M.A. Ali); HKU-Pasteur Research Pole, Hong Kong (L.Y. Siu); St. Jude Children’s Research Hospital, Memphis, Tennessee, USA (R.J. Webby, G. Kayali)

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Figure 2

Phylogenetic analyses of Middle East respiratory syndrome coronavirus (MERS-CoV) from dromedary camels. Genomic (A), spike (B), and nucleocapsid (C) sequences of the dromedary camel MERS-CoV NRCE-HKU205 (GenBank accession no. KJ477102) were aligned with the corresponding human MERS-CoV (N = 25) sequences retrieved from GenBank (accession nos. as in Figure 1 legend). Phylogenetic trees were constructed by using MEGA5 (14) with neighbor-joining method. Numbers at nodes indicate bootstrap values de

Figure 2. Phylogenetic analyses of Middle East respiratory syndrome coronavirus (MERS-CoV) from dromedary camelsGenomic (A), spike (B), and nucleocapsid (C) sequences of the dromedary camel MERS-CoV NRCE-HKU205 (GenBank accession noKJ477102) were aligned with the corresponding human MERS-CoV (N = 25) sequences retrieved from GenBank (accession nosas in Figure 1 legend)Phylogenetic trees were constructed by using MEGA5 (14) with neighbor-joining methodNumbers at nodes indicate bootstrap values determined by 500 replicatesOnly bootstrap values >70 are denotedBold type indicates MERS-CoV identified in the current studyScale bars indicate the estimated genetic distance of these viruses.

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References
  1. World Health Organization. Middle East respiratory syndrome coronavirus (MERS-CoV) summary and literature update—as of 20 January 2014 [cited 2014 Feb 20]. http://www.who.int/csr/disease/coronavirus_infections/MERS_CoV_Update_20_Jan_2014.pdf
  2. Assiri  A, McGeer  A, Perl  TM, Price  CS, Al-Rabeeah  AA, Cummings  DAT, Hospital outbreak of Middle East respiratory syndrome coronavirus. N Engl J Med. 2013;369:407–16 and. DOIPubMedGoogle Scholar
  3. Cotten  M, Watson  SJ, Kellam  P, Al-Rabeeah  AA, Makhdoom  HQ, Assiri  A, Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet. 2013;382:1993–2002 and. DOIPubMedGoogle Scholar
  4. Annan  A, Baldwin  HJ, Corman  VM, Klose  SM, Owusu  M, Nkrumah  EE, Human betacoronavirus 2c EMC/2012–related viruses in bats, Ghana and Europe. Emerg Infect Dis. 2013;19:456–9 and. DOIPubMedGoogle Scholar
  5. Ithete  NL, Stoffberg  S, Corman  VM, Cottontail  VM, Richards  LR, Schoeman  MC, Close relative of human Middle East respiratory syndrome coronavirus in bat, South Africa. Emerg Infect Dis. 2013;19:1697–9 and. DOIPubMedGoogle Scholar
  6. Memish  ZA, Mishra  N, Olival  KJ, Fagbo  SF, Kapoor  V, Epstein  JH, Middle East respiratory syndrome coronavirus in bats, Saudi Arabia. Emerg Infect Dis. 2013;19:1819–23 and. DOIPubMedGoogle Scholar
  7. Reusken  CBEM, Haagmans  BL, Müller  MA, Gutierrez  C, Godeke  G-J, Meyer  B, Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study. Lancet Infect Dis. 2013;13:859–66 and. DOIPubMedGoogle Scholar
  8. Perera  RA, Wang  P, Gomaa  MR, El-Shesheny  R, Kandeil  A, Bagato  O, Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013. Euro Surveill. 2013;18:20574 . DOIPubMedGoogle Scholar
  9. Reusken  CB, Ababneh  M, Raj  VS, Meyer  B, Eljarah  A, Abutarbush  S, Middle East respiratory syndrome coronavirus (MERS-CoV) serology in major livestock species in an affected region in Jordan, June to September 2013. Euro Surveill. 2013;18:20662 . DOIPubMedGoogle Scholar
  10. Hemida  MG, Perera  RA, Wang  P, Alhammadi  MA, Siu  LY, Li  M, Middle East respiratory syndrome (MERS) coronavirus seroprevalence in domestic livestock in Saudi Arabia, 2010 to 2013. Euro Surveill. 2013;18:20659 . DOIPubMedGoogle Scholar
  11. Meyer  B, Müller  MA, Corman  VM, Reusken  CBEM, Ritz  D, Godeke  G-D, Antibodies against MERS coronavirus in dromedary camels, United Arab Emirates, 2003 and 2013. Emerg Infect Dis [Internet]. 2014 Apr [cited 2014 Feb 22]. DOIGoogle Scholar
  12. Haagmans  BL, Al Dhahiry  SH, Reusken  CB, Raj  VS, Galiano  M, Myers  R, Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Lancet Infect Dis. 2014;14:140–5 and. DOIPubMedGoogle Scholar
  13. Chu  DK, Leung  CY, Gilbert  M, Joyner  PH, Ng  EM, Tse  TM, Avian coronavirus in wild aquatic birds. J Virol. 2011;85:12815–20 and. DOIPubMedGoogle Scholar
  14. Tamura  K, Peterson  D, Peterson  N, Stecher  G, Nei  M, Kumar  S. MEGA5: Molecular Evolutionary Genetics Analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 2011;28:2731–9 and. DOIPubMedGoogle Scholar

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1These authors contributed equally to this article.

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