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Volume 20, Number 7—July 2014
Letter

Stability of Middle East Respiratory Syndrome Coronavirus in Milk

Neeltje van Doremalen, Trenton Bushmaker, William B. Karesh, and Vincent J. MunsterComments to Author 
Author affiliations: National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA (N. van Doremalen, T. Bushmaker, V.J. Munster); EcoHealth Alliance, New York, New York, USA (W.B. Karesh)

Main Article

Figure

Viability of MERS-CoV in unpasteurized milk. MERS-CoV strain Jordan-N3/2012 was diluted in milk or DMEM to a final TCID50 of 105.5/mL and stored at either 4°C (A) or 22°C (B). MERS-CoV titer was determined at 0, 8, 24, 48, and 72 hours post dilution in quintuplicate. Red indicates dromedary camel milk; blue indicates goat milk; green indicates cow milk; black indicates DMEM. C) Milk containing MERS-CoV was pasteurized by heating 1-mL aliquots of diluted virus at 63°C for 30 min in triplicate. Re

Figure. Viability of MERS-CoV in unpasteurized milkMERS-CoV strain Jordan-N3/2012 was diluted in milk or DMEM to a final TCID50 of 105.5/mL and stored at either 4°C (A) or 22°C (B)MERS-CoV titer was determined at 0, 8, 24, 48, and 72 hours post dilution in quintuplicateRed indicates dromedary camel milk; blue indicates goat milk; green indicates cow milk; black indicates DMEMC) Milk containing MERS-CoV was pasteurized by heating 1-mL aliquots of diluted virus at 63°C for 30 min in triplicateRed indicates unpasteurized; blue indicates pasteurizedInfectious virus titers were determined by endpoint titration on Vero E6 cells in triplicateDotted line depicts the detection limit of the assayMERS-CoV, Middle East respiratory syndrome coronavirus; TCID50, 50% tissue culture infective dose; DMEM, Dulbecco modified Eagle medium.

Main Article

Page created: June 18, 2014
Page updated: June 18, 2014
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