Volume 20, Number 8—August 2014
Novel Reassortant Influenza A(H5N8) Viruses in Domestic Ducks, Eastern China
||Virus replication in experimentally infected mice, virus titers in organs of mice (log10 EID50/mL)†
|IVPI||EID50||TCID50||Tissue||3 d||6 d||9 d|
|W24||3.0||108.5||108.4||Lung||2.5 ± 0.5||3.0 ± 0.5||5.0 ± 0.5|
|6D18||3.0||108.5||109.6||Lung||2.0 ± 0.5||2.5 ± 0.5||3.0 ± 0.5|
|NA||NA||NA||Liver||–||–||2.0 ± 0.5|
*Groups of 14 adult female BALB/c mice were intranasally inoculated with a 106 median egg infective dose (EID50) of each virus in a 0.05-mL volume of phosphate-buffered saline. Five mice per group were observed for survival within 14 d. The remaining 9 mice from each experimental group were exsanguinated on days 3, 6, and 9, and their lungs, brains, hearts and livers were collected for virus titration in embryonated chicken eggs. IVPI, intravenous pathogenicity index; TCID50, median tissue culture infective dose; NA, not applicable.
†Values are mean ± SD. –, virus was not detected.