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Volume 20, Number 9—September 2014

Mutations of Novel Influenza A(H10N8) Virus in Chicken Eggs and MDCK Cells

Jian Yang1, Ting Zhang1, Li Guo1, Yongfeng Hu1, Jinlin Li, Haoxiang Su, Yan Xiao, Xianwen Ren, Jie Dong, and Lilian Sun
Author affiliations: MOH Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China (J. Yang, T. Zhang, L. Guo, Y. Hu, J. Li, H. Su, Y. Xiao, X. Ren, J. Dong, L. Sun, Y. Xiao, L. Li, F. Yang, J. Wang, Q. Jin); Jiangxi Provincial Center for Disease Control and Prevention, Nanchang, China (H. Yuan)

Main Article


Mutations and heterogeneities of influenza A(H10N8) virus detected in a tracheal aspirate sample from a human, the first passage in embryonated chicken eggs, and the second and third passages in MDCK cells

Segment* Nucleotide position† Direct sequencing Embryonated chicken eggs P1 MDCK cells
Amino acid change‡
P2 P3
PB2 1,879 A>G A>G A A E627K§
PA 1,689 G G A>G A>G
PA 1,995 G G A>G A>G
PA 1,997 T T C>T C>T L666P
HA 688 A A G>A G>A R220G
NP 583 C C A>C A>C
NP 1,405 G G A≥G A≥G E469K
NA 739 C C C>T C>T A246V§
NA 877 G G A≥G A≥G R292K
NA 1,187 C C C≥T C≥T
NS1 641 G>A G>A A≥G A≥G E208K

*PB, polymerase basic; PA, polymerase acidic; HA, hemagglutinin; NP, nucleocapsid protein; NA, neuraminidase; NS, nonstructural protein; –,synonymous substitution without amino acid change.
†Numbered from the first nucleotide of the determined genomic sequence.
‡Site positions are numbered from the start codon (M); H3 and N2 numbering for HA and NA, respectively.
§Potential amino acid change caused by the minor nucleotide in the heterogeneous site.

Main Article

1These authors contributed equally to this article.

2Co-senior authors.

Page created: August 14, 2014
Page updated: August 14, 2014
Page reviewed: August 14, 2014
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