Volume 20, Number 9—September 2014
Dispatch
Mutations of Novel Influenza A(H10N8) Virus in Chicken Eggs and MDCK Cells
Table
Mutations and heterogeneities of influenza A(H10N8) virus detected in a tracheal aspirate sample from a human, the first passage in embryonated chicken eggs, and the second and third passages in MDCK cells
| Segment* | Nucleotide position†| Direct sequencing | Embryonated chicken eggs P1 | MDCK cells |
Amino acid change‡ | |
|---|---|---|---|---|---|---|
| P2 | P3 | |||||
| PB2 | 1,879 | A>G | A>G | A | A | E627K§ |
| PA | 1,689 | G | G | A>G | A>G | – |
| PA | 1,995 | G | G | A>G | A>G | – |
| PA | 1,997 | T | T | C>T | C>T | L666P |
| HA | 688 | A | A | G>A | G>A | R220G |
| NP | 583 | C | C | A>C | A>C | – |
| NP | 1,405 | G | G | A≥G | A≥G | E469K |
| NA | 739 | C | C | C>T | C>T | A246V§ |
| NA | 877 | G | G | A≥G | A≥G | R292K |
| NA | 1,187 | C | C | C≥T | C≥T | – |
| NS1 | 641 | G>A | G>A | A≥G | A≥G | E208K |
*PB, polymerase basic; PA, polymerase acidic; HA, hemagglutinin; NP, nucleocapsid protein; NA, neuraminidase; NS, nonstructural protein; –,synonymous substitution without amino acid change.
†Numbered from the first nucleotide of the determined genomic sequence.
‡Site positions are numbered from the start codon (M); H3 and N2 numbering for HA and NA, respectively.
§Potential amino acid change caused by the minor nucleotide in the heterogeneous site.
1These authors contributed equally to this article.
2Co-senior authors.
Page created: August 14, 2014
Page updated: August 14, 2014
Page reviewed: August 14, 2014
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