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Volume 21, Number 1—January 2015
Dispatch

Foot-and-Mouth Disease Virus Serotype SAT 3 in Long-Horned Ankole Calf, Uganda

Moses Tefula Dhikusooka, Kirsten Tjørnehøj, Chrisostom Ayebazibwe, Alice Namatovu, Simon Ruhweza, Hans Redlef Siegismund, Sabenzia Nabalayo Wekesa, Preben Normann, and Graham J. BelshamComments to Author 
Author affiliations: Ministry of Agriculture Animal Industry and Fisheries, Entebbe, Uganda (M.T. Dhikusooka, C. Ayebazibwe, A. Namatovu, S. Ruhweza); Technical University of Denmark, Kalvehave, Denmark (K. Tjørnehøj, P. Normann, G.J. Belsham); Makerere University, Kampala, Uganda (A. Namatovu, S.N. Wekesa); University of Copenhagen, Copenhagen, Denmark (H.R. Siegismund); Ministry of Livestock Development, Nairobi, Kenya (S.N. Wekesa)

Main Article

Table 1

Detection of anti-FMDV in serum and FMDV RNA in probang samples from long-horned Ankole calves, Uganda, 2013*

Sampling
day†
 Calf no. 34
 Calf no. 33
Anti-NSP, PI
 SPBE titers‡ FMDV RNA in probang, Ct
Anti-NSP, PI
 SPBE titers† FMDV RNA in
probang, Ct
O
 SAT 1
 SAT 2
 SAT 3
 O
 SAT 1
 SAT 2
 SAT 3
0 22 – – – – – 35 – – – – –
14 55 – 160 – 80 21§ 25 <10 10 10 10 –
30 72 – 80 – 40 – 63 20 160 10 10 –

*Ct, cycle threshold; ELISA, enzyme-linked immunosorbant assay; FMDV, foot-and-mouth disease virus; PI, percentage inhibition values in the NSP ELISA (values >50% are considered positive and are indicated in boldface); NSP, nonstructural protein; RT-qPCR, real-time reverse transcription PCR; SAT, Southern African Territories; SPBE, solid-phase blocking ELISA; –, negative sample.
†Day 0 was defined as the day the sentinel animals arrived at the farm in Nyakatonzi (Kasese District).
‡An in-house serotype-specific SPBE was used; samples positive at 1:10 dilution were titrated; values are reciprocals of highest positive dilution.
§FMDV RNA in probang samples was assayed by RT-qPCR, and virus isolation was attempted from probang samples with detectable RNA and some other selected samples. The isolation of SAT 3 FMDV from the RT-qPCR–positive probang sample from calf no. 34 was demonstrated by serotype-specific antigen ELISA and confirmed by sequencing.

Main Article

Page created: December 19, 2014
Page updated: December 19, 2014
Page reviewed: December 19, 2014
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