Volume 21, Number 1—January 2015
Foot-and-Mouth Disease Virus Serotype SAT 3 in Long-Horned Ankole Calf, Uganda
||Calf no. 34
||Calf no. 33
||SPBE titers‡||FMDV RNA in probang, Ct
||SPBE titers†||FMDV RNA in
*Ct, cycle threshold; ELISA, enzyme-linked immunosorbant assay; FMDV, foot-and-mouth disease virus; PI, percentage inhibition values in the NSP ELISA (values >50% are considered positive and are indicated in boldface); NSP, nonstructural protein; RT-qPCR, real-time reverse transcription PCR; SAT, Southern African Territories; SPBE, solid-phase blocking ELISA; –, negative sample.
†Day 0 was defined as the day the sentinel animals arrived at the farm in Nyakatonzi (Kasese District).
‡An in-house serotype-specific SPBE was used; samples positive at 1:10 dilution were titrated; values are reciprocals of highest positive dilution.
§FMDV RNA in probang samples was assayed by RT-qPCR, and virus isolation was attempted from probang samples with detectable RNA and some other selected samples. The isolation of SAT 3 FMDV from the RT-qPCR–positive probang sample from calf no. 34 was demonstrated by serotype-specific antigen ELISA and confirmed by sequencing.