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Volume 21, Number 10—October 2015
Research

Possible Role of Rickettsia felis in Acute Febrile Illness among Children in Gabon

Gaël Mourembou, Jean Bernard Lekana-Douki, Oleg Mediannikov, Sydney Maghendji Nzondo, Lady Charlene Kouna, Jean Claude Biteghe Bi Essone, Florence Fenollar, and Didier RaoultComments to Author 
Author affiliations: Ecole Doctorale Régionale d’Afrique Centrale, Franceville, Gabon (G. Mourembou); Aix Marseille Université, Marseille, France (G. Mourembou, O. Mediannikov, F. Fenollar, D. Raoult); Université des Sciences de la santé de Libreville, Libreville, Gabon (J.B. Lekana-Douki); Centre International de Recherche Médicale de Franceville (CIRMF), Franceville (J.B. Lekana-Douki, S. Maghendji Nzondo, L.C. Kouna, J.C. Biteghe Bi Essone)

Main Article

Table 1

Primers and probes used in Rickettsia spp.–specific and R. felis–specific quantitative qPCR screening of blood samples from children <15 years of age in Gabon*

Pathogens, target gene Primers, forward; reverse 5'→3' Probe, 5'→3'
Rickettsia spp
RKND03 (gltA)
 GTGAATGAAAGATTACACTATTTAT
GTATCTTAGCAATCATTCTAATAGC
 6FAM-CTATTATGCTTGCGGCTGTCGGTTC-TAMRA
R. felis
bioB (0527) ATGTTCGGGCTTCCGGTATG
CCGATTCAGCAGGTTCTTCAA 6FAM-GCTGCGGCGGTATTTTAGGAATGGG-TAMRA
orfB CCCTTTTCGTAACGCTTTGCT
GGGCTAAACCAGGGAAACCT 6FAM-TGTTCCGGTTTTAACGGCAGATACCCA-TAMRA
vapB1 TGTCTTTCATGAATTGATCAGCA
AGGCGAAAGCTTTGACGTG 6FAM-AAGGCTTGGTTTCTGCGGGC-TAMRA

*The origin of the quantitative PCR is described in (14).

Main Article

Page created: September 22, 2015
Page updated: September 22, 2015
Page reviewed: September 22, 2015
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