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Volume 21, Number 12—December 2015
Letter

Characteristics of Traveler with Middle East Respiratory Syndrome, China, 2015

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To the Editor: A traveler returning from the Middle East initiated an outbreak of Middle East respiratory syndrome (MERS) in South Korea in 2015, which resulted in 186 cases and 36 deaths (13). We report a case of MERS in a 43-year-old man from South Korea who acquired this disease during this outbreak (Technical Appendix Figure 1) (4).

The National Health and Family Planning Commission of China determined that collection of data for this patient was part of a public health investigation of an emerging outbreak. Therefore, informed consent was not required. This study was approved by the ethical committee of the First Affiliated Hospital of Guangzhou Medical University.

The patient had been receiving thiamazole for 7 years for hyperthyroidism. He had contact with the index case-patient during the outbreak in South Korea on May 16, 2015. On May 25, the patient traveled to Hong Kong and then to Huizhou, China. He was hospitalized in China on May 28 (day 7 of illness). At admission, he had a high fever (temperature 39.5°C) and a dry cough. Chest radiography on day 7 showed mild bilateral ground glass opacities in the lower lung (Technical Appendix Figure 1, panel B).

The patient was given oseltamivir (150 mg, 2×/day for 2 days) until identified as being infected with Middle East respiratory syndrome coronavirus (MERS-CoV) on day 8 by real-time reverse transcription PCR. He was given ribavirin (2.0 mg on day 8; 0.6 mg 3×/d on days 9–16; and 0.6 mg 2×/d on days 17–19) and 135 μg of peginterferon αa-2a by intravenous injection on day 8 (Technical Appendix Table 2). Thrombocytopenia and a decrease in the hemoglobin level developed, which might have been related to use of ribavirin (Technical Appendix Table 1).

Chest radiography on June 1 (day 11) showed increased bilateral consolidation of the patient’s lower lung (Technical Appendix Figure 1, panel C). He was given intravenous immunoglobulin, antimicrobial drugs, and thymosin α1. His body temperature returned to normal on day 14 (Technical Appendix Figure 2). Chest radiography on day 35 showed resolution of bilateral lung infiltrations (Technical Appendix Figure 1, panel D). He was discharged on day 36.

Viral RNA was detected in sputum and fecal specimens up to day 26 of illness. Virus load (5) in sputum specimens collected on days 11–15 were lower than in specimens obtained on days 16–18 (Technical Appendix Figure 3, panel A). Swab samples collected on days 13 and 15 from the patient’s palm, mobile telephone, blanket, and bed railings, and from his hospital room floor were negative for viral RNA.

Concentrations of proinflammatory cytokines and chemokines (interferon-α, interferon-inducible protein 10, monocyte chemoattractant protein-1, interleukin 6 [IL-6], IL-10, tumor necrosis factor-α, IL-8, macrophage inflammatory protein-α [MIP-1α], MIP-1β, and IL-1β) were determined for serial serum samples. Interferon-α, interferon-inducible protein 10, monokine induced by interferon-γ, IL-6, monocyte chemoattractant protein-1, and IL-8 were detected on day 11 of illness but levels decreased as the patient clinically improved (Technical Appendix Figure 3, panel B).

The peginterferon αA2 the patient was given on day 8 might have influenced his plasma interferon-α levels (6). However, a previous study also showed increased levels of interferon-α in a patient who survived MERS-CoV infection but not in a person who died of MERS (7). Although MERS-CoV evades induction of innate immune responses by cell types, the virus elicits interferon responses in plasmacytoid dendritic cells in vitro (8). Levels of tumor necrosis factor-α, MIP-1α, MIP-1β, IL-10, and IL-1β did not increase in any of these specimens.

Peripheral blood mononuclear cells (PBMCs) obtained on day 24 of illness showed a strong specific T-cell response against MERS-CoV spike protein but not against severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein (Technical Appendix Figure 3, panel C). PBMCs from persons who were infected with SARS-CoV in 2003, as well as healthy persons, showed low-level T-cell responses against MERS-CoV spike protein, although some persons with a history of SARS still had detectable responses to SARS-CoV spike protein. It was reported that T-cell responses to SARS-CoV were directed against spike and nucleocapsid proteins (9). We did not have sufficient PBMCs to test T-cell responses against nucleocapsid protein.

Results for MERS-CoV antibody were negative at day 11 of illness by MERS-CoV spike pseudotype assay (MERS-S ppNT), microneutralization, 50% plaque reduction neutralization test (PRNT50), and S1 ELISA (EUROIMMUN AG, Lübeck, Germany). The patient showed seroconversion by day 14. MERS-S ppNT and PRNT50 provided earlier evidence of seroconversion (day 15) and higher antibody titers than the microneutralization, (day 18) (Technical Appendix Figure 3, panel D). Potent T-cell responses were elicited to MERS-CoV spike protein. These responses did not show cross-reactivity with SARS-CoV spike protein.

The MERS-S ppNT, which does not require Biosafety Level 3 containment, had sensitivity equivalent with that of PRNT50, which requires containment. Thus, MERS-S ppNT is a sensitive and specific assay for detecting neutralizing antibody against MERS-CoV. The sensitivity and specificity of this assay have been well-documented with serum samples from dromedary camels and other animals (10).

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Acknowledgment

This study was supported by the Science Research Project of the Guangdong Province (2013B020224006); the Municipal Science and Technology Bureau Foundation of Guangzhou (2014Y2-00031); the National Key Project of Clinical Faculty and Facility Construction on Infectious Diseases (2013–2014); and the National Institute of Allergy and Infectious Diseases, National Institutes of Health (HHSN272201400006C).

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Wen Da Guan1, Chris Ka Pun Mok1, Zi Lin Chen1, Li Qiang Feng, Zheng Tu Li, Ji Cheng Huang, Chang Wen Ke, Xilong Deng, Yun Ling, Shi Guan Wu, Xue Feng Niu, Ranawaka A Perera, Yuan Da Xu, Jincun Zhao, Lin Qi Zhang, Yi Min Li, Rong Chang Chen, Ghazi Kayali, Ling ChenComments to Author , and Nan Shan Zhong

Author affiliations: State Key Laboratory of Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China (W.D. Guan, Z.T. Li, S.G. Wu, X.F. Niu, Y.D. Xu, J. Zhao, Y.M. Li, R.C. Chen, L. Chen, N.S. Zhong); The University of Hong Kong, Hong Kong, China (C.K.P. Mok, R.A. Perera, M. Peiris); Huizhou Municipal Central Hospital, Huizhou, China (Z.L. Chen, Y. Ling); Guanzhou Institute of Biomedicine and Health, Guangzhou (L.Q. Feng, L. Chen); Guangdong Inspection and Quarantine Technology Center, Guangzhou (J.C. Huang); Guangdong Center for Disease Control and Prevention, Guangzhou (C.W. Ke); Guangzhou Eighth People‘s Hospital, Guangzhou (X. Deng); Tsinghua University School of Medicine, Beijing, China (L.Q. Zhang)

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References

  1. Cowling  BJ, Park  M, Fang  VJ, Wu  P, Leung  GM, Wu  JT. Preliminary epidemiological assessment of MERS-CoV outbreak in South Korea, May to June 2015. Euro Surveill. 2015;20:21163 . DOIPubMed
  2. Park  HY, Lee  EJ, Ryu  YW, Kim  Y, Kim  H, Lee  H, Epidemiological investigation of MERS-CoV spread in a single hospital in South Korea, May to June 2015. Euro Surveill. 2015;20:21169 . DOIPubMed
  3. World Health Organization. Middle East respiratory syndrome coronaviruses (MERS-CoV) [cited 2015 Sep 5]. http://www.who.int/emergencies/mers-cov/en/
  4. Wu  J, Yi  L, Zou  L, Zhong  H, Liang  L, Song  T, Imported case of MERS-CoV infection identified in China, May 2015: detection and lesson learned. Euro Surveill. 2015;20:21158 . DOIPubMed
  5. Memish  ZA, Al-Tawfiq  JA, Makhdoom  HQ, Assiri  A, Alhakeem  RF, Albarrak  A, Respiratory tract samples, viral load, and genome fraction yield in patients with Middle East respiratory syndrome. J Infect Dis. 2014;210:15904 . DOIPubMed
  6. El Sabaawy  D, El-Haggar  S. El-Bahrawy, Waked I, El-Said H. A comparative study of variants of pegylated interferon alpha in treatment of chronic HCV patients. APMIS. 2015;123:4829. DOIPubMed
  7. Faure  E, Poissy  J, Goffard  A, Fournier  C, Kipnis  E, Titecat  M, Distinct immune response in two MERS-CoV-infected patients: can we go from bench to bedside? PLoS ONE. 2014;9:e88716. DOIPubMed
  8. Scheuplein  VA, Seifried  J, Malczyk  AH, Miller  L, Höcker  L, Vergara-Alert  J, High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. J Virol. 2015;89:385969. DOIPubMed
  9. Channappanavar  R, Zhao  J, Perlman  S. T cell-mediated immune response to respiratory coronaviruses. Immunol Res. 2014;59:11828. DOIPubMed
  10. Perera  RA, Wang  P, Gomaa  MR, El-Shesheny  R, Kandeil  A, Bagato  O, Seroepidemiology for MERS coronavirus using microneutralisation and pseudoparticle virus neutralisation assays reveal a high prevalence of antibody in dromedary camels in Egypt, June 2013. Euro Surveill. 2013;18:20574 . DOIPubMed

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Cite This Article

DOI: 10.3201/eid2112.151232

1These authors contributed equally to this article.

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Table of Contents – Volume 21, Number 12—December 2015

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Ling Chen, State Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou, China

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Page created: November 17, 2015
Page updated: November 17, 2015
Page reviewed: November 17, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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