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Volume 21, Number 3—March 2015
Dispatch

Red Deer as Maintenance Host for Bovine Tuberculosis, Alpine Region

Maria FinkComments to Author , Corina Schleicher, Monika Gonano, Wolfgang M. Prodinger, Maria Pacciarini, Walter Glawischnig, Marie-Pierre Ryser-Degiorgis, Chris Walzer, Gabrielle L. Stalder, Dorotea Lombardo, Hermann Schobesberger, Petra Winter, and Mathias Büttner
Author affiliations: Austrian Agency for Health and Food Safety, Moedling, Austria (M. Fink, M. Gonano); Austrian Agency for Health and Food Safety, Graz, Austria (C. Schleicher); Innsbruck Medical University, Innsbruck, Austria (W.M. Prodinger); Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna, Brescia, Italy (M. Pacciarini); Austrian Agency for Health and Food Safety, Innsbruck (W. Glawischnig); University of Bern, Bern, Switzerland (M.-P. Ryser-Degiorgis); University of Veterinary Medicine, Vienna, Austria (C. Walzer, G.L. Stalder, H. Schobesberger, P. Winter); Istituto Zooprofilattico Sperimentale delle Venezie, Bolzano, Italy (D. Lombardo); Bavarian Health and Food Safety Authority, Oberschleißheim, Germany (M. Büttner)

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Abstract

To estimate the prevalence of bovine tuberculosis in the Alpine region, we studied the epidemiology of Mycobacterium caprae in wildlife during the 2009–2012 hunting seasons. Free-ranging red deer (Cervus elaphus) were a maintenance host in a hot-spot area, mainly located in Austria.

Bovine tuberculosis has one of the broadest host ranges of any known zoonotic pathogens. In addition to cattle, bovine tuberculosis affects many wild animal populations in North America, Europe, Africa, Asia, and New Zealand. Under certain conditions, wildlife play a role as reservoir and source of infection for domestic animals. Mycobacterium caprae has been isolated from cattle, domestic goats, domestic pigs, red deer (Cervus elaphus), and wild boar (1). Evidence is increasing that M. caprae is emerging in free-ranging red deer and cattle in the Alps (2,3).

The Study

To estimate the prevalence of bovine tuberculosis (which is caused by M. bovis and M. caprae) in wildlife in the Alps, we investigated 1,655 hunted red deer of both sexes and different ages in Austria, Germany, Switzerland (including the Principality of Liechtenstein), and Italy. The deer were hunted specifically for the study by trained hunters. A sampling/hunting plan was calculated in advance that indicated the number of animals needed in each sampling region to calculate prevalence estimates; the number was based on the red deer density of a region. The numbers of animals killed and sampled during 3 consecutive hunting seasons (2009–10, 2010–11, and 2011–12) coordinated nearly perfectly with the sampling plan that had been developed for each sampling area (Table 1). After pathomorphologic examination of carcasses (from Germany, Austria, Italy, Swiss Tessin) or samples (from Swiss St. Gall, Swiss Grisons, and Liechtenstein), we conducted microbiological analysis from sample material. Sample material included both medial retropharyngeal lymph nodes and tracheobronchial, mediastinal, and mesenteric lymph nodes and any other tissue with macroscopically visible lesions (Table 2). For bacteriologic cultivation the sample material was homogenized by using the IKA Ultra Turaxx Tube Drive System (Staufen, Germany), decontaminated with 1% N-acetyl-L-cystein solution and neutralized in phosphate buffer (pH 6.8) as recommended by the World Organisation for Animal Health (4). After sedimentation, inoculation was performed on 2 growth media: Stonebrink including PACT (polymyxin B, amphotericin B, carbenicillin, and trimethoprim) and Lowenstein-Jensen with glycerin and PACT (Heipha Diagnostika, Eppelheim, Germany). After 12 weeks’ incubation, a total of 82 bacterial cultures from 59 hunted red deer from Austria, Germany, and Italy were isolated (Tables 1, 2). All isolates were identified as M. caprae whether by reversed line blotting (Geno Type MTBC, HAIN Lifescience, Nehren, Germany) or by restriction fragment length polymorphism PCR of the gyrB gene, as previously described (5).

Red deer with macroscopically visible lesions (purulent abscesses varying remarkably in size) were all M. caprae positive from >1 lymph nodes/tissue, and 4 animals without visible lesions were M. caprae positive (Table 2). Microscopic examination showed that lesions had thin-walled fibrous capsules, occasionally with neutrophil granulocytes and calcifications in the necrosis zone, with an increased occurrence of neutrophil granulocytes and calcifications around the necrosis zone, and with a high occurrence of epithelioid and giant cells at the inside capsule wall. The large majority of these lesions were in the lymph nodes, particularly the medial retropharyngeal lymph nodes. Lesions in the lungs, observed in 3 animals, were consistently found in combination with lesions in the tracheobronchial and mediastinal lymph nodes; lesions in the tonsils were found with lesions in the retropharyngeal lymph nodes (Table 2). The medial retropharyngeal lymph nodes and the mesenteric lymph nodes were the primary sites of infection; most of the deer had lesions in the medial retropharyngeal lymph nodes and in the mesenteric lymph nodes, either exclusively or in combination with other sites (Table 2). Furthermore, the 4 animals without visible lesions were M. caprae positive from the medial retropharyngeal lymph nodes, suggesting a very early stage of infection.

M. bovis infections of cattle or deer tonsils led to bacterial colonization in the medial retropharyngeal lymph nodes with subsequent lymphatic spread to pulmonary lymph nodes. Ingestion of the M. bovis bacteria led to lymphatic spread from the primary infection, the gut, to mediastinal or tracheobronchial lymph nodes, which explained the lesions in the mesenteric lymph nodes (6). M. bovis causing tuberculosis is proposed to be a lymphatic disease (6); we propose the same for M. caprae.

Figure 1

Thumbnail of Study area in the Alpine region showing the 22 sampling areas and the estimated prevalences of Mycobacteria caprae. Prevalence ranges are classified into 6 intervals, wherein the upper bounds are not included in the interval. Austria: Vorarlberg (V1–V5) and Tyrolean Lech valley: Lechtal I (L1), Lechtal Mitte (LM), Lechtal II (L2), Schwarzwasser (SW),and Tannheimertal (TH). Germany: Bavaria (Region 1 and Region 2). Switzerland: St. Gall, Grisons, Tessin, and Liechtenstein. Italy: Var

Figure 1. Study area in the Alpine region showing the 22 sampling regions and the estimated prevalences of Mycobacteria caprae. Prevalence ranges are classified into 6 intervals, wherein the upper bounds are not...

Figure 2

Thumbnail of Statistically evident spatial cluster of Mycobacterium caprae–positive red deer in the Alpine region, 2009-10, 2010-2011, and 2011-12 hunting seasons. Area in red circle contained significantly more M. caprae–positive red deer than the remaining study area (p<0.001). Inset shows location of Austria and Germany within Europe (shading).

Figure 2. Statistically evident spatial cluster of Mycobacterium caprae–positive red deer in the Alpine region, 2009-10, 2010-2011, and 2011-12 hunting seasons. Area in red circle contained significantly more M. caprae–positive red deer than...

We calculated regional prevalence estimates with 95% CIs (Table 1; Figure 1). Prevalence estimates differed greatly among the sampling areas in Austria, Switzerland, Germany, and Italy. Moreover, a spatial cluster of positive samples (hot spot) around the Austrian sampling area Lechtal I was identified using point pattern analysis. The cluster comprised all positive animals from Lechtal I and the contiguous sampling areas of Lechtal Mitte, Regions 2 and 3 of Vorarlberg, and Region 1 of Bavaria (Germany) (p<0.001) (710). Consequently, this hot spot included 55 of the 59 positive samples detected in this study (Table 1; Figure 2).

Genotyping was performed from M. caprae isolates by spoligotyping (11) and by mycobacterial interspersed repetitive unit typing and variable number tandem repeat genotyping for 24 loci (12). All isolates in the hot-spot area and the 2 other isolates from Austria were of the Lechtal genotype. The single isolate in Italy, located >200 km from the hot spot, was also of the Lechtal genotype, and an association might exist between this case and cattle previously imported from Austria to Italy (13). By contrast, 2 isolates from Germany found in the Karwendel Mountains were of the Karwendel genotype. All other deer were negative for M. caprae and for M. bovis. After the transnational project, in the 2012–13 hunting season, the prevalence in Bavaria 1 (Germany) was 5.3%, indicating an expansion of or shift in the hot spot.

Conclusions

Our data indicate a localized bovine tuberculosis problem in wildlife in the Alps related to wildlife management strategies. Supplementary feeding is common in the hot-spot area to prevent migration and to keep red deer populations high (3). In the hot-spot area, the overall density is 5.6 animals/km2, but because of aggregation, which is considered the main cause of bovine tuberculosis maintenance in wildlife, winter habitats of red deer around feeding sites have up to 46.2 animals/km2 (Statistik Austria, http://www.statistik.at) (14). Low (0.5–2.5 and 2–4/km2) or medium densities (9.7 deer/km2) with prevalence estimates of 0% were found in Switzerland (Saint Gall, Liechtenstein, and Grisons, respectively) (14). Brescia, the only place in Italy where M. caprae was found in red deer, is considered a high-density region (16 animals/km2), but management strategies promoting aggregation are largely absent in Italy and Switzerland (13). Furthermore, red deer in the hot-spot area are a reservoir for bovine tuberculosis and are the source of M. caprae infections for domestic cattle grazing on Alpine pastures during the summer (3). Molecular-epidemiologic studies indicate that M. caprae isolates from red deer and cattle are of the same genotype (2). Our finding that retropharyngeal lymph nodes and mesenteric lymph nodes were often the primary site of infection suggests oral rather than aerosol transmission. Oral transmission does not require direct contact, either among red deer or between red deer and cattle.

Dr. Fink is a molecular biologist at the Austrian Agency for Health and Food Safety. Her primary research interests include epidemiology, emerging infectious disease, biosafety, and biosecurity.

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Acknowledgments

We thank Michael Schwarz for providing the maps, Sabrina Scheriau for processing statistical data, Barbara Pohl for providing technical assistance, and Alessandra Gaffuri and Mario Chiari for providing logistical support of the project.

This work was funded by the Emerging and Major Infectious Diseases in Livestock Animals ERA-Net (European Research Area–Net) Project “Tuberculosis in Alpine Wildlife,” together with the national founders of this project: the Austrian Ministry of Health, German Ministry of Education and Research, the Swiss Federal Veterinary Office, and the Italian Ministry of Health.

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References

  1. Aranaz  A, Liébana  E, Gómez-Mampaso  E, Galán  JC, Cousins  D, Ortega  A, Mycobacterium tuberculosis subsp. caprae subsp. nov.: a taxonomic study of a new member of the Mycobacterium tuberculosis complex isolated from goats in Spain. Int J Syst Bacteriol. 1999;49:126373 . DOIPubMed
  2. Prodinger  WM, Eigentler  A, Allerberger  F, Schonbauer  M, Glawischnig  W. Infection of red deer, cattle, and humans with Mycobacterium bovis subsp. caprae in western Austria. J Clin Microbiol. 2002;40:22702. DOIPubMed
  3. Schoepf  K, Prodinger  WM, Glawischnig  W, Hofer  E, Revilla-Fernández  S, Hofrichter  J, A two-years’ survey on the prevalence of tuberculosis caused by Mycobacterium caprae in red deer (Cervus elaphus) in the Tyrol, Austria. ISRN Vet Sci. 2012;2012:245138. PMID: 23762580
  4. World Organisation for Animal Health (OIE). OIE terrestrial manual 2009. Chapter 2.4.7. Bovine tuberculosis. 2009 [cited 2014 May 30]. http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.04.07_BOVINE_TB.pdf
  5. Niemann  S, Harmsen  D, Rüsch-Gerdes  S, Richter  E. Differentiation of clinical Mycobacterium tuberculosis complex isolates by gyrB DNA sequence polymorphism analysis. J Clin Microbiol. 2000;38:32314 .PubMed
  6. Behr  MA, Water  WR. Is tuberculosis a lymphatic disease with a pulmonary portal? Lancet Infect Dis. 2014;14:2505 . DOIPubMed
  7. Diggle  P. Statistical analysis of spatial point patterns. 2nd ed. London: Edward Arnold; 2003.
  8. Kelsall  JE, Diggle  P. Kernel estimation of relative risk. Bernoulli. 1995;1:316. DOI
  9. Kulldorf  M. A spatial scan statistic. Comm Stat Theory Methods. 1997;26:148796. DOI
  10. SaTScan v8.0: Software for the spatial and spacetime scan statistics. 2009 [cited 2014 May 30]. http://www.satscan.org
  11. Kamerbeek  J, Schouls  L, Kolk  A, Van Agterveld  M, Van Soolingen  D, Kuijper  S, Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. J Clin Microbiol. 1997;35:90714 .PubMed
  12. Supply  P, Allix  C, Lesjean  S, Cardoso-Oelemann  M, Rüsch-Gerdes  S, Willery  E, Proposal for standardization of optimized mycobacterial interspersed repetitive unit‐variable‐number tandem repeat typing of Mycobacterium tuberculosis. J Clin Microbiol. 2006;44:4498510 . DOIPubMed
  13. Chiari  M, Zanoni  M, Alborali  LG, Zanardi  G, Avisani  D, Tagliabue  S, Isolation of Mycobacterium caprae (Lechtal genotype) from red deer (Cervus elaphus) in Italy. J Wildl Dis. 2014;50:3303. DOIPubMed
  14. Schöning  JM, Cerny  N, Prohaska  S, Wittenbrink  MM, Smith  NH, Bloemberger  G, Surveillance of bovine tuberculosis and risk estimation of a future reservoir formation in wildlife in Switzerland and Liechtenstein. PLoS ONE. 2013;8:e54253. DOIPubMed

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Figures
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Cite This Article

DOI: 10.3201/eid2103.141119

Table of Contents – Volume 21, Number 3—March 2015

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Maria Fink, Austrian Agency for Health and Food Safety, Institute for Veterinary Disease Control, Robert Koch Gasse 17, A-2340 Moedling, Austria

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Page created: February 18, 2015
Page updated: February 18, 2015
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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