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Volume 21, Number 4—April 2015
Dispatch

High Seroprevalence of Antibodies against Spotted Fever and Scrub Typhus Bacteria in Patients with Febrile Illness, Kenya

Jacqueline W. Thiga, Beth K. Mutai, Wurapa K. Eyako, Zipporah Ng’ang’a, Ju Jiang, Allen L. Richards, and John N. WaitumbiComments to Author 
Author affiliations: Walter Reed Project/Kenya Medical Research Institute, Kisumu, Kenya (J.W. Thiga, B.K. Mutai, W.K. Eyako, J.N. Waitumbi); Jomo Kenyatta University of Agriculture and Technology, Itromid, Nairobi, Kenya (J.W. Thiga, Z. Ng’ang’a); Naval Medical Research Center, Silver Spring, Maryland, USA (J. Jiang, A.L. Richards)

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Figure 2

Western blot analysis using the Orientia spp.–specific antigen (Otr47b). Twenty scrub typhus reactive serum samples at a titer ≥1:6,000 were used. Negative controls were serum samples that were reactive to spotted fever and typhus group antigens. The scrub typhus reactive serum samples recognized the Otr47b antigen (lanes 2 and 3), but the spotted fever group and typhus group reactive serum samples did not (data not shown). Lane 1 was probed with a positive control serum sample from an earlier s

Figure 2. Western blot analysis using the Orientia spp.–specific antigen (Otr47b). Twenty scrub typhus reactive serum samples at a titer ≥1:6,000 were used. Negative controls were serum samples that were reactive to spotted fever and typhus group antigens. The scrub typhus reactive serum samples recognized the Otr47b antigen (lanes 2 and 3), but the spotted fever group and typhus group reactive serum samples did not (data not shown). Lane 1 was probed with a positive control serum sample from an earlier scrub typhus outbreak study (5). M, molecular mass standard, kDa.

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