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Volume 21, Number 4—April 2015
Synopsis

Hantavirus Pulmonary Syndrome , Southern Chile, 1995–2012

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Raúl Riquelme, María Luisa Rioseco, Lorena Bastidas, Daniela Trincado, Mauricio Riquelme, Hugo Loyola, and Francisca Valdivieso
Author affiliations: Universidad San Sebastian, Hospital de Puerto Montt, Puerto Montt,Chile (R. Riquelme, M.L. Rioseco, M Riquelme); Hospital de Puerto Montt, Puerto Montt (L. Bastidas, D. Trincado); Clínica Alemana Universidad del Desarrollo, Santiago, Chile (H. Loyola, F. Valdivieso)

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Abstract

Hantavirus is endemic to the Region de Los Lagos in southern Chile; its incidence is 8.5 times higher in the communes of the Andean area than in the rest of the region. We analyzed the epidemiologic aspects of the 103 cases diagnosed by serology and the clinical aspects of 80 hospitalized patients during 1995–2012. Cases in this region clearly predominated during winter, whereas in the rest of the country, they occur mostly during summer. Mild, moderate, and severe disease was observed, and the case-fatality rate was 32%. Shock caused death in 75% of those cases; high respiratory frequency and elevated creatinine plasma level were independent factors associated with death. Early clinical suspicion, especially in rural areas, should prompt urgent transfer to a hospital with an intensive care unit and might help decrease the high case-fatality rate.

Since the first cases described in United States in 1993, hantavirus pulmonary syndrome (HPS) has been reported in the United States, Argentina, Bolivia, Brazil, Chile, Ecuador, Paraguay, Panama, Uruguay, and Venezuela (1). Several types of New World hantaviruses (family Bunyaviridae) have been recognized. Their distribution is determined by the density of rodent populations serving as specific reservoirs of each virus type.

In Chile, Andes virus is the only identified hantavirus (2). It was first reported in 1995 during an outbreak in Argentina and is carried by the murid rodent Oligoryzomyslongicaudatus (i.e., long-tailed mouse or “colilargo”) in southern Argentina and central and southern Chile (3).

In Chile, where HPS is subject to immediate mandatory reporting to health authorities, a total of 786 cases occurred during 1995–2012. Regional and seasonal incidences varied from 0.17 to 0.53 cases per 100,000 inhabitants (4). Despite such low incidence, HPS is of public health concern because of its severity and its high case-fatality rate (CFR) (20%–60%).

We examined the clinical and epidemiologic features of HPS during 17 years in the provinces of Llanquihue and Palena, which had the highest incidences of this disease in Chile. This geographic area is served by the Health Service of Reloncaví (HSR) in Puerto Montt city, which has its 420-bed reference center at the Hospital of Puerto Montt in Puerto Montt.

Material and Methods

Study Site and Population

Figure 1

Thumbnail of The 13 communes in the provinces of Llanquihue and Palena, southern Chile. (Two communes share the name of the province to which they belong.) Asterisk indicates Andean communes. Inset: South America, with study area in box.

Figure 1. The 13 communes in the provinces of Llanquihue and Palena, southern Chile. (Two communes share the name of the province to which they belong.) Asterisk indicates Andean communes. Inset: South America,...

The provinces of Llanquihue and Palena are located in southern Chile, on the western edge of South America. Together they comprise 30,178 km2 and 340,464 inhabitants. These 2 provinces are subdivided into 13 communes (Figure 1).

Our study comprised all HPS cases reported to HSR during 1995–2012. All were confirmed by serologic tests performed at the National Reference Centers at the Public Health Institute (Santiago) or Universidad Austral (Valdivia). These tests are ELISAs for IgM and IgG that use hantavirus Sin Nombre antigen provided by the US Centers for Disease Control and Prevention (Atlanta, GA, USA).

Data Collection

We obtained data from 3 sources. First, we used epidemiologic records from all cases reported during 1995–2012. Data included patient age, sex, occupation, residence, site of probable infection, contact with other HPS patients, dates of hospitalization, and outcome.

Second, we reviewed clinical records of all patients admitted to Hospital of Puerto Monttwith confirmed HPS during the same period. Data recorded were age, sex, probable mechanism of infection, incubation period (only for patients for whom precise information about the time of rodent exposure and onset of symptoms was available), and medical history. On admission, presence of dyspnea, fever, asthenia, headache, myalgias, chills, cough, abdominal pain, and cyanosis and blood pressure, pulse, temperature, and respiratory frequency were recorded. During hospital stay, the following data were collected: presence of bleeding, alterations in renal and hepatic functions, admissions to intensive care unit (ICU), oxygen support, arterial oxygen tension/inspiratory oxygen fraction (PAFI) index, steroid administration, mechanical ventilation (MV) (specifying timing of connection), and circulatory shock. Shock was defined as systolic blood pressure <90 mm Hg that did not improve with fluid administration or that required the use of vasoactive drugs and abnormalities in tissue perfusion manifested by alteration of consciousness, oliguria, and lactate acidosis (5). One of the authors (R.R.) analyzed chest radiographs and classified the infiltrates as alveolar, interstitial, or mixed pattern and unilateral or bilateral; number of compromised quadrants and presence of pleural effusion were recorded. Results of laboratory tests performed on admission and during illness were also recorded. Each case was classified into 1 of 3 groups: grade I (mild disease) when patients had only prodromal symptoms without pulmonary involvement; grade II (moderate disease) when patients had interstitial pulmonary infiltrates or required supplemental oxygen but were hemodynamically stable; and grade III (severe disease) when patients required MV or had hemodynamic instability (6). Final outcome (death or survival) was also recorded.

Finally, we reviewed reports of epidemiologic inspections to homes, workplaces, and probable sites of infection (dwellings and their surroundings) at the time of case report to HSR. A survey administered to each patient or to close relatives asked about HPS risk during the 6 weeks before symptom onset. Visited places were classified as urban, rural, or semirural. We stratified the infection risk in visited dwellings according to a 5-parameter scale, each with 1 point assigned to absence of foundations, presence of holes, poor ventilation and lighting, presence of trash without adequate container inside the dwelling, and grainstorage, flour, and other food packaging Risk was considered high for scores 4–5, moderate for 2–3, low for 1, and absent for 0. We similarly classified dwelling surroundings according to presence of droppings, rodent pathways, rubbing stains, gnawing signs, rodent nests or holes, or observation of rodents themselves.

Statistical Analyses

We used the Student t test to compare parametric variables and χ2 and Fisher exact tests to compare discrete variables when necessary. A p value <0.05 was considered statistically significant. Incidence rate of HPS per commune was calculated from information provided by Chilean Census 2002 (7).

Results

During 1995–2012, a total of 103 confirmed HPS cases were reported to HSR. Mean age of patients was 35 ± 17 years (range 3–80 years); 71 (69%) were men. Overall CFR was 32% (33/103); CFR for the 80 HPS patients admitted toHospital of Puerto Montt was 30% (24/80).

Epidemiologic Characterization

We identified 52 rural locations as probable infection sites for 100 patients. For the remaining 3 patients, infection site could not be determined because of exposure to several risky sites.

Infection most likely was acquired through farming and forestry work for 44% of patients and was associated with recreational activities for 13%. For the remaining patients, infection-associated activity was not determined because of similar risk at home and at work.

HPS incidence per 100,000 inhabitants varied widely among communes. The highest rates occurred within Andean mountainous areas, mainly Palena and Cochamó communes (350 and 364 cases per 100,000 inhabitants, respectively). Incidence for the aforementioned communes was 8.5 times higher than that for the rest of the region (Table 1; Figure 1).

Figure 2

Thumbnail of Number of hantavirus pulmonary syndrome cases in provinces of Llanquihue and Palena, southern Chile, 1995–2012.

Figure 2. Number of hantavirus pulmonary syndrome cases in provinces of Llanquihue and Palena, southern Chile, 1995–2012.

Figure 3

Thumbnail of Seasonal incidence of hantavirus pulmonary syndrome, provinces of Llanquihue and Palena, Chile (n = 103), and entire country (n = 785), 1995–2012.

Figure 3. Seasonal incidence of hantavirus pulmonary syndrome, provinces of Llanquihue and Palena, Chile (n = 103), and entire country (n = 785), 1995–2012.

Yearly incidence varied widely during 1995–2012. Most cases occurred during 2005–2007 (Figure 2). In the region studied, incidence was highest during winter. By contrast, in the rest of the country, incidence was highest during summer and autumn (Figure 3).

For 23 patients, HPS occurred in related persons and made up a total of 8 clusters, 5 with 3 cases each and 4 with 2 cases each. All patients in each cluster shared both environmental risk factors and family relationship; included in the clusters were 6 cohabitating couples.

Epidemiologic reports on home or workplaces were available for 42 patients. Thirty-one percent of houses, 43% of housing environments, and 39% of working environments had high or moderate risk for rodent infestation.

Clinical Characteristics

The 80 HPS patients admitted to Hospital of Puerto Montt during 1995–2012 represented 78% of cases reported to HRS during that period. Seventy percent were men. Patients were 35.7 ± 16 years of age (range 4–73 years), and 90% were rural inhabitants. Their main occupational activities were farming or forestry (35%), housework (25%), student (15%), and fishery or marine harvesting (11%). Of these patients, 25 (31%) had a history of contact with rodents or rodent droppings. Infection was attributed to occupational exposure for 35 (44%) patients and traveling to a high incidence zone for 10 (12%).

Mean interval from appearance of symptoms to hospitalization was 5.7 ± 3 days (range 2–17 days). Incubation period, estimated from the analysis of 20 patients, was 10 ± 7 days (range 2–28 days).

For patients admitted during 1995–2004, HPS was considered 1of the admission diagnoses for only 7 (27%) of 26 case-patients. This presumptive diagnosis increased to 76% (37/49) during 2005–2012 (Tables 2, 3).

Duration of hospitalization for HPS patients was 5.2 ± 4.6 days (range 1–25 days). Sixty-three (79%) case-patients were admitted to the ICU; 72 (90%) required oxygen administration; and 40 (50%) were connected to MV for 4.2 ± 4.7 days (range 1–17 days).

Mean PAFI at admission was 216 ± 107 (range 40–508). Five (6%) patients had no pulmonary involvement. Shock occurred in 37 (46%) patients, all of whom received vasoactive drugs as prescribed. Hemorrhagic manifestations occurred in 31 (39%) patients: hematuria in 15 patients; cutaneous or puncture sites bleeding in 12 patients, hemoptysis in 5 patients, metrorrhagia in 4 patients; and epistaxis gingivorrhagia, rectorrhagia, and epidural hematoma after lumbar puncture in 1 patient each.

For 95% of patients, platelet counts were <100 × 103/μL(reference range 140–440103/μL) at a given time; for 34%, platelet counts were <35 × 103/μL. The mean platelet count was 50 ± 39 (range 8–238) × 103/μL. In 48% of hospitalized patients, creatinine increased >1.2 mg/dL (reference range 0.5–0.9 mg/dL); 4 (5%) of these patients required hemodialysis. Hepatic enzymes were elevated in 57 (71%) patients.

Thirty-one patients received steroids. Methylprednisolone was administered to 20 patients in accordance with a published protocol (8): 1 g intravenously per day for 3 days, followed by 16 mg orally per day for 3 days, 8 mg per day for 3 days, and 4 mg per day for 3 days. Eleven other patients were enrolled in a clinical trial and received methylprednisolone 1 g intravenously per day for 3 days (9). Nevertheless, we observed no difference in CFR between patients who did and did not receive methylprednisolone.

According to their clinical course, 5 (6%) patients were classified as having grade I HPS; 34 (42%) as having grade II HPS, and 41 (51%) as having grade III HPS. Twenty-four (30%) hospitalized patients died; for 21 (88%) of these, death was attributed directly to HPS. Shock was considered the cause of death for 18 (75%) patients, respiratory failure for 2 (8%), multiorgan failure for 2 (8%), and secondary sepsis for 2 (8%) (1 gram-negative sepsis and 1 Staphylococcus aureus sepsis).

Fifteen (63%) of 24 patients died during the first 24 hours after admission, and 22 (92%) died during the first 72 hours after admission. All deaths occurred among patients with grade III disease. Independent factors associated with death were respiratory frequency >30 breaths/minute and creatinine >1.3 mg/dL on admission (Table 4). CFR did not differ by sex, abnormal hepatic test results, or chest radiographic images progression >50% in 48 hours. Furthermore, we found no relation to CFR for patients with hematocrits >45% or >50%; platelet counts <100, <50 or <35 × 103/μL at admission; or PAFI <250, <200, <150, or <120 at admission.

Discussion

HPS is endemic in southern Chile, and human–rodent contact is considered the main mechanism for transmission. Several factors can explain the occurrence of human HPS in each of the 17 years of this study; these include a favorable habitat for rodent populations, which enables circulation of the virus between them (10), and high percentages of rural population (Llanquihue 27.5% and Palena 60%) for whom agriculture and forestry as the main occupational activities. Consequently, humans invade the rodents’ natural habitat, the temperate rain forest and its residues. Epidemiologic visits also found evidence of inadequate rural housing and peridomestic and workplace conditions that permitted rodent invasion; for 87% of patients, those places were considered the probable infection site. These findings reflect the usual conditions of life of the rural population and provide evidence that humans are exposed to hantavirus at home and in their workplaces. This contact is expected to increase if rodent population augments.

The incidence of cases varied in time. In Chile and southern Argentina, disease incidence has increased coincidence with the synchronic flowering and seeding of the shrub Chusqueaquila, a perennial bamboo that occurs in long inter annual cycles and provides abundant food for the granivorus rodent O. longicaudatus. Large outbreaks of rodents (known as “ratadas”) are associated with this cyclic phenomenon (10, 11), with existing chronicles as old as the conquest and Spanish colonization of the country (12).

In Chile, reported cases peaked in 2001, but in our study, cases peaked in 2005–2007. A possible explanation for the increase in HPS cases during 2005–2007 (Figure 3), without evidence of “ratada,” is that other native trees (olivillo, Aextoxiconpunctatum; avellano, Gevuinaavellana; and others), shrubs (“murtilla”), and herbs also have cycles of seed production not clearly known because they are not under surveillance and therefore not reported. These seasonal cycles could be associated with localized and minor differences in rodent population density (13). More research on the characteristics of the reservoir species and its habitat is needed to better understand the epidemiology of human disease

In the United States, HPS displays a strong seasonal distribution. Most cases occur in May, June, and July; the fewest occur in December, January, and February (14). In Chile, HPS incidence is also highest during the Southern Hemisphere summer (4) (Figure 3); the same has been described in Argentina (15). During previous years, brief periods of observation found this seasonal distribution in the study region (10). Seasonal distribution has been suggested to be a consequence of increasing human recreational and occupational activities in rural areas (10,16). However, we found that most cases occurred during autumn and winter, in coincidence with the increase in O. longicaudatus mice, resulting from increased seed availability. Otherwise, this rodent goes out into open spaces in summer to reproduce, favoring human contact on vacations and outdoor activities (17).

In our study, the increase in HPS cases during autumn and winter suggests a particular form of contagion. In the provinces studied here, humans live and work in the invading rodents’ habitat during times when rodents are more abundant.

Hantavirus seroprevalence in rodents varies by season and geography. Captures in our region during 1998–2001 showed seroprevalence rates of 7.2%–13.5%, which is higher than in the rest of the country (1.5%–3.2%). This prevalence could be even larger in O. longicaudatus mice captured in a patient’s home and peridomestic storage buildings (10).

The native landscape fragmentation caused by forestry and agriculture has favored the overgrowth and wider distribution of C. quila bamboo and the movement of O. longicaudatus mice between patches of vegetation, which increases the chance of human–rodent contact (18,19). This observation could explain the higher incidence of HPS in communes of our region exhibiting higher densities of C. quila bamboo and in the Andean region next to El Bolson, Argentina, where the first HPS cases in South America were reported, followed by the communes of Los Muermos and Fresia in the Cordillera de la Costa (Table 1; Figure 1).

Since identification of the first cases of HPS in HSR, clinicians have improved their initial diagnostic accuracy from 27% during 1995–2004 to 76% during 2005–2012. Accurate diagnosis is important because HPS is an unusual disease, even in a zone to which it is endemic, and early suspicion enables timely and effective management.

The disease characteristics we observed—fever, myalgias, thrombocytopenia, increased hematocrit, leukocytosis, and elevated creatinine, followed by different degrees of pulmonary involvement, usually with rapidly evolving acute respiratory distress—confirm what we described previously among 25 cases (6) and matches HPS descriptions from the United States (14,20). However, in the US cases, hemorrhagic manifestations were not described; in Chile, hemorrhagic manifestations have been repeatedly reported (6,8,21,22), even though in this study they were less frequent than in 2003 (39% vs. 64%, respectively). The 32% CFR is similar to that reported in the United States (35%) (14).

On the basis of an open study suggesting the benefit of high-dose steroids for HPS (8), we administered that dose to 31 of the patients reported here. We discontinued high-dose steroids after a controlled trial showed its failure to decrease the severity of HPS (9).

In this study of a large number of cases, we confirmed the variable characteristics of hantavirus disease, from only mild prodromal symptoms without cardiopulmonary involvement to the severe cardiopulmonary syndrome, as observed in a small number of cases studied previously in our region (6). Five patients in this study showed no evidence of pulmonary involvement, which is consistent with seroprevalence studies identifying hantavirus-seropositive persons without history of severe disease in up to 10% of some groups of Andean inhabitants in Chile (23). Reports on hantavirus seropositivity in persons without pulmonary involvement also have been presented in Argentina and the United States, although at lower frequencies (14,15). As long as pulmonary symptoms are required for reporting of hantavirus infection to the US Centers for Disease Control and Prevention, these milder hantavirus infections will continue to go uncounted (14). Causes for different clinical characteristics of hantavirus infection are under investigation and can be related to the virus, the human host, or both (24).

Severe hantavirus disease is characterized by a rapid installation and progression of severe respiratory failure and shock, which requires urgent ICU management, which is not always available in a timely manner. Death occurs in most cases within 1–2 days after hospital admission. Respiratory frequency >30 breaths/minute and creatinine levels >1.3 mg/dLon hospital admission were independent factors associated with death (Table 3).

In US studies, platelet count was significantly lower in patients who died than in those who survived, but we did not confirm this finding. Low platelet count and high hematocrit are good indicators for suspecting the diagnosis, but we did not correlate them with death. The presence of these 2 elements with compatible clinical and epidemiologic background should prompt rapid transfer of the patient to a hospital with ICU facilities. A rapid test to detect hantavirus IgM based on recombinant N-protein of Puumala virus (IgM POC PUUMALA, Reagena Ltd, Toivala, Finland) was evaluated for Andes virus diagnosis and showed >90% sensitivity and specificity It is available in Chile and is of help in some cases for decision making (25). Early clinical suspicion of hantavirus disease, especially in small rural areas, must indicate urgent transfer to a hospital with ICU and may help decrease the high CFR observed in patients with HPS.

Dr. RaúlRiquelme is associated professor of internal medicine and respiratory diseases in the School of Medicine, San SebastianUniversity, Hospital de Puerto Montt, Chile. His primary research interests are community-acquired pneumonia and hantavirus disease.

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Acknowledgment

We thank Roberto Murua for his valuables comments. We also thank Carolina Larrain for assistance in manuscript editing. We greatly appreciate the technical assistance of Marcela Amtmann.

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References

  1. Centers for Disease Control and Prevention. International HPS cases [cited 2013 Feb 1]. http://www.cdc.gov/hantavirus/surveillance/international.html
  2. Medina  RA. Torres-PerezF, GalenoH, NavarreteM, VialPA, PalmaRE, et al.Ecology, genetic diversity, and phylogeographic structure of andes virus in humans and rodents in Chile.J Virol. 2009;83:2446–59.PubMed
  3. Centers for Disease Control and Prevention. Outbreak of acute illness—southwestern United States, 1993. MMWR Morb Mortal Wkly Rep. 1993;42:4214 .PubMed
  4. Ministerio de Salud. Gobierno de Chile. El Vigía. 2012;13(27).
  5. Fang  GD, Fine  M, Orloff  J, Arisumi  D. YuVL, KpoorW, et al.New emerging etiologies for community-acquired pneumonia with implications for therapy.Medicine. 1990;69:307–16.
  6. Riquelme  R, Riquelme  M, Torres  A, Rioseco  ML, Vergara  JA, Scholz  L, Hantavirus pulmonary syndrome, southern Chile. Emerg Infect Dis. 2003;9:143843. DOIPubMed
  7. Instituto Nacional de Estadísticas. Censo 2002 [cited2013 Feb 1]. http://www.ine.cl/cd2002/
  8. Tapia  M, Mansilla  C, Vera  J. Síndrome pulmonar por hantavirus: experiencia clínica en diagnóstico y tratamiento. Hospital Coyhaique-Chile. Rev Chilena Infectol. 2000;17:25869. DOI
  9. Vial  PA, Valdivieso  F, Ferres  M, Riquelme  R, Rioseco  ML, Calvo  M, High-dose intravenous methylprednosolone for hantavirus cardiopulmonary syndrome in Chile: a double-blind, randomized controlled clinical trial. Clin Infect Dis. 2013;57:94351. DOIPubMed
  10. Murúa  R, Navarrete  M, Cádiz  R, Figueroa  R, Padula  P, Zaror  L, Sindrome pulmonar por hantavirus: situación de los roedores reservorios y la población humana en la Decima Región de Chile. Rev Med Chil. 2003;131:16976. DOIPubMed
  11. Murua  R, Gonzalez  L, Gonzalez  M, Jofre  C. Efectos del florecimiento del arbusto Chusquea quilaKunth (Poaceae) sobre la demografia de poblaciones de roedores de los bosques templados frios del sur chileno. Bol SocBiolConcepc. 1996;67:3742.
  12. Gonzales  Y, Gonzalez  M. Memoria y saber cotidiano. El florecimiento de la “quila” en el sur de Chile: de pericotes, ruinas y remedios.Revista Austral de Ciencias Sociales.2006;10:75–102.
  13. Glass  GE, Yates  TL, Fine  JB, Shields  TM, Kendall  JB, Hope  AG, Satellite imagery characterizes local animal reservoir populations on Sin Nombre virus in the southwestern United States. Proc Natl Acad Sci U S A. 2002;99:1681722. DOIPubMed
  14. MacNeilA. KsiasekT, RollinP. Hantavirus pulmonary syndrome, United States, 1993–2009. Emerg Infect Dis. 2011;17:1195201 and.PubMed
  15. Martinez  VP, Bellomo  CM, Cacace  ML, Suarez  P, Bogni  L, Padula  PJ. Hantavirus pulmonary síndrome in Argentina, 1995–2008. Emerg Infect Dis. 2010;16:185360. DOIPubMed
  16. Khan  AS, Khabbaz  RF, Armstrong  LR, Holman  RC, Bauer  SP, Graber  J, Hantavirus pulmonary syndrome: the first 100 US cases. J Infect Dis. 1996;173:1297303. DOIPubMed
  17. Murua  R. Ecología de los reservorios silvestres de hantavirus en Chile. Rev Chilena Infectol. 1998;15:7983.
  18. Langlois  JP, Fahrig  L, Merriam  G, Hartsob  H. Landscape structure influences continental distribution of hantavirus in deer mice. Landscape Ecol. 2001;16:25566. DOI
  19. Murua  R, Padula  P. Ecología y evolución de hantavirus en el Cono Sur de América. Arch Med Vet. 2004;36:120. DOI
  20. Knust  B, Rollin  PE. Twenty-year summary of surveillance for human hantavirus infections, United States. Emerg Infect Dis. 2013;19:19347. DOIPubMed
  21. Castillo  C, Naranjo  J, Ossa  G. Síndrome cardiopulmonar por hantavirus en 21 adultos en la IX región de Chile. Rev Chilena Infectol. 2000;17:2417. DOI
  22. Castillo  C, Naranjo  J, Ossa  G, Levi  H. Hantavirus pulmonarysyndromedue to Andes virus in Temuco, Chile. Chest. 2001;120:54854. DOIPubMed
  23. Baró  M, Vergara  J, Navarrete  M. Hantavirus en Chile: revisión y análisis de casos desde 1975. Rev Med Chil. 1999;61:26975.
  24. Krüger  DH, Schönrich  G, Klempa  B. Human pathogenic hantaviruses and prevention of infection. Hum Vaccin. 2011;7:68593. DOIPubMed
  25. Navarrete  M, Barrera  C, Zaror  L, Otth  C. Rapid immunochromatographic test for hantavirus andes contrasted with capture-IgM ELISA for detection of Andes-specific IgM antibodies. J Med Virol. 2007;79:414. DOIPubMed

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DOI: 10.3201/eid2104.141437

Table of Contents – Volume 21, Number 4—April 2015

Page created: March 17, 2015
Page updated: March 17, 2015
Page reviewed: March 17, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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