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Volume 21, Number 6—June 2015
Dispatch

Acute Middle East Respiratory Syndrome Coronavirus Infection in Livestock Dromedaries, Dubai, 2014

Sung Sup Park, Ulrich WerneryComments to Author , Victor M. Corman, Emily Y.M. Wong, Alan K.L. Tsang, Doreen Muth, Susanna K. P. Lau, Kamal Khazanehdari, Florian Zirkel, Mansoor Ali, Peter Nagy, Jutka Juhasz, Renate Wernery, Sunitha Joseph, Ginu Syriac, Shyna K. Elizabeth, Nissy Annie Georgy Patteril, Patrick C. Y. Woo, and Christian Drosten
Author affiliations: Central Veterinary Research Laboratory, Dubai, United Arab Emirates (U. Wernery, K. Khazanehdari, R. Wernery, S. Joseph, G. Syriac, S.K. Elizabeth, N.A. Georgy Patteril); University of Bonn Medical Centre, Bonn, Germany (V.M. Corman, F. Zirkel, C. Drosten); German Centre for Infection Research, Bonn (V.M. Corman, F. Zirkel, C. Drosten); University of Hong Kong, China (E.Y.M. Wong, A.K.L. Tsang, D. Muth, S.K.P. Lau, P.C.Y. Woo); Dubai Camel Hospital, Dubai (M. Ali); Emirates Industry for Camel Milk and Products, Dubai (P. Nagy, J. Juhasz)

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Table 1

Results of cross-sectional study of MERS-CoV antibodies and RNA and MERS-CoV isolation in dromedary camels at 3 sampling sites, Dubai, March–June, 2014*

Serum antibody detection by ELISA† RNA detection by RT-PCR‡ Virus isolation§
Adults, >4 298/310 (96.1) 0/250 (0) 0/12 (0)
Subadults, 2–4 328/340 (96.5) 10/344 (2.9) ¶ 1/14 (7.1)
Calves, <1 92/108 (85.2)¶ 24/68 (35.3)¶ 6/44 (13.6) ¶
Unknown 68/85 (80) 11/209 (5.3) 1/12 (8.3)
Total 786/843 (93.2) 45/871 (5.1) 8/82 (9.6)

*MERS-CoV, Middle East respiratory syndrome coronavirus; RT-PCR, reverse transcription PCR.
†ELISA used a recombinant MERS-CoV globular spike (S1) domain as described by Drosten et al. (11), modified by the reagent manufacturer (EUROIMMUN, Lübeck, Germany) for application with camel serum as validated by Corman et al. (12) and Memish et al (7). The anti-human conjugate was replaced by an anti-camel conjugate. The test was not selective for IgG.
‡RT-PCR targeting regions upstream of the envelope gene, as described by Corman et al. (13).
§Method as described by Drosten et al. (14).
¶Significantly different from grand mean (shown under Total), p<0.05 (χ2 test)

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References
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Page created: May 15, 2015
Page updated: May 15, 2015
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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