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Volume 21, Number 7—July 2015
Letter

Characterization of 3 Megabase-Sized Circular Replicons from Vibrio cholerae

Kazuhisa OkadaComments to Author , Wirongrong Natakuathung, Mathukorn Na-Ubol, Amonrattana Roobthaisong, Warawan Wongboot, Fumito Maruyama, Ichiro Nakagawa, Siriporn Chantaroj, and Shigeyuki Hamada
Author affiliations: Thailand–Japan Research Collaboration Center on Emerging and Re-emerging Infections, Nonthaburi, Thailand (K. Okada, W. Natakuathung, M. Na-Ubol, A. Roobthaisong, W. Wongboot, S. Hamada); Osaka University, Osaka, Japan (K. Okada, S. Hamada); Tokyo Medical and Dental University, Tokyo, Japan (F. Maruyama, I. Nakagawa); National Institute of Health, Nonthaburi (S. Chantaroj)

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Figure

Pulsed-field gel electrophoresis of intact genomic DNA of Vibrio cholerae isolates and circular representation of the genome of V. cholerae O1 El Tor TSY216, consisting of 3 chromosomes. The preparation of genomic DNA embedded in agarose gels and the protocol for pulsed-field gel electrophoresis have been described previously (5). Arrows indicate DNA bands that correspond to the chromosomes. Lanes: 1, N16961 reference strain carrying Ch1 (2.96 Mb) and Ch2 (1.07 Mb) (2); 2, TSY216; 3, TSY241; 4,

Figure. Pulsed-field gel electrophoresis of intact genomic DNA of Vibrio cholerae isolates and circular representation of the genome of V. cholerae O1 El Tor TSY216, consisting of 3 chromosomes. The preparation of genomic DNA embedded in agarose gels and the protocol for pulsed-field gel electrophoresis have been described previously (5). Arrows indicate DNA bands that correspond to the chromosomes. Lanes: 1, N16961 reference strain carrying Ch1 (2.96 Mb) and Ch2 (1.07 Mb) (2); 2, TSY216; 3, TSY241; 4, TSY421. The first and second outermost 2 circles of each schematic chromosome show the COGs, functional categories of the coding regions of TSY216, in the clockwise and anticlockwise directions, respectively. The third and fourth outermost circles of each schematic chromosome show the coding sequence-assigned (blue) and coding sequences-unassigned (red) functions of the products, respectively. The third and fourth circles show the GC content of the TSY216 sequence and the percent G+C deviation according to the strand, respectively. Ch1, chromosome 1; Ch2, chromosome 2; COGs, clusters of orthologous groups of proteins; Rep.3, novel replicon.

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References
  1. Kolstø  AB. Time for a fresh look at the bacterial chromosome. Trends Microbiol. 1999;7:223–6. DOIPubMedGoogle Scholar
  2. Heidelberg  JF, Eisen  JA, Nelson  WC, Clayton  RA, Gwinn  ML, Dodson  RJ, DNA sequence of both chromosomes of the cholera pathogen Vibrio cholerae. Nature. 2000;406:477–83. DOIPubMedGoogle Scholar
  3. Yamaichi  Y, Iida  T, Park  KS, Yamamoto  K, Honda  T. Physical and genetic map of the genome of Vibrio parahaemolyticus: presence of two chromosomes in Vibrio species. Mol Microbiol. 1999;31:1513–21. DOIPubMedGoogle Scholar
  4. Yamaichi  Y, Gerding  MA, Davis  BM, Waldor  MK. Regulatory cross-talk links Vibrio cholerae chromosome II replication and segregation. PLoS Genet. 2011;7:e1002189. DOIPubMedGoogle Scholar
  5. Okada  K, Iida  T, Kita-Tsukamoto  K, Honda  T. Vibrios commonly possess two chromosomes. J Bacteriol. 2005;187:752–7. DOIPubMedGoogle Scholar
  6. Okada  K, Roobthaisong  A, Nakagawa  I, Hamada  S, Chantaroj  S. Genotypic and PFGE/MLVA analyses of Vibrio cholerae O1: geographical spread and temporal changes during the 2007–2010 cholera outbreaks in Thailand. PLoS ONE. 2012;7:e30863. DOIPubMedGoogle Scholar
  7. Reimer  AR, Van Domselaar  G, Stroika  S, Walker  M, Kent  H, Tarr  C, Comparative genomics of Vibrio cholerae from Haiti, Asia, and Africa. Emerg Infect Dis. 2011;17:2113–21. DOIPubMedGoogle Scholar
  8. Mutreja  A, Kim  DW, Thomson  NR, Connor  TR, Lee  JH, Kariuki  S, Evidence for several waves of global transmission in the seventh cholera pandemic. Nature. 2011;477:462–5. DOIPubMedGoogle Scholar
  9. Doyle  M, Fookes  M, Ivens  A, Mangan  MW, Wain  J, Dorman  CJ. An H-NS-like stealth protein aids horizontal DNA transmission in bacteria. Science. 2007;315:251–2. DOIPubMedGoogle Scholar
  10. Chun  J, Grim  CJ, Hasan  NA, Lee  JH, Choi  SY, Haley  BJ, Comparative genomics reveals mechanism for short-term and long-term clonal transitions in pandemic Vibrio cholerae. Proc Natl Acad Sci U S A. 2009;106:15442–7. DOIPubMedGoogle Scholar

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