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Volume 21, Number 8—August 2015

Detection and Full-Length Genome Characterization of Novel Canine Vesiviruses

Vito MartellaComments to Author , Pierfrancesco Pinto, Eleonora Lorusso, Barbara Di Martino, Qiuhong Wang, Vittorio Larocca, Alessandra Cavalli, Michele Camero, Nicola Decaro, Krisztián Bányai, Linda J. Saif, and Canio Buonavoglia
Author affiliations: Università Aldo Moro di Bari, Valenzano, Italy (V. Martella, P. Pinto, E. Lorusso, V. Larocca, A. Cavalli, M. Camero, N. Decaro, C. Buonavoglia); Università degli Studi di Teramo, Teramo, Italy (B. Di Martino); The Ohio State University, Wooster, Ohio, USA (Q. Wang, L.J. Saif); Hungarian Academy of Sciences, Budapest, Hungary (K. Bányai)

Main Article


Primers used in study of the detection and full-length genome characterization of novel canine vesiviruses

Primer Position* Sequence Sense Use†
493F 5127–5150 GGT TTG CCA TCT GGC ATG CCG CTA + Detection
526R 5793–5816 AGC CAT VGC TCA RTT CTC AAA CAC Detection
p289 5271–5292 TGA CAA TGT AAT CAT CAC CAT A Detection
p290 4962–4984 GAT TAC TCC AAG TGG GAC TCC AC + Detection
501F 554–577 GTC TTG TGC TCT VTA CGA CAM ATG + Sequencing
VN3T20 3′- poly A GAG TGA CCG CGG CCG CT20. 3′ RACE
502F 1215–1234 ATG ATW ATT GAH AAC CAY GA + Sequencing
509F 2904–2923 TAC GAT ATG GCY TGG GCY CT + Sequencing
510F 3411–3432 GAT GAT GAG TAC GAT GAR TGG A + Sequencing
511F 3588–3607 GAA GAC GTC ACC RTA ATT GG + Sequencing
513F 3876–3898 GTT ACG TTC RAT GGY GAA TTG GC + Sequencing
515F 4314–4338 CAC GTG TCA CCA GCA CAC RTD GAT G + Sequencing
504R 1599–1618 ACC ACG CTY TCR TTS GAC CA Sequencing
528R 2887–2909 CTT GTC ATC TTA GTG TAC AAT GA Sequencing
506R 1680–1702 AG GTT GGT RAC NGC RTC AAT GTC Sequencing
508R 1974–1993 GT GTA GGC RYC GTG GTG GTC Sequencing
512R 3588–3608 GCC AAT TAY GGT GAC RTC WTC Sequencing
536F 714–732 TAC GAT CTT GCA ATC AAT G + Sequencing
GE72F 1915–1935 CCT ATG CCA TTG CGT CTA GAC + Sequencing
GE73R 2951–2970 CAG CCT TAA GTG CCT GCC AC Sequencing
SEQ100 5634–5653 TGT CGC CAA ATG TTG ATG AG + Sequencing
SEQ101 6343–6360 TTG CCA CAG GCA CTC AGC + Sequencing
SEQ102 6860–6877 GGA AAC ACG TGG TGG TCA + Sequencing
SEQ103 7487–7504 AAG TAG AAT GAT TGG TGA + Sequencing
SEQ104 8063–8082 GAG TTT GAC AAG ATG AAC AG + Sequencing

*Position is based on the sequence of strain Bari/212/07/ITA (GenBank accession no. JN204722).
†RACE, rapid amplification of cDNA ends.

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Page created: July 15, 2015
Page updated: July 15, 2015
Page reviewed: July 15, 2015
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