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Volume 21, Number 9—September 2015
Dispatch

Putative Lineage of Novel African Usutu Virus, Central Europe

Daniel Cadar, Stefan Bosch, Hanna Jöst, Jessica Börstler, Mutien-Marie Garigliany, Norbert Becker, and Jonas Schmidt-ChanasitComments to Author 
Author affiliations: Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany (D. Cadar, H. Jöst, J. Börstler, M.-M. Garigliany, J. Schmidt-Chanasit); Nature and Biodiversity Conservation Union, Stuttgart, Germany (S. Bosch); University of Liège, Liège, Belgium (M.-M. Garigliany); German Mosquito Control Association, Speyer, Germany (N. Becker); German Centre for Infection Research, Hamburg (H. Jöst, J. Schmidt-Chanasit)

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Figure 2

Phylogenetic relationship of the Usutu virus Usutu-BONN strain (from Germany, 2014) and other Usutu viruses, based on complete amino acid sequences of the polyprotein-encoding gene. The phylogenetic tree was constructed by using the maximum-likelihood method PhyML 3.0 (12) with 1,000 pseudoreplicates and, in parallel, Bayesian Markov chain Monte Carlo tree-sampling methods by using MrBayes 3.1.2 (13). The Akaike information criterion was chosen as the model selection framework, and the Johnes-Ta

Figure 2. Phylogenetic relationship of the Usutu virus Usutu-BONN strain (from Germany, 2014) and other Usutu viruses, based on complete amino acid sequences of the polyprotein-encoding gene. The phylogenetic tree was constructed by using the maximum-likelihood method PhyML 3.0 (12) with 1,000 pseudoreplicates and, in parallel, Bayesian Markov chain Monte Carlo tree-sampling methods by using MrBayes 3.1.2 (13). The Akaike information criterion was chosen as the model selection framework, and the Johnes-Taylor-Thorton model of sequence evolution with gamma-distributed rate variation among sites (JJT + Γ) was chosen as the best model. Maximum-likelihood bootstrap replicate scores (>70%) and posterior probabilities of the Bayesian analysis (>90%) are shown next to the nodes. Taxon information includes strain designation, GenBank accession number, country of origin, and year of detection/isolation. Boldface indicates the Usutu-BONN strain. Scale bar indicates amino acid substitutions per site.

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