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Volume 22, Number 1—January 2016
Letter

New Clinical Strain of Neisseria gonorrhoeae with Decreased Susceptibility to Ceftriaxone, Japan

Takashi Deguchi1Comments to Author , Mitsuru Yasuda1, Kyoko Hatazaki1, Koji Kameyama, Kengo Horie, Taku Kato, Kohsuke Mizutani, Kensaku Seike, Tomohiro Tsuchiya, Shigeaki Yokoi, Masahiro Nakano, and Mutsumasa Yoh
Author affiliations: Gifu University, Gifu, Japan (T. Deguchi, M. Yasuda, K. Hatazaki, K. Kameyama, K. Horie, T. Kato, K. Mizutani, K. Seike, T. Tsuchiya, S. Yokoi, M. Nakano); Yoh Clinic, Inazawa, Japan (M. Yoh)

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Figure

Sequences of altered amino acids in penicillin-binding protein 2 (PBP2) of Neisseria gonorrhoeae strains with decreased susceptibility to oral cephalosporins and strains with resistance to ceftriaxone. Strain GU140106 was isolated from a urethral swab sample from a man in in Nagoya, Japan, who had received fellatio, without condom use, from a female sex worker. Sequences are aligned with wild-type PBP2 derived from nucleic acid sequence of the penA gene of penicillin-susceptible N. gonorrhoeae s

Figure. Sequences of altered amino acids in penicillin-binding protein 2 (PBP2) of Neisseria gonorrhoeae strains with decreased susceptibility to oral cephalosporins and strains with resistance to ceftriaxone. Strain GU140106 was isolated from a urethral swab sample from a man in in Nagoya, Japan, who had received fellatio, without condom use, from a female sex worker. Sequences are aligned with wild-type PBP2 derived from nucleic acid sequence of the penA gene of penicillin-susceptible N. gonorrhoeae strain LM306 (GenBank accession no. M32091). The PBP2 pattern X of strains with decreased susceptibility to oral cephalosporins is quoted from our previous study (6). The PBP2s of ceftriaxone-resistant strains H041 and F89 are derived from their penA genes (GenBank accession nos. AB546858 and JQ073701, respectively). The PBP2 of strain A8806, which has decreased susceptibility to ceftriaxone, is derived from the nucleic acid sequence of the penA gene (David M. Whiley, pers. comm., 2015). In strain H041, the concurrent presence of substitutions A311V, V316P, and T483S was reported to be responsible for conferring resistance to ceftriaxone (7). The PBP2 of strain GU140106 is derived from the nucleic acid sequence of the penA gene (GenBank accession no. LC056026); the strain has the same A311V and T483S substitutions as strain H041, but it has substitution V316T instead of V316P. The PBP2s of strains GU140106 and A8806 have the same amino acid substitutions at A311, V316, and T483. In addition, PBP2 of strain GU140106 has several amino acid changes in positions 227–281 that were not observed in other strains.

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1These authors contributed equally to this article.

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