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Volume 22, Number 1—January 2016
Dispatch

Legionnaires’ Disease in South Africa, 2012–2014

Nicole WolterComments to Author , Maimuna Carrim, Stefano Tempia, Cheryl Cohen, Sibongile Walaza, Philip Sahr, Linda de Gouveia, Florette K. Treurnicht, Orienka Hellferscee, Adam L. Cohen, Alvaro J. Benitez, Halima Dawood, Ebrahim Variava, Jonas M. Winchell, and Anne von Gottberg
Author affiliations: National Institute for Communicable Diseases, Johannesburg, South Africa (N. Wolter, M. Carrim, C. Cohen, S. Tempia, S. Walaza, P. Sahr, L. de Gouveia, F. Treurnicht, O. Hellferscee, A. von Gottberg); University of the Witwatersrand, Johannesburg (N. Wolter, M. Carrim, C. Cohen, S. Walaza, L. de Gouveia, A. von Gottberg); US Centers for Disease Control and Prevention, Pretoria, South Africa (S. Tempia); University of Pretoria, Pretoria (P. Sahr); US Centers for Disease Control and Prevention, Atlanta, Georgia, USA (A.L. Cohen, A.J. Benitez, J.M. Winchell); Pietermaritzburg Metropolitan Hospitals, Pietermaritzburg, South Africa (H. Dawood); University of KwaZulu-Natal, Pietermaritzburg (H. Dawood); Klerksdorp-Tshepong Hospital Complex, Klerksdorp, South Africa (E. Variava)

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Abstract

During June 2012–September 2014, we tested patients with severe respiratory illness for Legionella spp. infection and conducted a retrospective epidemiologic investigation. Of 1,805 patients tested, Legionella was detected in samples of 21 (1.2%); most were adults who had HIV or tuberculosis infections and were inappropriately treated for Legionella.

Data are limited regarding prevalence of Legionella spp. bacteria that cause community-acquired pneumonia (CAP) in Africa (1), despite the high prevalence of HIV-infected adults in many African countries, including South Africa (2). Legionellosis is a notifiable disease in South Africa but is rarely reported. We sought to determine the prevalence of Legionella spp. infections in South Africa and describe epidemiologic characteristics of patients with Legionnaires’ disease (LD).

The Study

During June 2012–September 2014, we conducted a prospective, hospital-based, observational study as part of the severe respiratory illness (SRI) surveillance at 2 sites in South Africa: Klerksdorp-Tshepong Hospital Complex, Klerksdorp, North West Province; and Edendale Hospital, Pietermaritzburg, KwaZulu-Natal Province. A patient with SRI was defined as a person hospitalized with lower respiratory tract infection of any duration. We used a standardized questionnaire to collect demographic and clinical information. Nasopharyngeal specimens and induced sputum samples were tested for Legionella spp. infections by using a real-time PCR assay, as previously described (3). Specimens that were Legionella positive were also tested by real-time PCR assays to identify L. pneumophila and L. longbeachae. In addition, patients’ specimens were tested for other respiratory pathogens and for HIV. Of the 22 Legionella-positive patients, we could trace 17 with whom we conducted a retrospective epidemiologic investigation, which included interviews (detailed study methods in the Technical Appendix).

During June 2012–September 2014, a total of 4,525 SRI patients were enrolled; induced sputum specimens, the recommended specimen type for Legionella spp. detection, were collected from 1,805 (40%). Of 1,803 patients with sputum specimens for which data were available, 885 (49%) were male, and 324 (18%) were children <5 years of age. HIV prevalence was 64% (1,025 of 1,594 patients with sputum specimens and known HIV status), and prevalence of active tuberculosis (TB) infection was 24% (421 of 1,758 patients with sputum specimens and known TB status). Of 1,720 patients with sputum specimens and known survival status, 142 (8%) patients died.

Figure 1

Thumbnail of Number of case-patients and detection rate for Legionella spp. infections, by age group, South Africa, June 2012–September 2014 (N = 1,803).

Figure 1. Number of case-patients and detection rate for Legionella spp. infections, by age group, South Africa, June 2012–September 2014 (N = 1,803).

Among the 1,805 patients with sputum samples, 21 (1.2%, 95% CI 0.7%–1.7%) tested positive for Legionella spp. by real-time PCR. For 1 patient (designated E1 in the online Technical Appendix Table) from whom sputum could not be collected, Legionella spp. infection was detected in the nasopharyngeal specimen, so 22 patients with Legionella spp. infections were detected in total. Among the 21 patients whose sputum tested positive for Legionella spp. infections, median age was 40 years (range 19–59 years; Figure 1), and 11 (52%) were males.

Figure 2

Thumbnail of Number of case-patients and detection rate of Legionella spp. infections, by month and year, for Edendale Hospital and Klerksdorp-Tshepong Hospital Complex, South Africa, June 2012–September 2014 (N = 1,805). \

Figure 2. Number of case-patients and detection rate of Legionella spp. infections, by month and year, for Edendale Hospital and Klerksdorp-Tshepong Hospital Complex, South Africa, June 2012–September 2014 (N = 1,805). \

A cluster of case-patients (15/21 [71%]) was observed during July–December 2012 (Figure 2), including all 6 from Edendale Hospital and 10 (10/16, 63%) from Klerksdorp-Tshepong Hospital Complex. These sites are geographically distant (≈600 km) from one another, so the respective clusters or outbreaks are unlikely to be related. We did not culture samples with Legionella spp. infection, so we were unable to perform strain typing to confirm whether the clusters were caused by related strains. The remaining 6 patients from Klerksdorp-Tshepong Hospital Complex appeared to have sporadic infections.

Legionella patients resided in different areas or communities within the cities of Pietermaritzburg and Klerksdorp. Epidemiologic investigation revealed exposure to several potential sources of infection, such as waste management, air conditioners, plumbing, mining, and swimming pools; however, no common exposure could be identified, so environmental sampling and testing were not performed.

Fifteen (75%) of 20 Legionella patients with known HIV status were infected with HIV, and 9 (43%) of the 21 patients tested positive for TB at the admission during which Legionella infection was detected. HIV or TB infection, or both, was detected in 18 (90%) of 20 patients with known HIV and TB status. A history of active TB before the admission during which Legionella was detected was reported for 14 (82%) of 17 patients. For 17 Legionella spp.–infected patients for whom information was available, additional LD-associated factors included regular alcohol consumption (10 [59%]), cigarette smoking (9 [53%]), asthma (2 [12%]), and heart disease (2 [12%]).

Eighteen (86%) of 21 patients had symptoms >7 days before hospital admission, a delay possibly occurring because many patients were chronically ill (75% were HIV infected and >43% had TB). Median duration of hospitalization for Legionella patients was 4 days (range 1–35 days), and 1 (9%) patient was admitted to intensive care and survived the illness; 4 (20%) patients died. Antimicrobial drug treatment (in-hospital and discharge medication) was known for 21 patients and included amoxicillin/clavulanic acid (16 [76%]), anti-TB medications (15 [71%]), cotrimoxazole (7 [33%]), cefuroxime/ceftriaxone (5 [24%]), and erythromycin (5 [24%]).

Legionella spp. isolates were identified for 2 patients as L. pneumophila serogroup 1 and L. longbeachae. Species could not be determined for 19 patients because of low bacterial loads in their specimens. Of the 21 patients with Legionella-positive sputum specimens, co-infections were detected in 14 (67%). Co-infecting pathogens were Mycobacterium tuberculosis (9 [43%]), rhinovirus (6 [29%]), respiratory syncytial virus (2 [10%]), adenovirus (2 [10%]), Bordetella pertussis (1 [5%]), and Streptococcus pneumoniae (1 [5%]).

Legionella spp. detection rates in this study were similar to those described in other countries (4). However, age distribution tended toward younger adults, not the elderly, the population previously reported as most affected (4). Men and women were evenly distributed in our study, although a substantial male predominance is common for LD (2,4). Differences in age and gender distributions, compared with distributions in other studies, likely result from high HIV and TB prevalence among younger adults in our study population. LD is typically associated with summer because warm and wet conditions promote bacterial replication (2,4). Longer periods of surveillance are needed to establish seasonality of LD in South Africa.

Clinically, patients with LD in this study were likely to be HIV-infected, chronically ill persons with suspected or confirmed TB and were therefore usually treated for TB infection and discharged. HIV-induced immune suppression and lung damage because of biologic or chemical agents likely increased their susceptibility to Legionella infections. Cases of LD and TB occurring simultaneously have been previously described (57). Legionella infection in populations with HIV or TB co-infections may cause acute exacerbation of respiratory symptoms, prompting patients to seek hospital care.

In South Africa, treatment for CAP is usually penicillin or ampicillin for adults <65 years of age and amoxicillin/clavunate or cefuroxime for elderly or HIV-infected adults (8). However, treatment for LD should include a macrolide or fluoroquinolone (4). Only one fourth of Legionella patients in this study received appropriate treatment, likely because of clinical inability to distinguish LD from other forms of pneumonia and because clinicians rarely consider Legionella when they lack access to diagnostic testing and local prevalence data. This problem is further compounded by the high prevalence of HIV and TB in South Africa. Anti-TB treatment, which was administered to more than two thirds of the Legionella patients, would have had therapeutic benefits; rifampin has been shown to have activity against Legionella spp. (9,10). However, suboptimal treatment of Legionella patients with co-infections likely contributed to a case-fatality ratio (20%) more than twice that for all SRI patients (8%) (4,11). Lack of appropriate treatment of patients with CAP in South Africa for atypical pathogens has been described (12).

Conclusions

In South Africa, patients with LD often have chronic illness caused by co-infections such as HIV and TB at time of admission. Legionella infections in most patients were undiagnosed, and patients were suboptimally treated for TB or more typical causes of CAP. Increased awareness and improved diagnostic testing could result in earlier diagnosis, appropriate treatment, and improved outcomes for these patients. In addition to routine diagnostics, surveillance for LD should be performed on an ongoing basis for rapid identification and response to outbreaks.

Dr. Wolter is a senior medical scientist in the Centre for Respiratory Diseases and Meningitis at the National Institute for Communicable Diseases in Johannesburg and is a specialist in molecular microbiology. Her research interests include the diagnosis and epidemiology of respiratory pathogens.

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Acknowledgments

We thank Iain Kennedy, who assisted with the epidemiologic investigation, and Makatisane Papo for his help with data management. We also thank all members involved in the Severe Acute Respiratory Illness study for the collection of specimens and data management.

This work was supported by the National Health Laboratory Service, South Africa, and the US Centers for Disease Control and Prevention (CDC-RFA-GH12-00403). A. von G. received grants from the US Centers for Disease Control and Prevention and Pfizer-South Africa. H.D. received honoraria or travel grants from Merck Sharp & Dohme–South Africa; Novartis–South Africa; and Pfizer–South Africa.

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References

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DOI: 10.3201/eid2201.150972

Table of Contents – Volume 22, Number 1—January 2016

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Nicole Wolter, Centre for Respiratory Diseases and Meningitis, National Institute for Communicable Diseases, Private Bag X4, Sandringham, 2131, Gauteng, South Africa

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Page created: December 18, 2015
Page updated: December 18, 2015
Page reviewed: December 18, 2015
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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