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Volume 22, Number 11—November 2016
CME ACTIVITY - Synopsis

Transmission of Babesia microti Parasites by Solid Organ Transplantation

Meghan B. BrennanComments to Author , Barbara L. Herwaldt, James J. Kazmierczak, John W. Weiss, Christina L. Klein, Catherine P. Leith, Rong He, Matthew J. Oberley, Laura Tonnetti, Patricia P. Wilkins, and Gregory M. Gauthier
Author affiliations: University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA (M.B. Brennan, J.W. Weiss, C.L. Klein, C.P. Leith, R. He, M.J. Oberley, G.M. Gauthier); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (B.L. Herwaldt, P.P. Wilkins); Wisconsin Division of Public Health, Madison (J.J. Kazmierczak); American Red Cross Badger–Hawkeye Blood Service Region, Madison (J.W. Weiss); American Red Cross Jerome H. Holland Laboratories for the Biomedical Sciences, Rockville, Maryland, USA (L. Tonnetti)

Cite This Article

Introduction

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Medscape, LLC designates this Journal-based CME activity for a maximum of 1.00 AMA PRA Category 1 Credit(s)™. Physicians should claim only the credit commensurate with the extent of their participation in the activity.

All other clinicians completing this activity will be issued a certificate of participation. To participate in this journal CME activity: (1) review the learning objectives and author disclosures; (2) study the education content; (3) take the post-test with a 75% minimum passing score and complete the evaluation at http://www.medscape.org/journal/eid; and (4) view/print certificate.

Release date: October 12, 2016; Expiration date: October 12, 2017

Learning Objectives

Upon completion of this activity, participants will be able to:

•     Identify the presentation and likely mode of transmission of babesiosis after organ transplantation, based on 2 case reports

•     Determine treatment of babesiosis in patients receiving organ transplantation

•     Assess the clinical implications of findings from these case reports of babesiosis.

CME Editor

Thomas J. Gryczan, MS, Technical Writer/Editor, Emerging Infectious Diseases. Disclosure: Thomas J. Gryczan, MS, has disclosed no relevant financial relationships.

CME Author

Laurie Barclay, MD, freelance writer and reviewer, Medscape, LLC. Disclosure: Laurie Barclay, MD, has disclosed the following relevant financial relationships: owns stock, stock options, or bonds from Pfizer.

Authors

Disclosures: Meghan B. Brennan, MD, MS; Barbara L. Herwaldt, MD, MPH; James J. Kazmierczak, DVM, MS; John W. Weiss, MD, PhD; Catherine P. Leith, MB BChir; Rong He, MD; Laura Tonnetti, PhD; Patricia P. Wilkins, PhD; and Gregory M. Gauthier, MD, MS, have disclosed no relevant financial relationships. Christina L. Klein, MD, has disclosed the following relevant financial relationships: served as an advisor or consultant for Alexion Pharmaceuticals; served as a speaker or a member of a speakers bureau for Alexion Pharmaceuticals. Matthew J. Oberley, MD, PhD, has disclosed the following relevant financial relationships: served as an advisor or consultant for Amgen; owns stock, stock options, or bonds from Novartis.

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Abstract

Babesia microti, an intraerythrocytic parasite, is tickborne in nature. In contrast to transmission by blood transfusion, which has been well documented, transmission associated with solid organ transplantation has not been reported. We describe parasitologically confirmed cases of babesiosis diagnosed ≈8 weeks posttransplantation in 2 recipients of renal allografts from an organ donor who was multiply transfused on the day he died from traumatic injuries. The organ donor and recipients had no identified risk factors for tickborne infection. Antibodies against B. microti parasites were not detected by serologic testing of archived pretransplant specimens. However, 1 of the organ donor’s blood donors was seropositive when tested postdonation and had risk factors for tick exposure. The organ donor probably served as a conduit of Babesia parasites from the seropositive blood donor to both kidney recipients. Babesiosis should be included in the differential diagnosis of unexplained fever and hemolytic anemia after blood transfusion or organ transplantation.

Babesia microti, an intraerythrocytic parasite, is the most common cause of human babesiosis in the United States and is endemic to the Northeast and upper Midwest regions, including parts of Wisconsin and Minnesota (14). B. microti infection can range from asymptomatic to severe. Common manifestations include hemolytic anemia and nonspecific influenza-like symptoms (2). Persons who are asplenic, elderly, or immunocompromised are at increased risk for symptomatic infection and for severe complications, such as multiorgan dysfunction and death (5).

The primary route of transmission of B. microti parasites is by the bite of an infected Ixodes scapularis tick (6). Transmission of Babesia parasites by blood transfusion also is well-documented (711). In contrast, transmission associated with solid organ transplantation has not been reported. We investigated 2 cases of babesiosis for which transmission probably occurred when renal allografts were transplanted from a multiply transfused organ donor.

Materials and Methods

Diagnosis of babesiosis in 2 persons who received kidney transplants from the same donor prompted multifaceted, collaborative investigations, which were conducted by the authors and acknowledged persons and agencies (e.g., transplant, transfusion, and public health organizations). The Organ Procurement Organization (Madison, WI, USA) identified the disposition of all organs and tissues recovered from the organ donor and notified the United Network for Organ Sharing (Richmond, VA, USA) about the possibility of donor-derived transmission.

Only kidneys and corneas had been transplanted; the bilateral iliac arteries and veins had been recovered but discarded 14 days later, whereas the liver and other tissues that had been donated for research were embargoed. Medical and transfusion records of the organ donor and transplant recipients were reviewed, as were procedures and records for organ/tissue recovery, handling, and transplantation. The transplant recipients, the seropositive blood donor identified in the transfusion investigation, and surrogates for the organ donor were interviewed regarding risk factors for and potential clinical manifestations of Babesia infection.

Specimens from the transplant recipients, the organ donor, and the organ donor’s blood donors were tested for evidence of Babesia infection. Evaluations of the transplant recipients included light microscopy of Giemsa- or Wright-stained thick and thin blood smears for Babesia parasites. The Centers for Disease Control and Prevention (CDC; Atlanta, GA, USA) conducted reference diagnostic testing of specimens from transplant recipients and organ donor. CDC also conducted serologic testing by using an indirect fluorescent antibody (IFA) assay for total immunoglobulin against B. microti antigens (12). Serum and plasma specimens were tested in serial 4-fold dilutions, and a reciprocal dilution titer of 64 was considered positive. CDC conducted PCR analysis of whole-blood specimens from the transplant recipients by using primers specific for the B. microti 18S rRNA gene (13) and a previously described 2-step nested PCR (7). CDC also conducted B. microti PCR analysis of fresh-frozen hepatic tissue from the organ donor. No fresh-frozen renal tissue or whole-blood specimens from the organ donor were available for testing. However, paraffin-embedded, pretransplantation specimens from both kidneys were available and were tested by using a B. microti immunohistochemical (IHC) assay (14); CDC also conducted IHC testing of hepatic tissue.

The American Red Cross obtained blood/serum specimens from all 33 blood donors who had contributed components transfused into the organ donor. No segments or components from original donor units were available for testing. The American Red Cross tested postdonation specimens by using a B. microti IFA assay for IgG and a B. microti real-time PCR. IFA testing was conducted with serial 2-fold dilutions of samples.

Case Reports

Renal Transplant Recipients

In late August 2008, two men with end-stage diabetic nephropathy (a 65-year-old Wisconsin resident [patient A; the index case-patient] and a 41-year-old Iowa resident [patient B]) received renal allografts from the same deceased donor at the University of Wisconsin Hospital and Clinics (UWHC; Madison, WI, USA). Different surgeons in separate operating rooms transplanted the kidneys. Both patients received induction immunosuppressive therapy with basiliximab and maintenance therapy with prednisone, mycophenolate mofetil, and tacrolimus. During the previous year and peritransplant period, neither patient lived or traveled in babesiosis-endemic regions, which in the Midwest, included parts of Minnesota and Wisconsin but not Iowa (Table), and they did not receive blood transfusions.

Figure 1

Thumbnail of Timelines showing key clinical and laboratory events for 2 renal transplant recipients (patients A and B) infected with Babesia microti parasites, Wisconsin, USA, 2008. Trauma, transfusions, death, and organ procurement for the organ donor all occurred on the same day in late August 2008. NPF, no parasites were found by examination of thick and thin blood smears.

Figure 1. Timelines showing key clinical and laboratory events for 2 renal transplant recipients (patients A and B) infected with Babesia microti parasites, Wisconsin, USA, 2008. Trauma, transfusions, death, and organ procurement for...

Both patients showed seroconversion and development of parasitologically confirmed cases of babesiosis, which were diagnosed ≈8 weeks posttransplantation (Table; Figure 1). At the request of the transplant physicians, both patients were evaluated by the same UWHC infectious disease specialists.

After babesiosis was diagnosed, doses of immunosuppressive medications were decreased and each patient received a 6-week course of oral antimicrobial drug therapy: atovaquone (750 mg, 2×/d for 6 wks) plus azithromycin (1,000 mg, 1×/d for 2 wks, followed by 600 mg, 1×/d for 4 wks). During therapy, symptoms resolved, laboratory parameters returned to reference ranges or values, and Babesia parasite DNA became undetectable (Table; Figure 1).

Patient A

On October 2, 2008 (≈5 weeks posttransplantation), during a routine follow-up appointment at the UWHC Transplant Clinic, the wife of patient A mentioned that he had a lack of energy and decreased appetite (onset date not specified). At that clinic visit, his hematocrit was 37%, which approximated his baseline value posttransplantation.

On October 8, he was admitted to the UWHC, as planned, to have his peritoneal dialysis catheter removed the next day. However, at admission, he unexpectedly was found to have a temperature of 39.4°C. His hematocrit values were 33% and 28% on October 8 and 9, respectively. Removal of the catheter was postponed until October 10, and he was discharged after the procedure. Cultures of the catheter tip, blood, and urine specimens were negative for bacterial growth. He was treated empirically with piperacillin/tazobactam during his 2-day hospitalization, followed by a 7-day outpatient course of ciprofloxacin.

Figure 2

Thumbnail of Wright-stained peripheral blood smear from patient A (index case-patient), a renal transplant recipient infected with Babesia microti parasites, Wisconsin, USA, 2008. The smear shows intraerythrocytic Babesia parasites, a ring form (black arrow), and a Maltese cross or tetrad form (red arrow), which is pathognomonic for babesiosis. Scale bar indicates 10 μm.

Figure 2. Wright-stained peripheral blood smear from patient A (index case-patient), a renal transplant recipient infected with Babesia microti parasites, Wisconsin, USA, 2008. The smear shows intraerythrocytic Babesia parasites, a ring form (black...

On October 16 (day 6 of ciprofloxacin therapy), his wife called the transplant coordinator to report that he had a low-grade temperature (37.5°C) and a 2-day history of drenching sweats. An appointment in the Transplant Clinic was scheduled for October 20 to evaluate his symptoms. During the appointment, he reported a several-day history of darkening urine and progressive fatigue since his previous hospitalization. Per routine for clinic visits, a complete blood count was determined. His hematocrit had decreased to 21% (Table). Because platelet clumping was detected by using an automated hematology analyzer, a blood smear was reviewed manually: intraerythrocytic Babesia parasites were visualized at a parasitemia level of 8% (Table; Figure 2). On the same day (October 20), he was admitted to the UWHC, evaluated by the Infectious Disease Service, and began treatment with azithromycin plus atovaquone (Table; Figure 1). Within 48 hours of initiating therapy, his appetite and exercise tolerance increased, and his parasitemia level decreased to <5%.

Patient B

During October 4–9, patient B was hospitalized in Iowa for evaluation of epigastric discomfort, dyspepsia, nausea, and low-grade fever of unclear etiology. His hemoglobin level was 12.1 g/dL. Computed tomography (CT) imaging of his abdomen and pelvis was unremarkable except for enlargement of the pancreatic head (amylase and lipase values were within reference ranges). While hospitalized, he was treated empirically with metronidazole and levofloxacin; a 7-day outpatient course of ciprofloxacin therapy was prescribed.

Figure 3

Thumbnail of Computed tomography (CT) scan of the abdomen of patient B, a renal transplant recipient infected with Babesia microti parasites, Wisconsin, USA, 2008. Taken on November 5, the scan shows a splenic infarction (white arrow) that had not been visualized on a CT scan on October 5. Although the cause of the splenic infarction was not determined, the infarction might have been a complication of babesiosis, as reported for other patients (16,17).

Figure 3. Computed tomography (CT) scan of the abdomen of patient B, a renal transplant recipient infected with Babesia microti parasites, Wisconsin, USA, 2008. Taken on November 5, the scan shows a splenic...

On October 23, during a routine follow-up appointment in the UWHC Transplant Clinic, he was afebrile but, on prompting, recalled a transient fever (38°C) ≈1 week earlier. In addition, he reported a several-week history of left upper quadrant pain. At examination, he had tenderness to deep palpation of the left upper quadrant, which worsened with deep inspiration. A manual (nonautomated) review of a blood smear was requested explicitly, prompted by diagnosis of the case of babesiosis in patient A 3 days earlier. Intraerythrocytic Babesia parasites also were observed on the blood smear for patient B; the parasitemia level was 1%. His hemoglobin level was 11 g/dL, and his hematocrit was 35%. On the same day, he was evaluated in the UWHC Infectious Disease Clinic and began outpatient therapy with atovaquone plus azithromycin. To evaluate his abdominal pain, CT of the abdomen and pelvis was performed on an outpatient basis (November 5); it showed a splenic infarction (Figure 3), which was not detected by CT in early October. During the course of antimicrobial drug therapy, his abdominal pain and constitutional symptoms resolved.

Organ Donor

The organ donor was a 22-year-old man who was a resident of an urban area of Wisconsin to which babesiosis was not endemic. According to his relatives and primary care physician, he had been in good health and did not have any potentially relevant travel or clinical manifestations during the previous year. His only known risk factor for exposure to Babesia parasites was receipt of multiple blood transfusions during resuscitation attempts on the day he died from unintentional trauma. Although an autopsy was not performed, a limited number of plasma, serum, and tissue specimens were available for Babesia testing. Antibodies against B. microti parasites were not detected by retrospective serologic testing of a pretransfusion plasma specimen and 2 posttransfusion serum specimens (IFA titer <8). Tissue sections from both kidneys had negative IHC results. IHC testing of hepatic tissue showed a few rare foci of suspicious staining but no definitive evidence of Babesia parasites, and hepatic tissue showed negative results by PCR.

The cornea recipients were contacted, and blood specimens collected ≈3–4 months posttransplantation were tested for evidence of Babesia infection. Specimens showed negative results for PCR and IFA analysis, and no parasites were found on blood smears.

Transfusion Investigation

During resuscitation attempts, the organ donor received 20 cellular blood components (19 units of erythrocytes and 1 unit of apheresis platelets) and 13 plasma units. Only 1 of the 33 donors, a 52-year-old man, had evidence of B. microti infection. Specimens available for testing were collected 88 and 151 days postdonation and had IFA titers of 256 and 128, respectively; both specimens showed negative results by PCR. The seropositive blood donor was the source of 1 of the organ donor’s last erythrocyte transfusions, which was transfused 15 days postdonation.

This blood donor lived in a babesiosis-endemic area of Minnesota (Washington County) and had camped in disease-endemic areas in northern Wisconsin (Ashland County) in May 2008 and in northern Minnesota (Saint Louis County) in July 2008. During the retrospective investigation, he recalled a fever (39.4°C), chills, and diaphoresis, which lasted ≈36 hours, during the first week of June. Although he did not recall any tick bites, his wife reportedly had found a tick on his body (timing and other details not specified). No cellular components from his donation in August were transfused to other patients. After he was found to be seropositive, he was deferred indefinitely from future blood donations. However, he already had donated blood in the interim (in September 2008), and apheresis platelets from the donation had been transfused. A specimen obtained ≈2 months posttransfusion from the platelet recipient was tested in a commercial laboratory and showed negative B. microti IFA and PCR results.

Discussion

We investigated parasitologically confirmed cases of babesiosis in 2 recipients of renal allografts from an organ donor whose only known risk factor for exposure to Babesia parasites was the receipt of multiple blood transfusions on the day he died. The organ donor and the kidney recipients did not have antibodies against B. microti parasites detected by retrospective testing of pretransplantation specimens. However, 1 of the organ donor’s blood donors was seropositive when tested postdonation and had risk factors for tick exposure.

The most likely scenario is that the kidney donor served as a conduit of Babesia parasites from this blood donor to the kidney recipients (i.e., the blood donor became infected by tickborne transmission, secondary transmission occurred by erythrocyte transfusion, and tertiary transmission occurred by organ transplantation). The possibility that the kidney recipients became infected independently is remote: they did not live, travel, or receive medical care in any known babesiosis-endemic areas in the Midwest or elsewhere; they did not receive any transfusions; and they showed seroconversion posttransplantation, despite being immunosuppressed. Although no subtyping tools are available to establish that the patients were infected with the same B. microti strain, they almost assuredly became infected from the same source at approximately the same time.

Previous reports have described organ transplant recipients who became infected with Babesia parasites by tickborne- or transfusion-associated transmission in the peritransplant period or thereafter (10,1822). Transplantation-associated transmission of B. microti parasites, which are not known to have an exoerythrocytic tissue phase, has not been described, nor has the occurrence of 3 consecutive routes of transmission (vector, transfusion, and transplantation), which has been reported for West Nile virus (23,24). The plausibility of transplantation-associated transmission of B. microti parasites, in the context of residual parasites in the renal vasculature/fluids after flushing the organs, is supported in part by data from other contexts (e.g., transfusion-associated cases) that suggest low inocula of the parasite can cause infection (10,25).

Although we do not have proof that the blood donor was infected when he donated blood or have laboratory evidence that the organ donor briefly harbored the parasite, the negative PCR results for the postdonation specimens from the blood donor and the negative PCR and IHC results for the available posttransfusion specimens from the organ donor are not helpful; only positive results would have been informative. Although other transmission scenarios seem much less probable, the cases of babesiosis in the kidney recipients we report would be noteworthy even if the organ donor recently had acquired the parasite from a tick (i.e., was in the early window period of infection, despite his lack of known risk factors for tickborne transmission).

Diagnosis of babesiosis in the kidney recipients prompted multiagency investigations of the organ donor and his blood donors. However, the cases of babesiosis in the recipients could have been easily missed, which highlights the possibility that other transplantation-associated cases have occurred but were not diagnosed or investigated. For patient A (index case-patient), babesiosis was diagnosed because of the serendipitous finding of parasites on a blood smear that was examined manually because of platelet clumping. His lack of risk factors for tickborne transmission and the possibility of donor-derived infection led to prompt evaluation of patient B. At the time of diagnosis, illness in patient B was milder (lower-level parasitemia, minimal anemia, and transient fever) than that in patient A, even though the 2 patients received similar immunosuppressive regimens.

Babesiosis can be persistent, relapsing, or life threatening in immunocompromised patients (18,19,22,2628). Optimal therapy for babesiosis in patients who have received an organ transplant or have impaired immunity for other reasons is not well established and might depend on multiple factors; a uniform recommendation might not be applicable to such a heterogeneous population. In immunocompetent persons, the typical duration of antimicrobial drug therapy for babesiosis is 7–10 days (6). We decided to treat both kidney recipients for 6 weeks on the basis of retrospective data for immunosuppressed patients that suggest the likelihood of cure is higher if combination antimicrobial drug therapy is administered for >6 weeks, including 2 weeks after Babesia parasites are no longer detected on blood smears (27). We gave the patients atovaquone plus azithromycin rather than clindamycin plus quinine (the standard of care for severely ill patients [6,29]) to minimize the likelihood of toxicity during their 6-week treatment courses. In addition, we decreased the doses of their immunosuppressive medications.

Both patients tolerated and responded well to the antimicrobial treatment, without documented relapses. However, clinicians should be aware that clinical resistance reportedly developed in several immunosuppressed patients treated for prolonged periods with atovaquone plus azithromycin (28); whether particulars of those patients’ treatment regimens (e.g., antimicrobial drug dosing) contributed to development of clinical resistance is not known (28,30).

The cases of babesiosis we describe not only underscore the plausibility and likelihood of transmission by organ transplantation, but also highlight the emerging role of transfusion-associated babesiosis. For the 3-decade period of 1979 (the year the first known transfusion case occurred) through 2009, a total of 159 transfusion-associated cases of B. microti infection were identified in the United States, most (77%) of which occurred during 2000–2009 (10). Asymptomatic persons can fulfill all of the criteria for donating blood despite having low-level parasitemia sufficient to cause infection in a transfusion recipient (10).

To date, no Babesia tests for screening US blood donors have been licensed by the Food and Drug Administration, and no pathogen-reduction technologies for cellular blood components have been approved (3135). However, the Blood Products Advisory Committee of the Food and Drug Administration that was convened on May 13, 2015, supported the concepts of year-round B. microti serologic testing of all US blood donors and of B. microti nucleic acid–based testing of donors in selected states (details remain to be determined) (36). Because of donor travels and shipments/distributions of blood components, transmission by transfusion is not limited to babesiosis-endemic foci (10). For example, the seropositive erythrocyte donor we identified had donated blood in a babesiosis-endemic area of Minnesota. This blood was then transported to and transfused in an area of Wisconsin to which babesiosis was not endemic.

As we described, unrecognized tickborne transmission of Babesia parasites to the blood donor probably led to transmission by transfusion to the organ donor and subsequent transmission by organ transplantation to both kidney recipients. Clinicians should include babesiosis in the differential diagnosis of unexplained fever and hemolytic anemia after blood transfusion or organ transplantation, even in regions to which babesiosis is not endemic. Suspected cases of iatrogenic transmission should be reported to state and local public health authorities. In addition, cases that might be transfusion or transplantation associated should be reported to the pertinent blood center and organ procurement organization, respectively.

Dr Brennan is an infectious disease physician at the University of Wisconsin Hospital and Clinics, Madison, Wisconsin. Her primary research interest is infectious disease health services.

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Acknowledgments

We thank the many persons who contributed to the collaborative investigations, including contributors at the American Red Cross, the Centers for Disease Control and Prevention, the Lions Eye Bank of Wisconsin, the Musculoskeletal Transplant Foundation, the University of Wisconsin Hospital and Clinics, the University of Wisconsin Organ and Tissue Donation, and public health departments in Wisconsin and Minnesota, as well as other health professionals. We also thank the kidney and cornea recipients, the seropositive blood donor, the family of the organ donor, and the healthcare providers of the recipients and donors.

This work was supported by grants (UL1TR000427 and KL2TR000428) from the National Institutes of Health to the Wisconsin Institute for Clinical and Translational Research.

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  36. Food and Drug Administration; Meeting of the Blood Products Advisory Committee. Strategies for implementation of antibody and nucleic acid-based testing for Babesia microti in blood donors, May 13, 2015 [cited 2016 Mar 20]. http://www.fda.gov/AdvisoryCommittees/CommitteesMeetingMaterials/BloodVaccinesandOtherBiologics/BloodProductsAdvisoryCommittee/ucm441228.htm

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Earning CME Credit

To obtain credit, you should first read the journal article. After reading the article, you should be able to answer the following, related, multiple-choice questions. To complete the questions (with a minimum 75% passing score) and earn continuing medical education (CME) credit, please go to http://www.medscape.org/journal/eid. Credit cannot be obtained for tests completed on paper, although you may use the worksheet below to keep a record of your answers. You must be a registered user on Medscape.org. If you are not registered on Medscape.org, please click on the “Register” link on the right hand side of the website to register. Only one answer is correct for each question. Once you successfully answer all post-test questions you will be able to view and/or print your certificate. For questions regarding the content of this activity, contact the accredited provider, CME@medscape.net. For technical assistance, contact CME@webmd.net. American Medical Association’s Physician’s Recognition Award (AMA PRA) credits are accepted in the US as evidence of participation in CME activities. For further information on this award, please refer to http://www.ama-assn.org/ama/pub/about-ama/awards/ama-physicians-recognition-award.page. The AMA has determined that physicians not licensed in the US who participate in this CME activity are eligible for AMA PRA Category 1 Credits™. Through agreements that the AMA has made with agencies in some countries, AMA PRA credit may be acceptable as evidence of participation in CME activities. If you are not licensed in the US, please complete the questions online, print the certificate and present it to your national medical association for review.

Article Title:
Transmission of Babesia microti Parasites by Solid Organ Transplantation

CME Questions

1. Your patient is a 52-year-old man with end-stage diabetic nephropathy who experiences fever and anemia after kidney transplantation. According to the case reports by Brennan and colleagues, which of the following statements about presentation and likely mode of transmission of babesiosis after organ transplantation is correct?

A.         The kidney recipients were diagnosed with babesiosis 1 month after transplantation

B.         The kidney recipients had Babesia microti antibodies detected by serologic testing of archived pretransplant specimens

C.        The organ donor came from a Babesia-endemic region and had a history of tick bite

D.        The organ donor likely served as a conduit of Babesia parasites from a seropositive blood donor to both kidney recipients

2. According to the case reports by Brennan and colleagues, which of the following statements about treatment of babesiosis in these patients is correct?

A.         Typical duration of antimicrobial therapy is 2 to 3 weeks in immunocompetent persons

B.         In immunosuppressed patients, cure is more likely with combination antimicrobials for at least 6 weeks, including 2 weeks after parasites clear from blood smear

C.        The recipients were treated with clindamycin plus quinine

D.        One patient experienced clinical resistance during antibiotic therapy and had a relapse of babesiosis

3. According to the case reports by Brennan and colleagues, which of the following statements about the clinical implications of findings from these cases of babesiosis is correct?

A.         Clinicians should consider babesiosis in the differential diagnosis of unexplained fever and hemolytic anemia after transfusion or transplantation only in regions where babesiosis is endemic

B.         Suspected cases of transmission after transfusion or transplantation should be reported only to state and local public health authorities

C.        The US Food and Drug Administration’s (FDA’s) Blood Products Advisory Committee supports the concept of year-round B. microti serologic testing of all US blood donors and nucleic acid–based testing of donors in certain states

D.        The FDA has licensed Babesia tests for screening US blood donors

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3. The content learned from this activity will impact my practice.

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4. The activity was presented objectively and free of commercial bias.

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Cite This Article

DOI: 10.3201/eid2211.151028

Related Links

Table of Contents – Volume 22, Number 11—November 2016

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Please use the form below to submit correspondence to the authors or contact them at the following address:

Meghan B. Brennan, University of Wisconsin Medical Foundation Centennial Bldg, 5th Fl, 1685 Highland Ave, Madison, WI 53705, USA

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Page created: October 12, 2016
Page updated: October 17, 2016
Page reviewed: October 17, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
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