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Volume 22, Number 11—November 2016
Research

Reassortant Eurasian Avian-Like Influenza A(H1N1) Virus from a Severely Ill Child, Hunan Province, China, 2015

Wenfei Zhu1, Hong Zhang1, Xingyu Xiang1, Lili Zhong, Lei Yang, Junfeng Guo, Yiran Xie, Fangcai Li, Zhihong Deng, Hong Feng, Yiwei Huang, Shixiong Hu, Xin Xu, Xiaohui Zou, Xiaodan Li, Tian Bai, Yongkun Chen, Zi Li, Junhua LiComments to Author , and Yuelong ShuComments to Author 
Author affiliations: National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China (W. Zhu, L. Yang, J. Guo, Y. Xie, X. Zou, X. Li, T. Bai, Y. Chen, Z. Li, Y. Shu); Key Laboratory for Medical Virology, National Health and Family Planning Commission, Beijing (W. Zhu, L. Yang, J. Guo, Y. Xie, X. Zou, X. Li, T. Bai, Y. Chen, Z. Li, Y. Shu); Hunan Provincial Center for Disease Control and Prevention, Changsha, China (H. Zhang, X. Xiang, F. Li, Z. Deng, Y. Huang, S. Hu, J. Li); Hunan Provincial People’s Hospital, Changsha (L. Zhong); Liuyang Center for Disease Control and Prevention, Liuyang, China (H. Feng, X. Xu)

Main Article

Table 1

Seroconversion in C57BL/6 mice inoculated with JS/1/11 EA-H1N1 and HuN EA-H1N1 viruses*

Virus and dose, log10 TCID50/50 μL
Hemagglutination inhibition titer†
MID50, log10 TCID50
JS/1/11 EA-H1N1 virus antigen
HuN EA-H1N1 virus antigen
Mouse 1
Mouse 2
Mouse 3
Mouse 4
Mouse 5
Mouse 1
Mouse 2
Mouse 3
Mouse 4
Mouse 5
JS/1/11 EA-H1N1 4.7
6 40 20 >80 40 40 >80 40 >80 40 40
5 <10 40 20 20 40 10 >80 20 40 >80
4 10 10 <10 <10 <10 20 <10 <10 10 <10
3 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
2 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
1
<10
<10
<10
<10
<10

<10
<10
<10
<10
<10

HuN EA-H1N1 2.9
6 ND ND ND ND ND ND ND ND ND ND
5 ND ND ND ND ND ND ND ND ND ND
4 >80 40 >80 >80 <10 >80 >80 >80 >80 <10
3 >80 40 40 <10 20 >80 >80 80 <10 40
2 <10 <10 <10 <10 <10 <10 <10 <10 <10 <10
1
<10
<10
<10
<10
<10

<10
<10
<10
<10
10

PBS <10 <10 <10 <10 <10 <10 <10 <10 <10 <10

*Serum samples were obtained from mice 14 d after inoculation. HuN EA-H1N1, Eurasian avian-like influenza A/Hunan/42443/2015(H1N1) virus; JS/1/11 EA-H1N1, Eurasian avian-like influenza A/Jiangsu/1/2011(H1N1) virus; MID50, mouse infectious dose; ND, not determined due to death of the animal (treated as positive for determination of the MID50); TCID50, 50% tissue culture infectious dose.
†Titers <20 were regarded as negative for seroconversion.
‡MID50 was determined using the Spearman–Karber method (19).

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1These authors contributed equally to this article.

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