Volume 22, Number 11—November 2016
Shigella flexneri with Ciprofloxacin Resistance and Reduced Azithromycin Susceptibility, Canada, 2015
To the Editor: In 2015, a locally acquired, multidrug-resistant Shigella flexneri infection was identified in Montreal, Quebec, Canada, in an HIV-positive man who had sex with men (MSM). In September, the 53-year-old man consulted his physician at an outpatient clinic after experiencing abdominal pain, fatigue, and diarrhea without blood in stools or fever. The week before the symptom onset, although he had not traveled, he had unprotected oral and anal sexual contact in a Montreal bathhouse with a man visiting Canada from an unknown country. The patient did not work in daycare centers or healthcare facilities, and he was not a food handler. He did not have sex during illness.
He was HIV positive and was receiving antiretroviral treatment; recent CD4 cell count was 480 × 106/L, and HIV viral load was <40 copies/mL. S. flexneri was isolated from his culture of a fecal sample, and Neisseria gonorrhoeae, diagnosed by PCR, was found in a throat specimen. The patient did not have a medical record of other past sexually transmitted infections.
Phenotypic identification of the S. flexneri was confirmed at Laboratoire de Santé Publique du Québec (1). Serologic identification, pulsed-field gel electrophoresis (PFGE), and antimicrobial susceptibility testing were performed as described (1). This S. flexneri, serotype 2a pulsovar 21 (a new PFGE combination pattern in the province of Quebec), was resistant to ampicillin, trimethoprim/sulfamethoxazole (TMP/SMX), nalidixic acid, ciprofloxacin, tetracycline, and chloramphenicol. The isolate was also nonsusceptible to azithromycin and amoxicillin/-clavulanic acid, and susceptible to ceftriaxone, cefixime, ertapenem, and gentamicin (Table). The mph(A) gene, which codes for the macrolide 2′-phosphotransferase, tested positive by PCR (1).
On day 10 of diarrhea, the patient was treated with ceftriaxone, 250-mg dose, intramuscularly, followed by cefixime, 800 mg/day, for 5 days; the patient’s condition showed progressive improvement. Two control cultures of fecal specimens were negative 7 and 16 days, respectively, after completion of a regimen of cefixime.
Shigella spp. are transmitted from person-to-person through low inocula of the bacteria, directly or indirectly (1,3–5). In MSM, Shigella spp. are mostly transmitted sexually, with clusters documented in many countries (1,3–5). In Canada and the United States, Shigella isolates have high levels of resistance to ampicillin and TMP/SMX (1,3–6). In adult patients, when antimicrobial drug treatment is indicated, ciprofloxacin and azithromycin are, respectively, the agents of first and second choices for treating Shigella infections (1,3–5).
In the United States, Shigella spp. resistant to at least nalidixic acid and azithromycin have been found in surveillance isolates: 1/293 in 2011 (Shigella spp.), 1/353 in 2012 (S. sonnei), and 1 of 344 in 2013 (S. flexneri) (6). In Illinois and Montana, during September 2014–April 2015, 3 of 5 patients infected with multidrug-resistant S. sonnei (resistant to ampicillin, TMP/SMX, ciprofloxacin, and nalidixic acid and nonsusceptible to azithromycin), identified themselves as MSM, and 2 of these patients had diarrhea for >14 days (3).
Clinical treatment failure has been reported in patients infected with azithromycin-nonsusceptible Shigella isolates treated with this drug (7,8), including 1 of our patients (unpub. data). In a previous study, the mph(A) gene was acquired by 4 of 7 locally acquired Shigella pulse types infecting MSM. This raises concern that reduced Shigella susceptibility to azithromycin is developing rapidly (1). Azithromycin epidemiologic cutoff values for wild- and non–wild-types of S. flexneri and S. sonnei are newly reported by CLSI (8). In recent years, ciprofloxacin-resistant and/or azithromycin-nonsusceptible Shigella spp. acquired during international travel or acquired locally were reported in the United States and in our hospital center (1,3–6; unpub. data). S. flexneri that is resistant to ceftriaxone and ciprofloxacin has been reported in the United States (9). Infections with multidrug-resistant Shigella spp. may be of longer duration and have higher costs (3).
When evaluating patients with diarrhea, physicians should identify risk factors and request bacterial cultures of fecal specimens. Antimicrobial drug susceptibility testing of Shigella isolates is essential for effective antimicrobial drug treatment. Serologic identification and PFGE are essential for epidemiologic purposes for ascertaining clusters or multidrug-resistant Shigella isolates (1,3–5). Patients with Shigella infection should be advised about preventive practices such as frequent handwashing and precautions when handling food and water (3). MSM should use barriers during oral, anal, and genital sex and wash their genitals, anus, and hands before and after sex (1,3–5).
We suggest obtaining 2 control cultures of fecal specimens on days 2 and 3 after the patient completes antimicrobial treatment for infection with multidrug-resistant Shigella spp. Patients should avoid sex during symptomatic infections and wait for 2 negative stool cultures. Montreal public health officials investigated and counselled this patient as they do for every patient with Shigella infections. In Quebec, physicians and microbiology laboratories are notified of Shigella clusters and multidrug-resistant Shigella infections.
To our knowledge, no other ciprofloxacin-resistant and azithromycin-nonsusceptible Shigella flexneri isolates have been documented in the province of Quebec. No PFGE matches to S. flexneri serotype 2a pulsovar 21 have been identified in Canada. Multidrug-resistant Shigella isolates, including those with both resistance to ciprofloxacin and nonsusceptibility to azithromycin, may be underestimated and incidence may be increasing (1,3–5).
We thank Guy Lapierre and the personnel of bacteriology sections of Centre Hospitalier de l’Université de Montréal–Hôpital Saint-Luc and of Laboratoire de Santé Publique du Québec for their assistance.
- Gaudreau C, Barkati S, Leduc JM, Pilon PA, Favreau J, Bekal S. Shigella spp. with reduced azithromycin susceptibility, Quebec, Canada, 2012-2013. Emerg Infect Dis. 2014;20:854–6.
- CLSI. Performance standards for antimicrobial susceptibility testing; 24th informational supplement; no. M100-S. 26th ed. Wayne (PA): CLSI; 2016.
- Centers for Disease Control and Prevention. Ciprofloxacin- and azithromycin-nonsusceptible shigellosis in the United States. CDC Health Alert Network June 4, 2015 [cited 2016 Jan 20]. http://emergency.cdc.gov/han/han00379.asp
- Gaudreau C, Ratnayake R, Pilon PA, Gagnon S, Roger M, Lévesque S. Ciprofloxacin-resistant Shigella sonnei among men who have sex with men, Canada, 2010. Emerg Infect Dis. 2011;17:1747–50.
- Heiman KE, Karlsson M, Grass J, Howie B, Kirkcaldy RD, Mahon B, ; Centers for Disease Control and Prevention (CDC). Notes from the field: Shigella with decreased susceptibility to azithromycin among men who have sex with men - United States, 2002-2013. MMWR Morb Mortal Wkly Rep. 2014;63:132–3.
- Centers for Disease Control and Prevention. National Antimicrobial Resistance Monitoring System. Enteric bacteria 2013. Human isolates final report. [cited 2016 Feb 29]. http://www.cdc.gov/narms/reports/
- Boumghar-Bourtchai L, Mariani-Kurkdjian P, Bingen E, Filliol I, Dhalluin A, Ifrane SA, Macrolide-resistant Shigella sonnei. Emerg Infect Dis. 2008;14:1297–9.
- Hassing RJ, Melles DC, Goessens WHF, Rijnders BJA. Case of Shigella flexneri infection with treatment failure due to azithromycin resistance in an HIV-positive patient. Infection. 2014;42:789–90.
- Jue S, Hardee R, Mays E, Bowen A, Whichard J, Greene K, ; Centers for Disease Control and Prevention (CDC). Notes from the field: emergence of Shigella flexneri 2a resistant to ceftriaxone and ciprofloxacin --- South Carolina, October 2010. MMWR Morb Mortal Wkly Rep. 2010;59:1619.