Volume 22, Number 12—December 2016
Letter
Zika Virus Infection in the Central Nervous System and Female Genital Tract
Emanuele Nicastri, Concetta Castilletti
, Pietro Balestra, Simonetta Galgani, and Giuseppe Ippolito
, Pietro Balestra, Simonetta Galgani, and Giuseppe Ippolito
Table
Virologic test results during Zika virus infection in a 32-year-old woman after she returned from the Dominican Republic to Italy, April–June 2016*
| Test/specimen type |
1st sample, day 6† |
2nd sample, day 7† |
3rd sample, day 10† |
4th sample, day 13† |
5th sample, day 17† |
6th sample, day 28† |
|---|---|---|---|---|---|---|
| Zika virus | ||||||
| rRT-PCR‡ serum | Positive (32.9) | Negative | Negative | Negative | Negative | Negative |
| rRT-PCR‡ urine | Positive (34.2) | Positive (31.8) | Positive (32.4) | Positive (29.8) | Positive (32.1) | Positive (32.2) |
| rRT-PCR‡ saliva | ND | Positive (29.9) | Positive (33.5) | Positive (34.1) | Negative | Negative |
| rRT-PCR‡ CSF | ND | Positive (37.0) | ND | ND | Negative | ND |
| rRT-PCR‡ cervical swab sample | ND | Positive (31.1) | Negative | Positive (34.3) | Negative | Negative |
| IFA§ IgM titer | <1:20 | 1:40 | 1:160 | 1:80 | 1:320 | 1:1,280 |
| IFA§ IgG titer | <1:20 | <1:20 | 1:40 | 1:320 | 1:320 | 1:320 |
| MNT¶ Ab titer |
ND |
ND |
1:40 |
ND |
1:160 |
>1:640 |
| Dengue virus IFA§ IgM titer | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 |
| Dengue virus IFA§ IgG titer |
<1:20 |
<1:20 |
<1:20 |
<1:20 |
<1:20 |
<1:20 |
| Chikungunya virus IFA§ IgM titer | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 |
| Chikungunya virus IFA§ IgG titer | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 | <1:20 |
*Ab, antibody; CSF, cerebrospinal fluid; IFA, immunofluorescence assay; MNT, microneutralization test; ND, not done; rRT-PCR, real-time reverse transcription PCR.
†Days from symptom onset.
‡Zika virus–specific rRT-PCR (RealStar Zika Virus RT-PCR Kit 1.0; Altona Diagnostics GmbH; Hamburg, Germany). Numbers in parentheses indicate cycle threshold values (Technical Appendix).
§IgG and IgM IFA (Arbovirus Mosaic 2; Euroimmun AG; Luebeck, Germany). Reference values (titer) serum: <1:20 = negative; >1:20 = positive (Technical Appendix).
¶MNT titers <1:20 were considered negative (Technical Appendix).
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Page updated: November 18, 2016
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