Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 22, Number 4—April 2016
Dispatch

Deletion Variants of Middle East Respiratory Syndrome Coronavirus from Humans, Jordan, 2015

Mart M. Lamers1, V. Stalin Raj1, Mah’d Shafei1, Sami Sheikh Ali, Sultan M. Abdallh, Mahmoud Gazo, Samer Nofal, Xiaoyan Lu, Dean D. Erdman, Marion Koopmans, Mohammad Abdallat, Bart L. HaagmansComments to Author , and Bart L. HaagmansComments to Author 
Author affiliations: Erasmus University Medical Center, Rotterdam, the Netherlands (M.M. Lamers, V.S. Raj, M.P. Koopmans, B.L. Haagmans); Jordan Ministry of Health, Amman, Jordan (M. Shafei, S.S. Ali, S.M. Abdallh, M. Gazo, S. Nofal, M. Abdallat, A. Haddadin); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, D.D. Erdman)

Main Article

Figure 2

Genomic characterization of Middle East respiratory syndrome coronaviruses (MERS-CoVs) from Jordan. A) Nucleotide differences between MERS-CoV strain Jordan-1-2015 and the consensus sequence of the Riyadh 2015 cluster. Nucleotide changes are indicated in green (A), red (T), blue (C), and black (G). Deletions are indicated in orange. Deleted nucleotides are indicated in red. B) Protein sequence alignment of open reading frame (ORF) 4a (residues 10–76) of all 13 MERS-CoV strains from Jordan in 201

Figure 2. Genomic characterization of Middle East respiratory syndrome coronaviruses (MERS-CoVs) from Jordan. A) Nucleotide differences between MERS-CoV strain Jordan-1-2015 and the consensus sequence of the Riyadh 2015 cluster. Nucleotide changes are indicated in green (A), red (T), blue (C), and black (G). Deletions are indicated in orange. Deleted nucleotides are indicated in red. B) Protein sequence alignment of open reading frame (ORF) 4a (residues 10–76) of all 13 MERS-CoV strains from Jordan in 2015 compared with the consensus sequence of the Riyadh 2015 cluster. No nucleotide substitutions were observed in this region between MERS-CoVs from Jordan. Predicted secondary structures are indicated (13). Classical double-stranded RNA binding proteins have αβββα architecture. C) Protein sequence alignment of ORF3 (residues 25–77) of all 13 MERS-CoV strains from Jordan in 2015 compared with the consensus sequence of the Riyadh 2015 cluster. No nucleotide substitutions were observed in this region between MERS-CoVs from Jordan. Alignments were generated with the ClustalW program (http://pbil.univ-lyon1.fr/software/seaview) and visualized by using Jalview 2.9 (http://www.jalview.org/). Boxes in panels B and C indicate regions where amino acids have been deleted in the viruses from Jordan.

Main Article

References
  1. Zaki  AM, van Boheemen  S, Bestebroer  TM, Osterhaus  AD, Fouchier  RA. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med. 2012;367:1814–20. DOIPubMedGoogle Scholar
  2. World Health Organization. Emergencies preparedness, response: Middle East respiratory syndrome coronavirus (MERS-CoV) maps and epicurves [cited 2015 Nov 18]. http://www.who.int/csr/disease/coronavirus_infections/maps-epicurves/
  3. Drosten  C, Meyer  B, Müller  MA, Corman  VM, Al-Masri  M, Hossain  R, Transmission of MERS-coronavirus in household contacts. N Engl J Med. 2014;371:828–35. DOIPubMedGoogle Scholar
  4. Assiri  A, McGeer  A, Perl  TM, Price  CS, Al Rabeeah  AA, Cummings  DA, Hospital outbreak of Middle East respiratory syndrome coronavirus. N Engl J Med. 2013;369:407–16. DOIPubMedGoogle Scholar
  5. Cotten  M, Watson  SJ, Kellam  P, Al-Rabeeah  AA, Makhdoom  HQ, Assiri  A, Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet. 2013;382:1993–2002. DOIPubMedGoogle Scholar
  6. Drosten  C, Muth  D, Corman  VM, Hussain  R, Al Masri  M. HajOmar W, et al. An observational, laboratory-based study of outbreaks of Middle East respiratory syndrome coronavirus in Jeddah and Riyadh, Kingdom of Saudi Arabia, 2014. Clin Infect Dis. 2015;60:369–77.
  7. Fagbo  SF, Skakni  L, Chu  DKW, Garbati  MA, Joseph  M, Peiris  M, Molecular epidemiology of hospital outbreak of Middle East respiratory syndrome, Riyadh, Saudi Arabia, 2014. Emerg Infect Dis [cited 2015 Oct 27]. DOIGoogle Scholar
  8. Seong  MW, Kim  SY, Corman  VM, Kim  TS, Cho  SI, Kim  MJ, Microevolution of outbreak-associated Middle East respiratory syndrome coronavirus, South Korea, 2015. Emerg Infect Dis [cited 2015 Nov 29]. DOIGoogle Scholar
  9. Chinese SARS Molecular Consortium. Molecular evolution of the SARS coronavirus during the course of the SARS epidemic in China. Science. 2004;303:1666–9. DOIPubMedGoogle Scholar
  10. World Health Organization. Global alert and response (GAR). Middle East respiratory syndrome coronavirus (MERS-CoV)—Jordan [cited 2015 Nov 18]. http://www.who.int/csr/don/01-september-2015-mers-jordan/en/
  11. Haagmans  BL, Al Dhahiry  SH, Reusken  CB, Raj  VS, Galiano  M, Myers  R, Middle East respiratory syndrome coronavirus in dromedary camels: an outbreak investigation. Lancet Infect Dis. 2014;14:140–5 and. DOIPubMedGoogle Scholar
  12. van Boheemen  S, de Graaf  M, Lauber  C, Bestebroer  TM, Raj  VS, Zaki  AM, Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans. MBio. 2012;3:e00473–12. DOIPubMedGoogle Scholar
  13. Siu  KL, Yeung  ML, Kok  KH, Yuen  KS, Kew  C, Lui  PY, Middle East respiratory syndrome coronavirus 4a protein is a double-stranded RNA-binding protein that suppresses PACT-induced activation of RIG-I and MDA5 in the innate antiviral response. J Virol. 2014;88:4866–76. DOIPubMedGoogle Scholar
  14. Niemeyer  D, Zillinger  T, Muth  D, Zielecki  F, Horvath  G, Suliman  T, Middle East respiratory syndrome coronavirus accessory protein 4a is a type I interferon antagonist. J Virol. 2013;87:12489–95. DOIPubMedGoogle Scholar

Main Article

1These authors contributed equally to this article.

Page created: March 16, 2016
Page updated: March 16, 2016
Page reviewed: March 16, 2016
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external