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Volume 22, Number 4—April 2016
Dispatch

Deletion Variants of Middle East Respiratory Syndrome Coronavirus from Humans, Jordan, 2015

Mart M. Lamers1, V. Stalin Raj1, Mah’d Shafei1, Sami Sheikh Ali, Sultan M. Abdallh, Mahmoud Gazo, Samer Nofal, Xiaoyan Lu, Dean D. Erdman, Marion Koopmans, Mohammad Abdallat, Bart L. HaagmansComments to Author , and Bart L. HaagmansComments to Author 
Author affiliations: Erasmus University Medical Center, Rotterdam, the Netherlands (M.M. Lamers, V.S. Raj, M.P. Koopmans, B.L. Haagmans); Jordan Ministry of Health, Amman, Jordan (M. Shafei, S.S. Ali, S.M. Abdallh, M. Gazo, S. Nofal, M. Abdallat, A. Haddadin); Centers for Disease Control and Prevention, Atlanta, Georgia, USA (X. Lu, D.D. Erdman)

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Figure 2

Genomic characterization of Middle East respiratory syndrome coronaviruses (MERS-CoVs) from Jordan. A) Nucleotide differences between MERS-CoV strain Jordan-1-2015 and the consensus sequence of the Riyadh 2015 cluster. Nucleotide changes are indicated in green (A), red (T), blue (C), and black (G). Deletions are indicated in orange. Deleted nucleotides are indicated in red. B) Protein sequence alignment of open reading frame (ORF) 4a (residues 10–76) of all 13 MERS-CoV strains from Jordan in 201

Figure 2. Genomic characterization of Middle East respiratory syndrome coronaviruses (MERS-CoVs) from Jordan. A) Nucleotide differences between MERS-CoV strain Jordan-1-2015 and the consensus sequence of the Riyadh 2015 cluster. Nucleotide changes are indicated in green (A), red (T), blue (C), and black (G). Deletions are indicated in orange. Deleted nucleotides are indicated in red. B) Protein sequence alignment of open reading frame (ORF) 4a (residues 10–76) of all 13 MERS-CoV strains from Jordan in 2015 compared with the consensus sequence of the Riyadh 2015 cluster. No nucleotide substitutions were observed in this region between MERS-CoVs from Jordan. Predicted secondary structures are indicated (13). Classical double-stranded RNA binding proteins have αβββα architecture. C) Protein sequence alignment of ORF3 (residues 25–77) of all 13 MERS-CoV strains from Jordan in 2015 compared with the consensus sequence of the Riyadh 2015 cluster. No nucleotide substitutions were observed in this region between MERS-CoVs from Jordan. Alignments were generated with the ClustalW program (http://pbil.univ-lyon1.fr/software/seaview) and visualized by using Jalview 2.9 (http://www.jalview.org/). Boxes in panels B and C indicate regions where amino acids have been deleted in the viruses from Jordan.

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1These authors contributed equally to this article.

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