Isolation of Zika Virus from Febrile Patient, Indonesia
Aditya Perkasa, Frilasita Yudhaputri, Sotianingsih Haryanto, Rahma F. Hayati, Chairin Nisa Ma’roef, Ungke Antonjaya, Benediktus Yohan, Khin Saw Aye Myint, Jeremy P. Ledermann, Ronald Rosenberg, Ann M. Powers, and R. Tedjo Sasmono
Author affiliations: Eijkman Institute for Molecular Biology, Jakarta, Indonesia (A. Perkasa, F. Yudhaputri, R.F. Hayati, C.N. Ma’roef, B. Yohan, K.S.A. Myint, R.T. Sasmono); Siloam Hospitals Jambi, Jambi, Indonesia (S. Haryanto); Eijkman-Oxford Clinical Research Unit, Jakarta (U. Antonjaya); Centers for Disease Control and Prevention, Fort Collins, Colorado, USA (J.P. Ledermann, R. Rosenberg, A.M. Powers)
Figure. Phylogenetic tree comparing Zika virus isolate from a patient in Indonesia (ID/JMB-185/2014; arrow) to reference strains from GenBank (accession numbers indicated). The tree was constructed from nucleic acid sequences of 530 bp from the nonstructural protein 5 region by using the minimum evolution algorithm in MEGA 6 (http://www.megasoftware.net). Numbers to the left of the nodes are bootstrap percentages (2,000 replications). Bootstrap values <70 are not shown. The tree was rooted with the Spondweni virus isolated in South Africa as the outgroup. Scale bar indicates nucleotide substitutions per site.
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