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Volume 22, Number 6—June 2016
Dispatch

Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus

Gary Crameri, Peter A. DurrComments to Author , Reuben Klein, Adam Foord, Meng Yu, Sarah Riddell, Jessica Haining, Dayna Johnson, Maged G. Hemida, Jennifer Barr, Myoung-don Oh, Deborah Middleton, and Lin-Fa Wang
Author affiliations: CSIRO Australian Animal Health Laboratory, Geelong, Victoria, Australia (G. Crameri, P.A. Durr, R. Klein, A. Foord, M. Yu, S. Riddell, J. Haining, D. Johnson, J. Barr, D. Middleton); Kafrelsheikh University, Kafr Elsheikh, Egypt (M.G. Hemida); King Faisal University, Hofuf, Saudi Arabia (M.G. Hemida); University of Hong Kong, Hong Kong, China (M. Peiris); Duke–National University of Singapore Medical School, Singapore (L.-F. Wang)

Main Article

Table 2

Serologic responses in 3 infected alpacas, as measured by virus neutralization tests and Luminex bead assays for selected sample days*

Dpi Serologic results
 No. positive/no. tested)§
VNT titer†
 Luminex assay MFI‡
Alpaca 1 Alpaca 2 Alpaca 3 Alpaca 1 Alpaca 2 Alpaca 3 VNT Luminex
0 Negative Negative Negative 787 889 167 0/3 0/3
3 Negative Negative Negative 373 814 152 0/3 0/3
5 Negative Negative Negative 418 945 223 0/3 0/3
7 Negative Negative Negative 272 932 249 0/3 0/3
10 Negative 1:40 Negative 478 2,869 58 1/3 1/3
12 Negative 1:40 Negative 928 10,274 331 1/3 1/3
14 Negative 1:40 Negative 1,041 7,658 899 1/3 1/3
21 1:10 1:40 Negative 877 6,893 629 1/3 1/3
24 1:20 1:20 Negative 1,506 3,324 678 2/3 1/3
26 1:20 1:20 Negative 853 4,161 667 2/3 1/3
28 1:20 1:20 Negative 773 4,682 724 2/3 1/3
31 1:20 1:40 Negative 548 11,259 649 2/3 1/3
33 1:10 1:20 Negative 688 6,090 455 1/3 1/3
35§ 1:10 NA Negative 510 NA 586 0/2 0/2

*Bold indicates positive results. Gray shading indicates that >1 animal was positive for the sample collected on that day. Dpi, day postinfection for challenge (initial infection); MFI, median fluorescent intensity; VNT, virus neutralization test.
†The starting dilution was 1:10, and the threshold for a positive result was a dilution of >1:20.
‡The MFI threshold for a positive result for the Luminex assay was 2,500.
§Alpaca 2 was euthanized at day 33, leaving only 2 animals in the study at day 35.

Main Article

Page created: May 16, 2016
Page updated: May 16, 2016
Page reviewed: May 16, 2016
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