Volume 22, Number 9—September 2016
Dispatch
Borrelia miyamotoi–Associated Neuroborreliosis in Immunocompromised Person
Figure 1

Figure 1. Molecular phylogenetic analysis of Borrelia strain from cerebrospinal fluid of a 74-year-old immunocompromised woman in Germany (black dot) conducted by using 6 multilocus sequence typing genes (clpA, clpX, pepX, pyrG, recG, rplB) of Borrelia miyamotoi. The sequences obtained from the patient sample clustered with B. miyamotoi strain EU_T01 from Europe, retrieved from the PubMLST Borrelia database (http://pubmlst.org/borrelia/). The phylogenetic relationship of the sample analyzed was inferred by using DNA sequences of chromosomal housekeeping genes. The maximum-likelihood method based on the general time reversible model (8) was applied. The tree with the highest log likelihood (–5531.9051) is shown. The percentage of trees in which the associated taxa clustered is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying neighbor-joining and BioNJ algorithms (http://bionj.org/about-bionj) to a matrix of pairwise distances estimated by using the maximum composite likelihood approach and then selecting the topology with superior log likelihood value. The tree is drawn to scale; branch lengths are measured in the number of substitutions per site. The analysis involved 5-nt sequences. Codon positions included were 1st+2nd+3rd+Noncoding. All positions containing gaps and missing data were eliminated. The final dataset contained 3,642 positions. Evolutionary analyses were conducted in MEGA5 (9).
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