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Volume 23, Number 1—January 2017

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Dolphin Morbillivirus Associated with a Mass Stranding of Sperm Whales, Italy

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EID Mazzariol S, Centelleghe C, Di Provvido A, Di Renzo L, Cardeti G, Cersini A, et al. Dolphin Morbillivirus Associated with a Mass Stranding of Sperm Whales, Italy. Emerg Infect Dis. 2017;23(1):144-146. https://dx.doi.org/10.3201/eid2301.160239
AMA Mazzariol S, Centelleghe C, Di Provvido A, et al. Dolphin Morbillivirus Associated with a Mass Stranding of Sperm Whales, Italy. Emerging Infectious Diseases. 2017;23(1):144-146. doi:10.3201/eid2301.160239.
APA Mazzariol, S., Centelleghe, C., Di Provvido, A., Di Renzo, L., Cardeti, G., Cersini, A....Di Guardo, G. (2017). Dolphin Morbillivirus Associated with a Mass Stranding of Sperm Whales, Italy. Emerging Infectious Diseases, 23(1), 144-146. https://dx.doi.org/10.3201/eid2301.160239.

Abstract

In September 2014, seven sperm whales were stranded along Italy’s Adriatic coastline. Postmortem investigations on 3 female adult whales and 1 male fetus carried by the largest female revealed molecular and immunohistochemical evidence of dolphin morbillivirus infection. A possible role of the virus in the stranding event was considered.

The mass strandings of sperm whales (Physeter macrocephalus) are still largely unexplained events. Solar cycles, weather conditions, coastal geographic features, and human activities have been proposed as possible causes (1). Although a multifactorial etiology was hypothesized to be responsible for a mass stranding that occurred in December 2009 involving 7 male sperm whales along the southern Adriatic coast of Italy (1), well-defined causes of similar events are rarely identified.

In September 2014, seven sperm whales were found stranded along the central Adriatic Italian coastline; 4 of these whales were refloated. Three females (sperm whale 1 [SW1], pregnant, total length 8.95 m; SW2, total length 8.38 m; and SW3, total length 7.33 m) died on the beach, and postmortem analyses revealed that the largest animal, a pregnant female (SW1), whose male fetus (SW1b) also died, was affected by a prominent hydronephrosis caused by a large stone occupying >50% of the right kidney pelvis. This condition might have resulted in renal function impairment. We observed microscopically evidence of lymphoid cell depletion in several secondary lymphoid tissues from all 3 adult whales, and we found definitive biomolecular evidence of dolphin morbillivirus (DMV) infection in the 3 adult whales and in the fetus.

Figure

Thumbnail of Spleen of the youngest female sperm whale (SW3) in study of 7 sperm whales stranded along Italy’s Adriatic coastline in September 2014. Positive immunostaining (Mayer’s hematoxylin counterstain) for morbilliviral antigen is shown in monocytes within vascular lumina and in follicular dendritic-like cells in the splenic white pulp. Morbillivirus immunohistochemistry was conducted with a murine monoclonal antibody against canine distemper virus nucleoprotein (VMRD Inc., Pullman, WA, US

Figure. Spleen of the youngest female sperm whale (SW3) in study of 7 sperm whales stranded along Italy’s Adriatic coastline in September 2014. Positive immunostaining (Mayer’s hematoxylin counterstain) for morbilliviral antigen is...

Our use of 1-step PCR protocols (2) failed to sequence any viral fragment, probably because of postmortem changes and subsequent viral RNA degradation by ribonucleases, which progress rapidly in large whales because of their body features and size and thus affect the integrity of the DMV genome. Therefore, we used a more sensitive, nested reverse transcription PCR technique that targeted a highly preserved fragment of the viral hemagglutinin (H) gene (3) to investigate the lung (SW1), brain (SW3), and lymphoid tissues (SW2 and SW3) of the 3 adult whales and the lung, kidney, and liver of the fetus (SW1b). We found all of these tissues to be positive for DMV, and we analyzed the gene sequences obtained from the 4 whales’ tissues by using an ad hoc computer program (Lasergene package version SeqMan Pro; DNASTAR Inc., Madison, WI, USA). The hemagglutinin consensus fragment obtained from all the positive samples showed 100% sequence homology with the corresponding DMV genome sequence (GenBank accession no. AJ608288). In addition, we observed simultaneous immunohistochemical (IHC) evidence of DMV nucleoprotein antigen in macrophages and follicular dendritic-like cells from the white pulp of the spleen from the youngest female whale (SW3) and in monocytes circulating within splenic blood vessels from the same animal (Figure). Other target tissues from this whale and the 3 others were either negative or unsuitable for IHC and biomolecular analyses.

Although we did not observe any classic DMV-related pathologic changes during postmortem examinations (2,4), we strongly suspected a viremic condition in all 4 whales on the basis of IHC (SW3) and biomolecular (SW1, SW1b, SW2, and SW3) findings. In this respect, the consistent immunolabeling of morbillivirus antigens in circulating monocytes and in splenic follicular-like dendritic cells support the hypothesis that DMV infection was in an early developmental stage (5). Experimental studies conducted on similar morbillivirus infection models show that during this period, even if no severe clinical signs are observed, a general discomfort condition, secondary to viremic circulation, could be reasonably expected (6,7).

DMV infection has been frequently associated with mass deaths during epidemic outbreaks (2); however, it has been seldom reported in single mass stranding events among cetaceans in general, much less among sperm whales. Furthermore, given the documented susceptibility of sperm whales to cetacean morbillivirus and the likelihood of maternal–fetal transfer of the virus (8), the biomolecular evidence of DMV infection obtained in the fetal sperm whale in our investigation strongly supports the assumption of a transplacentally acquired infection in this animal.

Although no clear-cut evidence exists that DMV was the primary cause of the mass stranding of sperm whales we report, a vast body of scientific literature is available to support the primary pathogenicity of morbillivirus genus members for their mammalian hosts, including aquatic mammals (3). In this respect, previous studies would suggest that chemical pollutants, especially methylmercury, were a relevant causative factor in the stranding and subsequent deaths of the 3 sperm whales in our study (9). However, our findings suggest the possible additional role of the DMV infection in this event because it is one of the factors that could explain the whales’ northward trajectory into the Adriatic Sea, which is known to be a cul-de-sac for this species (1).

In conclusion, although a vast body of scientific data supports the direct involvement of cetacean morbillivirus clade members such as DMV in the numerous epidemics that have occurred in the last 25–30 years among free-ranging cetaceans worldwide (2), their causative role in single mass stranding events cannot be precisely defined. Several additional biologic, ecologic, and environmental factors should be investigated by using a multidisciplinary approach (10).

Dr. Mazzariol is an assistant professor in the Department of Comparative Biomedicine and Food Science of the University of Padova. His primary research interests are diseases and pathologies of marine mammals.

Acknowledgments

We thank Bruno Cozzi for providing archival tissues from the Mediterranean Marine Mammal Tissue Bank; Michele Povinelli and Emanuele Zanetti for their invaluable technical work during necropsies in the field; and Vincenzo Olivieri and staff from Centro Studi Cetacei for their logistic support and their efforts during the sperm whales’ refloatation.

This study was funded by Italy’s Ministry for the Environment, Land, and Sea.

Sandro MazzariolComments to Author , Cinzia Centelleghe, Andrea Di Provvido, Ludovica Di Renzo, Giusy Cardeti, Antonella Cersini, Gianluca Fichi, Antonio Petrella, Cristina Esmeralda Di Francesco, Walter Mignone, Cristina Casalone, and Giovanni Di Guardo

Author affiliations: University of Padova Department of Comparative Biomedicine and Food Science, Padua, Italy (S. Mazzariol, C. Centelleghe); Istituto Zooprofilattico Sperimentale dell’Abruzzo e Molise, Teramo, Italy (A. Di Provvido, L. Di Renzo); Istituto Zooprofilattico Sperimentale del Lazio e della Toscana, Rome, Italy (G. Cardeti, A. Cersini); Istituto Zooprofilattico Sperimentale del Lazio e della Toscana, Pisa, Italy (G. Fichi); Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Foggia, Italy (A. Petrella); University of Teramo Faculty of Veterinary Medicine, Teramo (G. Di Guardo G., C.E. Di Francesco); Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Imperia, Italy (W. Mignone); Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d’Aosta, Turin, Italy (C. Casalone)

References

  1. Mazzariol S, Di Guardo G, Petrella A, Marsili L, Fossi CM, Leonzio C, et al. Sometimes sperm whales (Physeter macrocephalus) cannot find their way back to the high seas: a multidisciplinary study on a mass stranding. PLoS One. 2011;6:e19417. DOIPubMed
  2. Centelleghe C, Beffagna G, Zanetti R, Zappulli V, Di Guardo G, Mazzariol S. Molecular analysis of dolphin morbillivirus: A new sensitive detection method based on nested RT-PCR. J Virol Methods. 2016;235:8591. DOIPubMed
  3. Van Bressem MF, Duignan PJ, Banyard A, Barbieri M, Colegrove KM, De Guise S, et al. Cetacean morbillivirus: current knowledge and future directions. Viruses. 2014;6:514581. DOIPubMed
  4. Stephens N, Duignan PJ, Wang J, Bingham J, Finn H, Bejder L, et al. Cetacean morbillivirus in coastal Indo-Pacific bottlenose dolphins, Western Australia. Emerg Infect Dis. 2014;20:66670. DOIPubMed
  5. de Swart RL, Ludlow M, de Witte L, Yanagi Y, van Amerongen G, McQuaid S, et al. Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. PLoS Pathog. 2007;3:e178. DOIPubMed
  6. de Vries RD, Mesman AW, Geijtenbeek TB, Duprex WP, de Swart RL. The pathogenesis of measles. Curr Opin Virol. 2012;2:24855. DOIPubMed
  7. Stein VM, Schreiner NM, Moore PF, Vandevelde M, Zurbriggen A, Tipold A. Immunophenotypical characterization of monocytes in canine distemper virus infection. Vet Microbiol. 2008;131:23746. DOIPubMed
  8. West KL, Levine G, Jacob J, Jensen B, Sanchez S, Colegrove K, et al. Coinfection and vertical transmission of Brucella and Morbillivirus in a neonatal sperm whale (Physeter macrocephalus) in Hawaii, USA. J Wildl Dis. 2015;51:22732. DOIPubMed
  9. Squadrone S, Chiaravalle E, Gavinelli S, Monaco G, Rizzi M, Abete MC. Analysis of mercury and methylmercury concentrations, and selenium:mercury molar ratios for a toxicological assessment of sperm whales (Physeter macrocephalus) in the most recent stranding event along the Adriatic coast (Southern Italy, Mediterranean Sea). Chemosphere. 2015;138:63341. DOIPubMed
  10. Mazzariol S, Marsili L, Di Guardo G. Cetacean mass strandings and multidisciplinary work. Chemosphere. 2016;148:323. DOIPubMed

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DOI: 10.3201/eid2301.160239

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Table of Contents – Volume 23, Number 1—January 2017

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Sandro Mazzariol, Department of Comparative Biomedicine and Food Science, University of Padua, Viale dell’Università 16, 35020 Legnaro (PD), Italy


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