Skip directly to site content Skip directly to page options Skip directly to A-Z link Skip directly to A-Z link Skip directly to A-Z link
Volume 23, Number 11—November 2017
Research Letter

Blood Culture–Negative Endocarditis, Morocco

Tables
Article Metrics
3
citations of this article
EID Journal Metrics on Scopus
Najma BoudebouchComments to Author , M’hammed Sarih, Abdelfattah Chakib, Salma Fadili, Drissi Boumzebra, Zahira Zouizra, Badie Azamane Mahadji, Hamid Amarouch, Didier Raoult, and Pierre-Edouard Fournier
Author affiliations: Institut Pasteur du Maroc, Casablanca, Morocco (N. Boudebouch, M. Sarih); Centre Hospitalier Universitaire Ibn Rochd, Casablanca (A. Chakib, S. Fadili, B.A. Mahadji); Centre Hospitalier Universitaire Ibn Toufail Marrakech, Marrakech, Morocco (D. Boumzebra, Z. Zouizra); Faculté des Sciences Ain Chock, Casablanca (H. Amarouch); Aix-Marseille Université, Assistance Publique-Hôpitaux de Marseille, Marseille, France (D. Raoult, P.-E. Fournier)

Cite This Article

Abstract

We investigated the microorganisms causing blood culture–negative endocarditis (BCNE) in Morocco. We tested 19 patients with BCNE by serologic methods, molecular methods, or both and identified Bartonella quintana, Staphylococcus aureus, Streptococcus equi, and Streptococcus oralis in 4 patients. These results highlight the role of these zoonotic agents in BCNE in Morocco.

Blood culture–negative endocarditis (BCNE) can occur when the patient has previously received antibiotic drugs or in the presence of slow-growing or intracellular microorganisms (1,2). In Morocco, epidemiologic data on endocarditis are fragmentary and show that this disease is frequently associated with rheumatic heart disease (3). The aim of this multicenter preliminary study was to investigate the microorganisms causing BCNE in Morocco by using a multimodal strategy.

During June 1, 2009–September 1, 2010, we prospectively included all patients with BCNE seen in 3 health centers in the Morocco regions of Casablanca and Marrakech. The specimens were then referred to the Institut Pasteur du Maroc in Casablanca before being transported to Marseille, France, where diagnostic assays were performed as described by Fournier et al. (4). For each studied patient, the physician in charge completed a questionnaire. Answers were not obtained for all questions from all patients.

We used indirect immunofluorescence assays to detect significant levels of antibodies to Coxiella burnetii IgG titer to phase I ≥1:800), Bartonella quintana, B. henselae (IgG titer ≥1:800), and Legionella pneumophila (total antibody titer >1:256), as previously described (4). We detected specific antibodies to Brucella melitensis by using an immunoenzymatic antibody test (titer >1:200) and to Mycoplasma pneumoniae by using the Platelia M. pneumonia IgM kit (Bio-Rad, Marnes-la-Coquette, France). When results of first-rank tests were negative, we systematically performed the Western blot test by using Bartonella antigens (4).

We extracted bacterial DNA from excised valves or EDTA blood by using the QIAmp Tissue kit (QIAGEN, Hilden, Germany) and performed PCR (5). We examined paraffin-embedded heart valves and used hematoxylin and eosin stain for histopathologic features (5). To detect microorganisms within tissues, we systematically performed the Giemsa, Gram (Brown-Brenn and Brown Hopps), periodic-acid Schiff, Grocott-Gomori, Warthin-Starry, Gimenez, and Ziehl-Nielsen stains (4). For patients for whom results of all other techniques remained negative, we performed autoimmunohistochemistry as described by Lepidi et al. (6).

Our prospective study enabled the identification of 19 patients (Table): 11 men, 7 women, and 1 person of unspecified gender. Mean age was 40.26 years (range 22–57 years). Among these, 6 lived in urban areas (socioeconomic conditions unknown) and 7 in periurban communities under conditions of poverty. No information on residential environment could be obtained for the remaining 6 patients. All patients except 1 had received antibacterial drugs before blood sampling.

Samples from all 19 BCNE patients were tested by serologic methods, molecular methods, or both. Among these, we identified an etiologic agent for 4 patients. A 48-year-old man living in impoverished conditions had a positive Bartonella serologic test result (IgG 1:6400); Western blot analysis of the serum sample resulted in the specific diagnosis of B. quintana infection. In addition, 3 cardiac valves from 3 patients tested by using 16S rRNA PCR were positive, 1 each for Staphylococcus aureus, Streptococcus equi, and Streptococcus oralis. PCR performed with a second gene confirmed all 3 PCR results. None of these 3 patients lived in impoverished areas.

In Morocco, cases of BCNE represent two thirds of all cases of infectious endocarditis and constitute a major problem of diagnosis and management of patients (1). In other countries, the negativity of blood cultures in BCNE may be explained mostly by the administration of antimicrobial drugs before blood culture collection or by the causative role of fastidious microorganisms, as is the case for zoonotic pathogens, such as C. burnetti, B. quintana, and B. henselae (7). However, in Morocco, cases of endocarditis caused by these zoonotic pathogens are poorly diagnosed. Previous studies have demonstrated the role of C. burnetii, but we could find no study that directly detected Bartonella in BCNE.

In the neighboring country of Algeria, B. quintana is considered to be the most common agent of infectious endocarditis, with a prevalence of 15.6%, compared with 5% in countries in Europe (8). This difference is likely explained by differences in living conditions (9). B. quintana infections occur preferentially in disadvantaged populations (homeless) infected by body lice. In our study, 7 patients for whom information on their residential environment was available lived in conditions of poverty and poor hygiene, including the patient who had B. quintana endocarditis.

Bartonella spp. can infect any heart valve and cause destructive valvular lesions often requiring surgical replacement. Despite the high antimicrobial drug susceptibility of these bacteria, the mortality rate from Bartonella endocarditis may reach 31% (10). In addition, the recommended treatment for these infections is a combination of doxycycline and gentamicin, which is different from that for endocarditis caused by other common bacterial species, and therefore a specific diagnosis is highly desirable (10). However, the small number of patients included in our study precludes the issuance of a definitive recommendation to include serologic testing, molecular testing, or both for zoonotic pathogens in patients with endocarditis in Morocco.

Dr. Boudebouch is a researcher at the Institut Pasteur du Maroc, Morocco. Her primary research interest is in emerging and zoonotic diseases including diagnostics and molecular microbiology of some fastidious bacteria.

Top

Acknowledgments

We thank all clinicians who voluntarily participated in this study.

This work was funded by the Mediterranee-Infection Foundation and the French Agence Nationale de la Recherche under reference Méditerranée Infection 10-IAHU-03.

Top

References

  1. Brouqui  P, Raoult  D. Endocarditis due to rare and fastidious bacteria. Clin Microbiol Rev. 2001;14:177207. DOIPubMed
  2. Sekkali  N, Agharbi  S, Ait El Kadi  M, Lahlou  I, Ouaha  L, Akoudad  H. Infective endocarditis (part 1). Pathophysiology, diagnosis [in French]. Le Journal Marocain de Cardiologie IV [cited 6/27/2017]. http://www.moroccanjournalofcardiology.org/Edition-4/pdf/FMC.pdf
  3. Raoult  D, Casalta  JP, Richet  H, Khan  M, Bernit  E, Rovery  C, et al. Contribution of systematic serological testing in diagnosis of infective endocarditis. J Clin Microbiol. 2005;43:523842. DOIPubMed
  4. Fournier  PE, Thuny  F, Richet  H, Lepidi  H, Casalta  JP, Arzouni  JP, et al. Comprehensive diagnostic strategy for blood culture-negative endocarditis: a prospective study of 819 new cases. Clin Infect Dis. 2010;51:13140. DOIPubMed
  5. Houpikian  P, Raoult  D. Blood culture-negative endocarditis in a reference center: etiologic diagnosis of 348 cases. Medicine (Baltimore). 2005;84:16273. DOIPubMed
  6. Lepidi  H, Durack  DT, Raoult  D. Diagnostic methods current best practices and guidelines for histologic evaluation in infective endocarditis. [ix.]. Infect Dis Clin North Am. 2002;16:33961, ix. DOIPubMed
  7. Sanae  A. L’endocardite infectieuse: analyse retrospective de 100 cas colliges au service de cardiologie du chu II de Fès (thèse) [in French]. [cited 6/27/2017]. http://docplayer.fr/8362537-L-endocardite-infectieuse-analyse-retrospective-de-100-cas-colliges-au-service-de-cardiologie-du-chu-ii-de-fes.htm
  8. Benslimani  A, Fenollar  F, Lepidi  H, Raoult  D. Bacterial zoonoses and infective endocarditis, Algeria. Emerg Infect Dis. 2005;11:21624. DOIPubMed
  9. Raoult  D, Fournier  PE, Drancourt  M, Marrie  TJ, Etienne  J, Cosserat  J, et al. Diagnosis of 22 new cases of Bartonella endocarditis. Ann Intern Med. 1996;125:64652. DOIPubMed
  10. Raoult  D, Fournier  PE, Vandenesch  F, Mainardi  JL, Eykyn  SJ, Nash  J, et al. Outcome and treatment of Bartonella endocarditis. Arch Intern Med. 2003;163:22630. DOIPubMed

Top

Table

Top

Cite This Article

DOI: 10.3201/eid2311.161066

Table of Contents – Volume 23, Number 11—November 2017

Comments

Please use the form below to submit correspondence to the authors or contact them at the following address:

Najma Boudebouch, Département de Recherche, Institut Pasteur du Maroc 1, Place Louis Pasteur, 20360 Casablanca, Morocco; or

Send To

character(s) remaining.

Comment submitted successfully, thank you for your feedback.

Top

Page created: October 17, 2017
Page updated: October 17, 2017
Page reviewed: October 17, 2017
The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
file_external